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IR@PKUHSC  > 北京大学临床肿瘤学院  > 泌尿外科  > 期刊论文
学科主题: 临床医学
题名:
Expression pattern of REIC/Dkk-3 in various cell types and the implications of the soluble form in prostatic acinar development
作者: Zhang, Kai2; Watanabe, Masami1,2,3; Kashiwakura, Yuji2; Li, Shun-Al2,3; Edamura, Kohei1; Huang, Peng2,3; Yamaguchi, Ken1,3; Nasu, Yasutomo1; Kobayashi, Yasuyuki1; Sakaguchi, Masakiyo4; Ochiai, Kazuhiko2; Yamada, Hiroshi5; Takei, Kohji5; Ueki, Hideo1; Huh, Nam-Ho4; Li, Ming6; Kaku, Haruki1,2,3; Na, Yanqun7; Kumon, Hiromi1,2,3
关键词: REIC/Dkk-3 ; tissue distribution ; subcellular localization ; prostate actinar development
刊名: INTERNATIONAL JOURNAL OF ONCOLOGY
发表日期: 2010-12-01
DOI: 10.3892/ijo_00000802
卷: 37, 期:6, 页:1495-1501
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: TUMOR-GROWTH ; DOWN-REGULATION ; CANCER ; APOPTOSIS ; GENE ; OVEREXPRESSION ; DICKKOPF-3 ; SUPPRESSES ; ACTIVATION ; INDUCTION
英文摘要:

The tumor suppressor REIC/Dkk-3 is a secretory protein which was originally identified to be downregulated in human immortalized cells In the present study, we investigated the expression pattern of REIC/Dkk-3 in various cell types to characterize its physiological functions We first examined the expression level of REIC/Dkk-3 in a broad range of cancer cell types and confirmed that it was significantly downregulated in all of the cell types We also examined the tissue distribution pattern in a variety of normal mouse organs Ubiquitous REIC/Dkk-3 protein expression was observed in the organs The expression was abundant in the liver, heart and brain tissue, but was absent in the spleen and peripheral blood mononuclear cells The immunohistochemical analyses revealed that the subcellular localization of REIC/Dkk-3 had a punctate pattern around the nucleus, indicating its association with secretory vesicles In cancer cells stably transfected with REIC/Dkk-3 the protein was predominantly localized to the endoplasmic reticulum (ER) under observation with confocal microscopy Because REIC/ Dkk-3 was found to be abundantly expressed in the acinar epithelial cells of the mouse prostate, we analyzed the effects of recombinant REIC/Dkk-3 protein on the acinar morphogenesis of RWPE-1 cells, which are derived from human normal prostate epithelium Statistically significant acinar growth was observed in the culture condition with 10 mu g/m1 REIC/Dkk-3 protein, implicating the soluble form m prostatic acinar development Current results suggest that REIC/Dkk-3 may play a role in regulating the morphological process of normal tissue architecture through an autocrine and/or paracrine manner

语种: 英语
所属项目编号: FY2006
项目资助者: Ministry of Education, Culture, Sports, Science and Technology
WOS记录号: WOS:000284922700015
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55980
Appears in Collections:北京大学临床肿瘤学院_泌尿外科_期刊论文

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作者单位: 1.Okayama Univ, Dept Urol, Grad Sch Med Dent & Pharmaceut Sci, Okayama 7008558, Japan
2.Okayama Univ, Innovat Ctr Okayama Nanobiotargeted Therapy, Grad Sch Med Dent & Pharmaceut Sci, Okayama 7008558, Japan
3.Okayama Univ, Ctr Gene & Cell Therapy, Grad Sch Med Dent & Pharmaceut Sci, Okayama 7008558, Japan
4.Okayama Univ, Dept Cell Biol, Grad Sch Med Dent & Pharmaceut Sci, Okayama 7008558, Japan
5.Peking Univ, ShouGang Hosp, Dept Urol, Beijing, Peoples R China
6.Okayama Univ, Dept Neurosci, Grad Sch Med Dent & Pharmaceut Sci, Okayama 7008558, Japan
7.Peking Univ, Sch Oncol, Dept Urol, Beijing Canc Hosp & Inst, Beijing, Peoples R China

Recommended Citation:
Zhang, Kai,Watanabe, Masami,Kashiwakura, Yuji,et al. Expression pattern of REIC/Dkk-3 in various cell types and the implications of the soluble form in prostatic acinar development[J]. INTERNATIONAL JOURNAL OF ONCOLOGY,2010,37(6):1495-1501.
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