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A systematic analysis of the skeletal muscle miRNA transcriptome of chicken varieties with divergent skeletal muscle growth identifies novel miRNAs and differentially expressed miRNAs
Li, Tingting1,2,3; Wu, Rimao1; Zhang, Yong1; Zhu, Dahai1
刊名BMC GENOMICS
2011-04-13
DOI10.1186/1471-2164-12-186
12
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biotechnology & Applied Microbiology ; Genetics & Heredity
资助者National Basic Research Program of China ; National Natural Science Foundation of China ; Chinese National Programs for High Technology Research and Development ; National Basic Research Program of China ; National Natural Science Foundation of China ; Chinese National Programs for High Technology Research and Development
研究领域[WOS]Biotechnology & Applied Microbiology ; Genetics & Heredity
关键词[WOS]FACTOR-BETA SUPERFAMILY ; GENE-EXPRESSION ; MICRORNA TRANSCRIPTOME ; MIR-206 ; PROLIFERATION ; INHIBITION ; PATTERNS ; TARGETS ; CELLS ; MODEL
英文摘要

Background: Functional studies have demonstrated that microRNAs (miRNAs or miRs) play critical roles in a wide spectrum of biological processes including development and disease pathogenesis. To investigate the functional roles that miRNAs play during chicken skeletal muscle development, the miRNA transcriptomes of skeletal muscles from broiler and layer chickens were profiled using Solexa deep sequencing.

Results: Some miRNAs have multiple isoforms and several miRNAs* are present at higher levels than their corresponding miRNAs. Thirty three novel and 189 known chicken miRNAs were identified using computational approaches. Subsequent miRNA transcriptome comparisons and real-time PCR validation experiments revealed 17 miRNAs that were differentially expressed between broilers and layers, and a number of targets of these miRNAs have been implicated in myogenesis regulation. Using integrative miRNA target-prediction and network-analysis approaches an interaction network of differentially expressed and muscle-related miRNAs and their putative targets was constructed, and miRNAs that could contribute to the divergent muscle growth of broiler and layer chickens by targeting the ACVR2B gene were identified, which can causes dramatic increases in muscle mass.

Conclusions: The present study provides the first transcriptome profiling-based evaluation of miRNA function during skeletal muscle development in chicken. Systematic predictions aided the identification of potential miRNAs and their targets, which could contribute to divergent muscle growth in broiler and layer chickens. Furthermore, these predictions generated information that can be utilized in further research investigating the involvement of interaction networks, containing miRNAs and their targets, in the regulation of muscle development.

语种英语
所属项目编号2011CBA01104 ; 2007CB946903 ; 2009CB941602 ; 2009CB825403 ; 31030041 ; 91019010 ; 30871248 ; 60905014 ; 2006AA10A121 ; 2007AA02Z109
资助者National Basic Research Program of China ; National Natural Science Foundation of China ; Chinese National Programs for High Technology Research and Development ; National Basic Research Program of China ; National Natural Science Foundation of China ; Chinese National Programs for High Technology Research and Development
WOS记录号WOS:000291225600001
引用统计
被引频次:47[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56025
专题基础医学院_医学信息学系
作者单位1.Chinese Acad Med Sci, Peking Union Med Coll, Inst Basic Med Sci, Natl Lab Med Mol Biol,Sch Basic Med, Beijing 100730, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Dept Biomed Informat, Beijing 100191, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Inst Syst Biomed, Beijing 100191, Peoples R China
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GB/T 7714
Li, Tingting,Wu, Rimao,Zhang, Yong,et al. A systematic analysis of the skeletal muscle miRNA transcriptome of chicken varieties with divergent skeletal muscle growth identifies novel miRNAs and differentially expressed miRNAs[J]. BMC GENOMICS,2011,12.
APA Li, Tingting,Wu, Rimao,Zhang, Yong,&Zhu, Dahai.(2011).A systematic analysis of the skeletal muscle miRNA transcriptome of chicken varieties with divergent skeletal muscle growth identifies novel miRNAs and differentially expressed miRNAs.BMC GENOMICS,12.
MLA Li, Tingting,et al."A systematic analysis of the skeletal muscle miRNA transcriptome of chicken varieties with divergent skeletal muscle growth identifies novel miRNAs and differentially expressed miRNAs".BMC GENOMICS 12(2011).
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