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学科主题: 临床医学
题名:
Salting-out-assisted liquid-liquid extraction with acetonitrile for the determination of trimetazidine in rat plasma using liquid chromatography-mass spectrometry
作者: Xiong, Xin1,2; Yang, Li1
关键词: trimetazidine ; LC-MS/MS ; rat plasma ; SALLE
刊名: BIOMEDICAL CHROMATOGRAPHY
发表日期: 2015-02-01
DOI: 10.1002/bmc.3271
卷: 29, 期:2, 页:268-274
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical ; Pharmacology & Pharmacy
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy
关键词[WOS]: LIQUID/LIQUID EXTRACTION ; MS METHOD ; BIOEQUIVALENCE ; LC ; VALIDATION ; VOLUNTEERS
英文摘要:

A high-throughout bioanalytical method based on salting-out-assisted liquid/liquid extraction (SALLE) method with acetonitrile and mass spectrometry-compatible salts followed by LC-MS/MS analysis of trimetazidine in rat plasma is presented. It required only 50L of plasma and allows the use of minimal volumes of organic solvents. The seamless interface of SALLE and LC-MS eliminated the drying-down step and the extract was diluted and injected into an LC-MS/MS system with a cycle time of 2.5min/sample. The retention times of trimetazidine and IS were approximately 1.1 and 1.7min, respectively. Calibration curves were linear over the concentration range of 0.1-100ng/mL, which can be extended to 500ng/mL by dilution. The intra- and inter-batch precision, accuracy and the relative standard deviation were all <15%. This method was successfully applied to determine trimetazidine concentrations in rat plasma. Copyright (c) 2014 John Wiley & Sons, Ltd.

语种: 英语
WOS记录号: WOS:000347804700018
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56017
Appears in Collections:北京大学第三临床医学院_药剂科_期刊论文

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作者单位: 1.Peking Univ, Hosp 3, Dept Pharm, Beijing 100191, Peoples R China
2.Peking Univ, Therapeut Drug Monitoring & Clin Toxicol Ctr, Beijing 100191, Peoples R China

Recommended Citation:
Xiong, Xin,Yang, Li. Salting-out-assisted liquid-liquid extraction with acetonitrile for the determination of trimetazidine in rat plasma using liquid chromatography-mass spectrometry[J]. BIOMEDICAL CHROMATOGRAPHY,2015,29(2):268-274.
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