|G-protein Coupled Receptor 34 Knockdown Impairs the Proliferation and Migration of HGC-27 Gastric Cancer Cells In Vitro|
|Jin, Zhong-Tian1; Li, Kun2; Li, Mei3; Ren, Zhi-Gang4; Wang, Fu-Shun1; Zhu, Ji-Ye1; Leng, Xi-Sheng1; Yu, Wei-Dong2,3|
|关键词||Gastric Cancer Gpr34 Knockdown Migration Proliferation|
|刊名||CHINESE MEDICAL JOURNAL|
|WOS标题词||Science & Technology|
|类目[WOS]||Medicine, General & Internal|
|研究领域[WOS]||General & Internal Medicine|
|关键词[WOS]||UP-REGULATION ; GPR34 ; METASTASIS ; APOPTOSIS ; LYMPHOMA ; INVASION ; TARGET ; PIK3CA ; GROWTH|
Background: Overexpression of G-protein coupled receptor 34 (GPR34) affects the progression and prognosis of human gastric adenocarcinoma, however, the role of GPR34 in gastric cancer development and progression has not been well-determined. The current study aimed to investigate the effect of GPR34 knockdown on the proliferation, migration, and apoptosis of HGC-27 gastric cancer cells and the underlying mechanisms.
Methods: The expression of GPR34 in gastric cancer cell line HGC-27 was detected by quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting. HGC-27 cells were employed to construct the stable GPR34 knockdown cell model in this study. Real-time RT-PCR and Western blotting were applied to validate the effect of short hairpin RNA (ShRNA) on the expression of GPR34 in HGC-27 gastric cells. The proliferation, migration of these cells were examined by Cell Counting Kit-8 and transwell. We also measured expression profile of PI3K/PDK1/AKT and ERK using Western blotting.
Results: The ShRNA directed against GPR34 effectively inhibited both endogenous mRNA and protein expression levels of GPR34, and significantly down-regulated the expression of PIK3CB (P < 0.01), PIK3CD (P < 0.01), PDK1 (P < 0.01), phosphorylation of PDK1 (P < 0.01), Akt (P < 0.01), and ERK (P < 0.01). Furthermore, GPR34 knockdown resulted in an obvious reduction in HGC-27 cancer cell proliferation and migration activity (P < 0.01).
Conclusions: GPR34 knockdown impairs the proliferation and migration of HGC-27 gastric cancer cells in vitro and provides a potential implication for therapy of gastric cancer.
|项目编号||30872923 ; RDB2007-47 ; RDK2008-01 ; RDB2011-26|
|资助机构||National Natural Science Foundation of China ; Peking University People&prime ; s Hospital Research and Development Foundations|
|作者单位||1.Peking Univ, Dept Hepatobiliary Surg, Peoples Hosp, Beijing 100044, Peoples R China|
2.Peking Univ, Dept Gastroenterol, Peoples Hosp, Beijing 100044, Peoples R China
3.Peking Univ, Inst Clin Mol Biol, Peoples Hosp, Beijing 100044, Peoples R China
4.Beijing Changping Dist Hosp, Dept Gen Surg, Beijing 102200, Peoples R China
|Jin, Zhong-Tian,Li, Kun,Li, Mei,et al. G-protein Coupled Receptor 34 Knockdown Impairs the Proliferation and Migration of HGC-27 Gastric Cancer Cells In Vitro[J]. CHINESE MEDICAL JOURNAL,2015,128(4):545-549.|
|APA||Jin, Zhong-Tian.,Li, Kun.,Li, Mei.,Ren, Zhi-Gang.,Wang, Fu-Shun.,...&Yu, Wei-Dong.(2015).G-protein Coupled Receptor 34 Knockdown Impairs the Proliferation and Migration of HGC-27 Gastric Cancer Cells In Vitro.CHINESE MEDICAL JOURNAL,128(4),545-549.|
|MLA||Jin, Zhong-Tian,et al."G-protein Coupled Receptor 34 Knockdown Impairs the Proliferation and Migration of HGC-27 Gastric Cancer Cells In Vitro".CHINESE MEDICAL JOURNAL 128.4(2015):545-549.|