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Aqueous humor enhances the proliferation of rat retinal precursor cells in culture, and this effect is partially reproduced by ascorbic acid
Yang, Jing; Klassen, Henry; Pries, Mette; Wang, Wei; Nissen, Mogens H.
关键词Retinal Precursor Cells Aqueous Humor Ascorbic Acid Proliferation Low Molecular Mass Protein
刊名STEM CELLS
2006-12-01
DOI10.1634/stemcells.2006-0103
24期:12页:2766-2775
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell & Tissue Engineering ; Biotechnology & Applied Microbiology ; Oncology ; Cell Biology ; Hematology
研究领域[WOS]Cell Biology ; Biotechnology & Applied Microbiology ; Oncology ; Hematology
关键词[WOS]FIBROBLAST GROWTH-FACTOR ; DYSTROPHIC RCS RAT ; ROD OUTER SEGMENTS ; NEURAL STEM-CELLS ; PROGENITOR CELLS ; PIGMENT-EPITHELIUM ; PHOTORECEPTOR DEGENERATION ; SUBRETINAL FLUID ; IN-VITRO ; DIFFERENTIATION
英文摘要

Aqueous humor has been shown to influence the proliferation of various ocular cell types, but the effect on immature retinal cells is not known. Here, the effect of pig aqueous humor on the proliferation of rat retinal precursor cells (RPCs) was investigated. RPCs were prepared from embryonic day 19 Sprague-Dawley rats and cultured in the presence or absence of aqueous humor from healthy pigs along with a medium consisting of Dulbecco′s modified Eagle′s medium: Ham′s F-12 medium, N2 supplement, and epidermal growth factor. Proliferation was quantified by [H-3] thymidine incorporation under different treatment conditions, and any associated morphological changes were noted. Potential active components of porcine aqueous humor were partially characterized by gel filtration chromatography, and the effect on RPC proliferation was determined. Results showed that adding 20% aqueous humor increased [H-3] thymidine incorporation by as much as 317%, as compared with controls. Aqueous supplementation also increased both the number and size of RPC spherical aggregates ("spheres") over the first 4 days, consistent with increased proliferative activity. Using gel filtration and the in vitro proliferation assay, the growth- promoting activity of aqueous humor was localized to two different molecular mass ranges, namely, around 30 kDa and less than 1 kDa. Ascorbic acid was present in the lower molecular mass fraction, and use of this molecule reproduced some, but not all, of the proliferative activity present in aqueous humor. These results highlight the potential role of soluble factors present in the cellular microenvironment with respect to modulation of endogenous progenitor cell activity.

语种英语
WOS记录号WOS:000242696200018
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被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/55967
专题北京大学第三临床医学院_眼科
作者单位1.Univ Copenhagen, Panum Inst, Dept Med Anat, Expt Immunol Lab, DK-2200 Copenhagen, Denmark
2.Peking Univ, Hosp 3, Dept Ophthalmol, Beijing 100871, Peoples R China
3.Singapore Eye Res Inst, Singapore, Singapore
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GB/T 7714
Yang, Jing,Klassen, Henry,Pries, Mette,et al. Aqueous humor enhances the proliferation of rat retinal precursor cells in culture, and this effect is partially reproduced by ascorbic acid[J]. STEM CELLS,2006,24(12):2766-2775.
APA Yang, Jing,Klassen, Henry,Pries, Mette,Wang, Wei,&Nissen, Mogens H..(2006).Aqueous humor enhances the proliferation of rat retinal precursor cells in culture, and this effect is partially reproduced by ascorbic acid.STEM CELLS,24(12),2766-2775.
MLA Yang, Jing,et al."Aqueous humor enhances the proliferation of rat retinal precursor cells in culture, and this effect is partially reproduced by ascorbic acid".STEM CELLS 24.12(2006):2766-2775.
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