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学科主题: 药学
题名:
Liver X receptor alpha mediated genistein induction of human dehydroepiandrosterone sulfotransferase (hSULT2A1) in Hep G2 cells
作者: Chen, Yue1; Zhang, Shunfen1; Zhou, Tianyan2; Huang, Chaoqun1; McLaughlin, Alicia1; Chen, Guangping1
关键词: Sulfotransferase ; Genistein ; Liver X receptor ; SULT2A1 ; Gene regulation
刊名: TOXICOLOGY AND APPLIED PHARMACOLOGY
发表日期: 2013-04-15
DOI: 10.1016/j.taap.2013.01.006
卷: 268, 期:2, 页:106-112
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Pharmacology & Pharmacy ; Toxicology
研究领域[WOS]: Pharmacology & Pharmacy ; Toxicology
关键词[WOS]: CONSTITUTIVE ANDROSTANE RECEPTOR ; ARYL-HYDROCARBON RECEPTOR ; METHOTREXATE INDUCTION ; ACID-SULFOTRANSFERASE ; NUCLEAR RECEPTORS ; SOY ISOFLAVONES ; HUMAN BREAST ; SULFATE CONJUGATION ; GENE-EXPRESSION ; GROWTH
英文摘要:

Cytosolic sulfotransferases are one of the major families of phase II drug metabolizing enzymes. Sulfotransferase-catalyzed sulfonation regulates hormone activities, metabolizes drugs, detoxifies xenobiotics, and bioactivates carcinogens. Human dehydroepiandrosterone sulfotransferase (hSULT2A1) plays important biological roles by sulfating endogenous hydroxysteroids and exogenous xenobiotics. Genistein, mainly existing in soy food products, is a naturally occurring phytoestrogen with both chemopreventive and chemotherapeutic potential. Our previous studies have shown that genistein significantly induces hSULT2A1 in Hep G2 and Caco-2 cells. In this study, we investigated the roles of liver X receptor (LXR alpha) in the genistein induction of hSULT2A1. LXRs have been shown to induce expression of mouse Sult2a9 and hSULT2A1 gene. Our results demonstrate that LXRa mediates the genistein induction of hSULT2A1, supported by Western blot analysis results, hSULT2A1 promoter driven luciferase reporter gene assay results, and mRNA interference results. Chromatin immunoprecipitation (ChIP) assay results demonstrate that genistein increase the recruitment of hIXR alpha binding to the hSULT2A1 promoter. These results suggest that hLXR alpha plays an important role in the hSULT2A1 gene regulation. The biological functions of phytoestrogens may partially relate to their induction activity toward hydroxysteroid SULT. (C) 2013 Elsevier Inc. All rights reserved.

语种: 英语
所属项目编号: GM078606 ; RSG-07-028-01-CNE ; 2006-35200-17137 ; HR05-015
项目资助者: NIH ; American Cancer Society ; USDA ; Oklahoma Center for the Advancement of Science and Technology (OCAST)
WOS记录号: WOS:000316978700002
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56013
Appears in Collections:北京大学药学院_药剂学系_期刊论文

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作者单位: 1.Oklahoma State Univ, Dept Physiol Sci, Ctr Vet Hlth Sci, Stillwater, OK 74078 USA
2.Peking Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, Dept Pharmaceut, Beijing 100083, Peoples R China

Recommended Citation:
Chen, Yue,Zhang, Shunfen,Zhou, Tianyan,et al. Liver X receptor alpha mediated genistein induction of human dehydroepiandrosterone sulfotransferase (hSULT2A1) in Hep G2 cells[J]. TOXICOLOGY AND APPLIED PHARMACOLOGY,2013,268(2):106-112.
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