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学科主题临床医学
Expression pattern of REIC/Dkk-3 in various cell types and the implications of the soluble form in prostatic acinar development
Zhang, Kai2; Watanabe, Masami1,2,3; Kashiwakura, Yuji2; Li, Shun-Al2,3; Edamura, Kohei1; Huang, Peng2,3; Yamaguchi, Ken1,3; Nasu, Yasutomo1; Kobayashi, Yasuyuki1; Sakaguchi, Masakiyo4; Ochiai, Kazuhiko2; Yamada, Hiroshi5; Takei, Kohji5; Ueki, Hideo1; Huh, Nam-Ho4; Li, Ming6; Kaku, Haruki1,2,3; Na, Yanqun7; Kumon, Hiromi1,2,3
关键词Reic/dkk-3 Tissue Distribution Subcellular Localization Prostate Actinar Development
刊名INTERNATIONAL JOURNAL OF ONCOLOGY
2010-12-01
DOI10.3892/ijo_00000802
37期:6页:1495-1501
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
研究领域[WOS]Oncology
关键词[WOS]TUMOR-GROWTH ; DOWN-REGULATION ; CANCER ; APOPTOSIS ; GENE ; OVEREXPRESSION ; DICKKOPF-3 ; SUPPRESSES ; ACTIVATION ; INDUCTION
英文摘要

The tumor suppressor REIC/Dkk-3 is a secretory protein which was originally identified to be downregulated in human immortalized cells In the present study, we investigated the expression pattern of REIC/Dkk-3 in various cell types to characterize its physiological functions We first examined the expression level of REIC/Dkk-3 in a broad range of cancer cell types and confirmed that it was significantly downregulated in all of the cell types We also examined the tissue distribution pattern in a variety of normal mouse organs Ubiquitous REIC/Dkk-3 protein expression was observed in the organs The expression was abundant in the liver, heart and brain tissue, but was absent in the spleen and peripheral blood mononuclear cells The immunohistochemical analyses revealed that the subcellular localization of REIC/Dkk-3 had a punctate pattern around the nucleus, indicating its association with secretory vesicles In cancer cells stably transfected with REIC/Dkk-3 the protein was predominantly localized to the endoplasmic reticulum (ER) under observation with confocal microscopy Because REIC/ Dkk-3 was found to be abundantly expressed in the acinar epithelial cells of the mouse prostate, we analyzed the effects of recombinant REIC/Dkk-3 protein on the acinar morphogenesis of RWPE-1 cells, which are derived from human normal prostate epithelium Statistically significant acinar growth was observed in the culture condition with 10 mu g/m1 REIC/Dkk-3 protein, implicating the soluble form m prostatic acinar development Current results suggest that REIC/Dkk-3 may play a role in regulating the morphological process of normal tissue architecture through an autocrine and/or paracrine manner

语种英语
WOS记录号WOS:000284922700015
项目编号FY2006
资助机构Ministry of Education, Culture, Sports, Science and Technology
引用统计
被引频次:26[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/55980
专题北京大学临床肿瘤学院_泌尿外科
作者单位1.Okayama Univ, Dept Urol, Grad Sch Med Dent & Pharmaceut Sci, Okayama 7008558, Japan
2.Okayama Univ, Innovat Ctr Okayama Nanobiotargeted Therapy, Grad Sch Med Dent & Pharmaceut Sci, Okayama 7008558, Japan
3.Okayama Univ, Ctr Gene & Cell Therapy, Grad Sch Med Dent & Pharmaceut Sci, Okayama 7008558, Japan
4.Okayama Univ, Dept Cell Biol, Grad Sch Med Dent & Pharmaceut Sci, Okayama 7008558, Japan
5.Peking Univ, ShouGang Hosp, Dept Urol, Beijing, Peoples R China
6.Okayama Univ, Dept Neurosci, Grad Sch Med Dent & Pharmaceut Sci, Okayama 7008558, Japan
7.Peking Univ, Sch Oncol, Dept Urol, Beijing Canc Hosp & Inst, Beijing, Peoples R China
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Zhang, Kai,Watanabe, Masami,Kashiwakura, Yuji,et al. Expression pattern of REIC/Dkk-3 in various cell types and the implications of the soluble form in prostatic acinar development[J]. INTERNATIONAL JOURNAL OF ONCOLOGY,2010,37(6):1495-1501.
APA Zhang, Kai.,Watanabe, Masami.,Kashiwakura, Yuji.,Li, Shun-Al.,Edamura, Kohei.,...&Kumon, Hiromi.(2010).Expression pattern of REIC/Dkk-3 in various cell types and the implications of the soluble form in prostatic acinar development.INTERNATIONAL JOURNAL OF ONCOLOGY,37(6),1495-1501.
MLA Zhang, Kai,et al."Expression pattern of REIC/Dkk-3 in various cell types and the implications of the soluble form in prostatic acinar development".INTERNATIONAL JOURNAL OF ONCOLOGY 37.6(2010):1495-1501.
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