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Cox7a2 mediates steroidogenesis in TM3 mouse Leydig cells
Chen, Liang; Xin, Zhong-Cheng; Li, Xin; Tian, Long; Yuan, Yi-Ming; Liu, Gang; Jiang, Xue-Jun; Guo, Ying-Lu
关键词Cox7a2 Fusion Protein Steroidogenesis Steroidogenic Acute Regulatory Protein Reactive Oxygen Species Leydig Cell
刊名ASIAN JOURNAL OF ANDROLOGY
2006-09-01
DOI10.1111/j.1745-7262.2006.00178.x
8期:5页:589-594
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Andrology ; Urology & Nephrology
研究领域[WOS]Endocrinology & Metabolism ; Urology & Nephrology
关键词[WOS]ACUTE REGULATORY PROTEIN ; CHOLESTEROL ; STAR ; RAT ; MITOCHONDRIA ; EXPRESSION ; LINES
英文摘要

Aim: To investigate the regulatory function of Cox7a2 on steroidogenesis and the mechanism involved in TM3 mouse Leydig cells. Methods: The cDNA of Cox7a2 was cloned from TM3 mouse Leydig cells. It was subcloned to pDsRed-Express-N1 and transfected back into TM3 mouse Leydig cells for Cox7a2 overexpression by transient gene transfection. Steroidogenesis affected by overexpressed Cox7a2 was studied by ELISA. To elicit the mechanism of this effect, expression of steroidogenic acute regulatory (StAR) protein and reactive oxygen species (ROS) were examined by Western blot and fluorometer, respectively. Results: The cDNA of Cox7a2 (249 bp) was cloned from Leydig cells and confirmed by DNA sequencing. After constructed pDsRed-Express-N1-Cox7a2 was transfected back into TM3 mouse Leydig cells, Cox7a2 inhibited not only luteinizing hormone (LH)-induced secretion of testosterone but also the expression of StAR protein. At the same time, Cox7a2 increased the activity of ROS in TM3 mouse Leydig cells. Conclusion: Cox7a2 inhibited LH-induced StAR protein expression, and consequent testosterone production, at least in part, by increasing ROS activity in TM3 mouse Leydig cells.

语种英语
WOS记录号WOS:000239861400012
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/55996
专题北京大学第一临床医学院_男科中心
作者单位1.Peking Univ, Hosp 1, Androl Ctr, Beijing 100093, Peoples R China
2.Peoples Hosp Zhangqiu, Dept Surg, Zhangqiu 250200, Peoples R China
3.Chinese Acad Sci, Inst Microbiol, Beijing 100080, Peoples R China
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Chen, Liang,Xin, Zhong-Cheng,Li, Xin,et al. Cox7a2 mediates steroidogenesis in TM3 mouse Leydig cells[J]. ASIAN JOURNAL OF ANDROLOGY,2006,8(5):589-594.
APA Chen, Liang.,Xin, Zhong-Cheng.,Li, Xin.,Tian, Long.,Yuan, Yi-Ming.,...&Guo, Ying-Lu.(2006).Cox7a2 mediates steroidogenesis in TM3 mouse Leydig cells.ASIAN JOURNAL OF ANDROLOGY,8(5),589-594.
MLA Chen, Liang,et al."Cox7a2 mediates steroidogenesis in TM3 mouse Leydig cells".ASIAN JOURNAL OF ANDROLOGY 8.5(2006):589-594.
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