北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学药学院  > 药剂学系  > 期刊论文
学科主题: 药学
题名:
A novel stealth liposomal topotecan with amlodipine: Apoptotic effect is associated with deletion of intracellular Ca2+ by amlodipine thus leading to an enhanced antitumor activity in leukemia
作者: Li, X; Ruan, GR; Lu, WL; Hong, HY; Liang, GW; Zhang, YT; Liu, Y; Long, C; Ma, X; Yuan, L; Wang, JC; Zhang, X; Zhang, Q
关键词: stealth liposomal topotecan with amlodipine ; caspases ; intracellular calcium ion ; signaling pathway ; leukemia
刊名: JOURNAL OF CONTROLLED RELEASE
发表日期: 2006-05-15
DOI: 10.1016/j.jconrel.2006.01.007
卷: 112, 期:2, 页:186-198
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
研究领域[WOS]: Chemistry ; Pharmacology & Pharmacy
关键词[WOS]: CELL LUNG-CANCER ; CARCINOMA A431 CELLS ; IN-VITRO ; OVARIAN-CANCER ; BREAST-CANCER ; RESISTANCE PROTEIN ; PHASE-I ; DOXORUBICIN ; GROWTH ; MICE
英文摘要:

The objectives of the present study were to define whether amlodipine induces apoptosis and what mechanism is involved in the process in human resistant and non-resistant leukemia cells following co-administration of stealth liposomal topotecan with amlodipine, a novel antiresistant liposomes developed by our institution. In three leukemias, K562, HL-60, and multidrug resistant (MDR) HL-60, cytotoxicity of topotecan was potentiated by amolodipne, while topotecan alone was resistant to MDR HL-60 cells. In two selected K562 or MDR HL-60 cells, the apoptotic effects were increased by addition of amlodipme, showing a dose-dependent manner. The activities of caspase 3 and 7 (marked as caspase 3/7), and caspase 8 were significantly activated by topotecan with amlodipme co-treated as the stealth liposomes. The deletions of intracellular Ca2+ stores induced by amlodipine correlated with the activated activities of caspase 3/7, or 8, respectively. In xenograft model with MDR HL-60 in nude mice, antitumor activity of stealth liposomal topotecan with amlodipine was significantly enhanced as compared to that of stealth liposomal topotecan or topotecan alone. In conclusion, apoptotic effect is associated with deletion of intracellular Ca2+ by amlodipine through activation of caspase 8 and then 3/7 activities. The enhanced antitumor activities by stealth liposomal topotecan with amlodipine are mainly due to the potentiating apoptotic effect and reversing the resistance by amlodipine. Stealth liposomal encapsulation of anticancer agent with a modulator may provide a novel strategy for improving the chemotherapeutic effects. (c) 2006 Elsevier B.V. All rights reserved.

语种: 英语
WOS记录号: WOS:000237908100005
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55983
Appears in Collections:北京大学药学院_药剂学系_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Peking Univ, Peoples Hosp, Beijing 100083, Peoples R China
2.Peking Univ, Inst Hematol, Beijing 100083, Peoples R China
3.Peking Univ, Sch Pharmaceut Sci, Dept Pharmaceut, Beijing 100083, Peoples R China
4.Beijing Med Univ, United Biol Engn Co, Beijing 100083, Peoples R China
5.Peking Univ, Ctr Human Dis Genom, Beijing 100083, Peoples R China

Recommended Citation:
Li, X,Ruan, GR,Lu, WL,et al. A novel stealth liposomal topotecan with amlodipine: Apoptotic effect is associated with deletion of intracellular Ca2+ by amlodipine thus leading to an enhanced antitumor activity in leukemia[J]. JOURNAL OF CONTROLLED RELEASE,2006,112(2):186-198.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Li, X]'s Articles
[Ruan, GR]'s Articles
[Lu, WL]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Li, X]‘s Articles
[Ruan, GR]‘s Articles
[Lu, WL]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace