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学科主题: 临床医学
题名:
Aqueous humor enhances the proliferation of rat retinal precursor cells in culture, and this effect is partially reproduced by ascorbic acid
作者: Yang, Jing; Klassen, Henry; Pries, Mette; Wang, Wei; Nissen, Mogens H.
关键词: retinal precursor cells ; aqueous humor ; ascorbic acid ; proliferation ; low molecular mass protein
刊名: STEM CELLS
发表日期: 2006-12-01
DOI: 10.1634/stemcells.2006-0103
卷: 24, 期:12, 页:2766-2775
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell & Tissue Engineering ; Biotechnology & Applied Microbiology ; Oncology ; Cell Biology ; Hematology
研究领域[WOS]: Cell Biology ; Biotechnology & Applied Microbiology ; Oncology ; Hematology
关键词[WOS]: FIBROBLAST GROWTH-FACTOR ; DYSTROPHIC RCS RAT ; ROD OUTER SEGMENTS ; NEURAL STEM-CELLS ; PROGENITOR CELLS ; PIGMENT-EPITHELIUM ; PHOTORECEPTOR DEGENERATION ; SUBRETINAL FLUID ; IN-VITRO ; DIFFERENTIATION
英文摘要:

Aqueous humor has been shown to influence the proliferation of various ocular cell types, but the effect on immature retinal cells is not known. Here, the effect of pig aqueous humor on the proliferation of rat retinal precursor cells (RPCs) was investigated. RPCs were prepared from embryonic day 19 Sprague-Dawley rats and cultured in the presence or absence of aqueous humor from healthy pigs along with a medium consisting of Dulbecco′s modified Eagle′s medium: Ham′s F-12 medium, N2 supplement, and epidermal growth factor. Proliferation was quantified by [H-3] thymidine incorporation under different treatment conditions, and any associated morphological changes were noted. Potential active components of porcine aqueous humor were partially characterized by gel filtration chromatography, and the effect on RPC proliferation was determined. Results showed that adding 20% aqueous humor increased [H-3] thymidine incorporation by as much as 317%, as compared with controls. Aqueous supplementation also increased both the number and size of RPC spherical aggregates ("spheres") over the first 4 days, consistent with increased proliferative activity. Using gel filtration and the in vitro proliferation assay, the growth- promoting activity of aqueous humor was localized to two different molecular mass ranges, namely, around 30 kDa and less than 1 kDa. Ascorbic acid was present in the lower molecular mass fraction, and use of this molecule reproduced some, but not all, of the proliferative activity present in aqueous humor. These results highlight the potential role of soluble factors present in the cellular microenvironment with respect to modulation of endogenous progenitor cell activity.

语种: 英语
WOS记录号: WOS:000242696200018
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55967
Appears in Collections:北京大学第三临床医学院_眼科_期刊论文

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作者单位: 1.Univ Copenhagen, Panum Inst, Dept Med Anat, Expt Immunol Lab, DK-2200 Copenhagen, Denmark
2.Peking Univ, Hosp 3, Dept Ophthalmol, Beijing 100871, Peoples R China
3.Singapore Eye Res Inst, Singapore, Singapore

Recommended Citation:
Yang, Jing,Klassen, Henry,Pries, Mette,et al. Aqueous humor enhances the proliferation of rat retinal precursor cells in culture, and this effect is partially reproduced by ascorbic acid[J]. STEM CELLS,2006,24(12):2766-2775.
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