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学科主题: 临床医学
题名:
G-protein Coupled Receptor 34 Knockdown Impairs the Proliferation and Migration of HGC-27 Gastric Cancer Cells In Vitro
作者: Jin, Zhong-Tian1; Li, Kun2; Li, Mei3; Ren, Zhi-Gang4; Wang, Fu-Shun1; Zhu, Ji-Ye1; Leng, Xi-Sheng1; Yu, Wei-Dong2,3
关键词: Gastric Cancer ; GPR34 ; Knockdown ; Migration ; Proliferation
刊名: CHINESE MEDICAL JOURNAL
发表日期: 2015-02-20
DOI: 10.4103/0366-6999.151114
卷: 128, 期:4, 页:545-549
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, General & Internal
研究领域[WOS]: General & Internal Medicine
关键词[WOS]: UP-REGULATION ; GPR34 ; METASTASIS ; APOPTOSIS ; LYMPHOMA ; INVASION ; TARGET ; PIK3CA ; GROWTH
英文摘要:

Background: Overexpression of G-protein coupled receptor 34 (GPR34) affects the progression and prognosis of human gastric adenocarcinoma, however, the role of GPR34 in gastric cancer development and progression has not been well-determined. The current study aimed to investigate the effect of GPR34 knockdown on the proliferation, migration, and apoptosis of HGC-27 gastric cancer cells and the underlying mechanisms.

Methods: The expression of GPR34 in gastric cancer cell line HGC-27 was detected by quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting. HGC-27 cells were employed to construct the stable GPR34 knockdown cell model in this study. Real-time RT-PCR and Western blotting were applied to validate the effect of short hairpin RNA (ShRNA) on the expression of GPR34 in HGC-27 gastric cells. The proliferation, migration of these cells were examined by Cell Counting Kit-8 and transwell. We also measured expression profile of PI3K/PDK1/AKT and ERK using Western blotting.

Results: The ShRNA directed against GPR34 effectively inhibited both endogenous mRNA and protein expression levels of GPR34, and significantly down-regulated the expression of PIK3CB (P < 0.01), PIK3CD (P < 0.01), PDK1 (P < 0.01), phosphorylation of PDK1 (P < 0.01), Akt (P < 0.01), and ERK (P < 0.01). Furthermore, GPR34 knockdown resulted in an obvious reduction in HGC-27 cancer cell proliferation and migration activity (P < 0.01).

Conclusions: GPR34 knockdown impairs the proliferation and migration of HGC-27 gastric cancer cells in vitro and provides a potential implication for therapy of gastric cancer.

语种: 英语
所属项目编号: 30872923 ; RDB2007-47 ; RDK2008-01 ; RDB2011-26
项目资助者: National Natural Science Foundation of China ; Peking University People&prime ; s Hospital Research and Development Foundations
WOS记录号: WOS:000349523900022
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56015
Appears in Collections:北京大学第二临床医学院_肝胆外科_期刊论文

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作者单位: 1.Peking Univ, Dept Hepatobiliary Surg, Peoples Hosp, Beijing 100044, Peoples R China
2.Peking Univ, Dept Gastroenterol, Peoples Hosp, Beijing 100044, Peoples R China
3.Peking Univ, Inst Clin Mol Biol, Peoples Hosp, Beijing 100044, Peoples R China
4.Beijing Changping Dist Hosp, Dept Gen Surg, Beijing 102200, Peoples R China

Recommended Citation:
Jin, Zhong-Tian,Li, Kun,Li, Mei,et al. G-protein Coupled Receptor 34 Knockdown Impairs the Proliferation and Migration of HGC-27 Gastric Cancer Cells In Vitro[J]. CHINESE MEDICAL JOURNAL,2015,128(4):545-549.
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