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学科主题: 药学
题名:
Lanthanum Suppresses Osteoblastic Differentiation Via Pertussis Toxin-Sensitive G Protein Signaling in Rat Vascular Smooth Muscle Cells
作者: Shi, Yan-Ling2; Wang, Li-Wen2; Huang, Jian1; Gou, Bao-Di1; Zhang, Tian-Lan1,2; Wang, Kui1
关键词: VASCULAR SMOOTH MUSCLE CELL ; OSTEOBLASTIC DIFFERENTIATION ; LANTHANUM ; G PROTEIN ; MAPK PATHWAYS
刊名: JOURNAL OF CELLULAR BIOCHEMISTRY
发表日期: 2009-12-01
DOI: 10.1002/jcb.22348
卷: 108, 期:5, 页:1184-1191
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Cell Biology
研究领域[WOS]: Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]: CALCIUM-SENSING RECEPTOR ; ARTERIAL CALCIFICATION ; BETA-GLYCEROPHOSPHATE ; BONE-FORMATION ; PROLIFERATION ; ERK ; ATHEROSCLEROSIS ; CYTOTOXICITY ; INHIBITION ; ACTIVATION
英文摘要:

A major cellular event in vascular calcification is the phenotypic transformation of vascular smooth muscle cells (VSMCs) into osteoblast-like cells. After demonstrating that lanthanum chloride (LaCl(3)) suppresses hydrogen peroxide-enhanced calcification in rat calcifying vascular cells (CVCs), here we report its effect on the osteoblastic differentiation of rat VSMCs, a process leading to the formation of CVCs. Cells were isolated from aortic media of male SD rats, and passages between three and eight were cultured in Dulbeccol′s Modified Eagle′s Medium (DMEM) containing 10% fetal bovine serum (FBS) and 10 mM beta-glycerophosphate (beta-GP) in the presence or absence of LaCl(3). Exposure of cells to LaCl(3) suppressed the P-GP-induced elevations in calcium deposition, alkaline phosphatase (ALP) activity, and Cbfa1/Runx2 expression, as well as the concomitant loss of SM alpha-actin. Furthermore, LaCl(3) activated the phosphorylation of extracellular signal-regulated kinase (ERK) and c-Jun N-terminal kinase (JNK), and the blockage of either pathway with a specific inhibitor abolished the effects of LaCl(3). In addition, pretreatment of the cells with pertussis toxin (PTx), an inhibitor of G protein-mediated signaling pathway, repealed all the changes induced by LaCl(3). These findings demonstrate that LaCl(3) suppresses the P-GP-induced osteoblastic differentiation and calcification in rat VSMCs, and its effect is mediated by the activation of both ERK and JNK MAPK pathways via PTx-sensitive G proteins. J. Cell. Biochem. 108: 1184-1191, 2009. (C) 2009 Wiley-Liss, Inc.

语种: 英语
WOS记录号: WOS:000272640900018
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55990
Appears in Collections:北京大学药学院_化学生物学系_期刊论文

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作者单位: 1.Peking Univ, Sch Pharmaceut Sci, Dept Biol Chem, Beijing 100191, Peoples R China
2.Capital Med Univ, Dept Biol Chem, Sch Pharmaceut Sci, Beijing 100069, Peoples R China

Recommended Citation:
Shi, Yan-Ling,Wang, Li-Wen,Huang, Jian,et al. Lanthanum Suppresses Osteoblastic Differentiation Via Pertussis Toxin-Sensitive G Protein Signaling in Rat Vascular Smooth Muscle Cells[J]. JOURNAL OF CELLULAR BIOCHEMISTRY,2009,108(5):1184-1191.
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