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学科主题基础医学
Intracellular Proton-mediated Activation of TRPV3 Channels Accounts for the Exfoliation Effect of alpha-Hydroxyl Acids on Keratinocytes
Cao, Xu1,2; Yang, Fan1; Zheng, Jie1; Wang, Kewei2,3
刊名JOURNAL OF BIOLOGICAL CHEMISTRY
2012-07-27
DOI10.1074/jbc.M112.364869
287期:31页:25905-25916
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]RECEPTOR POTENTIAL CHANNELS ; CAPSAICIN RECEPTOR ; 2-AMINOETHOXYDIPHENYL BORATE ; GLYCOLIC ACID ; ION CHANNELS ; NOCICEPTIVE NEURONS ; COMMON ACTIVATOR ; CHORDA TYMPANI ; CATION CHANNEL ; HAIR-GROWTH
英文摘要

alpha-Hydroxyl acids (AHAs) from natural sources act as proton donors and topical compounds that penetrate skin and are well known in the cosmetic industry for their use in chemical peels and improvement of the skin. However, little is known about how AHAs cause exfoliation to expose fresh skin cells. Here we report that the transient receptor potential vanilloid 3 (TRPV3) channel in keratinocytes is potently activated by intracellular acidification induced by glycolic acid. Patch clamp recordings and cell death assay of both human keratinocyte HaCaT cells and TRPV3-expressing HEK-293 cells confirmed that intracellular acidification led to direct activation of TRPV3 and promoted cell death. Site-directed mutagenesis revealed that an N-terminal histidine residue, His-426, known to be involved in 2-aminoethyl diphenylborinate-mediated TRPV3 activation, is critical for sensing intracellular proton levels. Taken together, our findings suggest that intracellular protons can strongly activate TRPV3, and TRPV3-mediated proton sensing and cell death in keratinocytes may serve as a molecular basis for the cosmetic use of AHAs and their therapeutic potential in acidic pH-related skin disorders.

语种英语
WOS记录号WOS:000306916300019
项目编号R01NS072377 ; 30970919 ; B07001
资助机构National Institutes of Health ; National Science Foundation of China ; Ministry of Education of China 111 Project China ; China Scholarship Council
引用统计
被引频次:26[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/55981
专题北京大学基础医学院_神经生物学系
北京大学药学院_分子与细胞药理学系
作者单位1.Univ Calif Davis, Sch Med, Dept Physiol & Membrane Biol, Davis, CA 95616 USA
2.Peking Univ, Hlth Sci Ctr, Dept Neurobiol, Neurosci Res Inst, Beijing 100191, Peoples R China
3.Peking Univ, Dept Mol & Cellular Pharmacol, State Key Lab Nat & Biomimet Drugs, Sch Pharmaceut Sci, Beijing 100191, Peoples R China
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GB/T 7714
Cao, Xu,Yang, Fan,Zheng, Jie,et al. Intracellular Proton-mediated Activation of TRPV3 Channels Accounts for the Exfoliation Effect of alpha-Hydroxyl Acids on Keratinocytes[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2012,287(31):25905-25916.
APA Cao, Xu,Yang, Fan,Zheng, Jie,&Wang, Kewei.(2012).Intracellular Proton-mediated Activation of TRPV3 Channels Accounts for the Exfoliation Effect of alpha-Hydroxyl Acids on Keratinocytes.JOURNAL OF BIOLOGICAL CHEMISTRY,287(31),25905-25916.
MLA Cao, Xu,et al."Intracellular Proton-mediated Activation of TRPV3 Channels Accounts for the Exfoliation Effect of alpha-Hydroxyl Acids on Keratinocytes".JOURNAL OF BIOLOGICAL CHEMISTRY 287.31(2012):25905-25916.
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