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Liver X receptor alpha mediated genistein induction of human dehydroepiandrosterone sulfotransferase (hSULT2A1) in Hep G2 cells
Chen, Yue1; Zhang, Shunfen1; Zhou, Tianyan2; Huang, Chaoqun1; McLaughlin, Alicia1; Chen, Guangping1
关键词Sulfotransferase Genistein Liver x Receptor Sult2a1 Gene Regulation
刊名TOXICOLOGY AND APPLIED PHARMACOLOGY
2013-04-15
DOI10.1016/j.taap.2013.01.006
268期:2页:106-112
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Pharmacology & Pharmacy ; Toxicology
研究领域[WOS]Pharmacology & Pharmacy ; Toxicology
关键词[WOS]CONSTITUTIVE ANDROSTANE RECEPTOR ; ARYL-HYDROCARBON RECEPTOR ; METHOTREXATE INDUCTION ; ACID-SULFOTRANSFERASE ; NUCLEAR RECEPTORS ; SOY ISOFLAVONES ; HUMAN BREAST ; SULFATE CONJUGATION ; GENE-EXPRESSION ; GROWTH
英文摘要

Cytosolic sulfotransferases are one of the major families of phase II drug metabolizing enzymes. Sulfotransferase-catalyzed sulfonation regulates hormone activities, metabolizes drugs, detoxifies xenobiotics, and bioactivates carcinogens. Human dehydroepiandrosterone sulfotransferase (hSULT2A1) plays important biological roles by sulfating endogenous hydroxysteroids and exogenous xenobiotics. Genistein, mainly existing in soy food products, is a naturally occurring phytoestrogen with both chemopreventive and chemotherapeutic potential. Our previous studies have shown that genistein significantly induces hSULT2A1 in Hep G2 and Caco-2 cells. In this study, we investigated the roles of liver X receptor (LXR alpha) in the genistein induction of hSULT2A1. LXRs have been shown to induce expression of mouse Sult2a9 and hSULT2A1 gene. Our results demonstrate that LXRa mediates the genistein induction of hSULT2A1, supported by Western blot analysis results, hSULT2A1 promoter driven luciferase reporter gene assay results, and mRNA interference results. Chromatin immunoprecipitation (ChIP) assay results demonstrate that genistein increase the recruitment of hIXR alpha binding to the hSULT2A1 promoter. These results suggest that hLXR alpha plays an important role in the hSULT2A1 gene regulation. The biological functions of phytoestrogens may partially relate to their induction activity toward hydroxysteroid SULT. (C) 2013 Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000316978700002
项目编号GM078606 ; RSG-07-028-01-CNE ; 2006-35200-17137 ; HR05-015
资助机构NIH ; American Cancer Society ; USDA ; Oklahoma Center for the Advancement of Science and Technology (OCAST)
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56013
专题北京大学药学院_药剂学系
作者单位1.Oklahoma State Univ, Dept Physiol Sci, Ctr Vet Hlth Sci, Stillwater, OK 74078 USA
2.Peking Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, Dept Pharmaceut, Beijing 100083, Peoples R China
推荐引用方式
GB/T 7714
Chen, Yue,Zhang, Shunfen,Zhou, Tianyan,et al. Liver X receptor alpha mediated genistein induction of human dehydroepiandrosterone sulfotransferase (hSULT2A1) in Hep G2 cells[J]. TOXICOLOGY AND APPLIED PHARMACOLOGY,2013,268(2):106-112.
APA Chen, Yue,Zhang, Shunfen,Zhou, Tianyan,Huang, Chaoqun,McLaughlin, Alicia,&Chen, Guangping.(2013).Liver X receptor alpha mediated genistein induction of human dehydroepiandrosterone sulfotransferase (hSULT2A1) in Hep G2 cells.TOXICOLOGY AND APPLIED PHARMACOLOGY,268(2),106-112.
MLA Chen, Yue,et al."Liver X receptor alpha mediated genistein induction of human dehydroepiandrosterone sulfotransferase (hSULT2A1) in Hep G2 cells".TOXICOLOGY AND APPLIED PHARMACOLOGY 268.2(2013):106-112.
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