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学科主题: 基础医学
题名:
Activating transcription factor 4 is involved in endoplasmic reticulum stress-mediated apoptosis contributing to vascular calcification
作者: Duan, Xiao-Hui1; Chang, Jin-Rui2,3; Zhang, Jing4; Zhang, Bao-Hong5; Li, Yu-Lin1; Teng, Xu1; Zhu, Yi2,3; Du, Jie1; Tang, Chao-Shu1,2,3; Qi, Yong-Fen1,3
关键词: Activating transcription factor 4 ; Endoplasmic reticulum stress ; Apoptosis ; Vascular calcification
刊名: APOPTOSIS
发表日期: 2013-09-01
DOI: 10.1007/s10495-013-0861-3
卷: 18, 期:9, 页:1132-1144
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Cell Biology
研究领域[WOS]: Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]: UNFOLDED PROTEIN RESPONSE ; SMOOTH-MUSCLE-CELLS ; CHEMICAL CHAPERONES ; TAURINE PREVENTS ; MODEL ; MECHANISMS ; EXPRESSION
英文摘要:

Our previous work reported that endoplasmic reticulum stress (ERS)-mediated apoptosis was activated during vascular calcification (VC). Activating transcription factor 4 (ATF4) is a critical transcription factor in osteoblastogenesis and ERS-induced apoptosis. However, whether ATF4 is involved in ERS-mediated apoptosis contributing to VC remains unclear. In the present study, in vivo VC was induced in rats by administering vitamin D-3 plus nicotine. Vascular smooth muscle cell (VSMC) calcification in vitro was induced by incubation in calcifying media containing beta-glycerophosphate and CaCl2. ERS inhibitors taurine or 4-phenylbutyric acid attenuated ERS and VSMC apoptosis in calcified rat arteries, reduced calcification and retarded the VSMC contractile phenotype transforming into an osteoblast-like phenotype in vivo. Inhibition of ERS retarded the VSMC phenotypic transition into an osteoblast-like cell phenotype and reduced VSMC calcification and apoptosis in vitro. Interestingly, ATF4 was activated in calcified aortas and calcified VSMCs in vitro. ATF4 knockdown attenuated ERS-induced apoptosis in calcified VSMCs. ATF4 deficiency blocked VSMC calcification and negatively regulated the osteoblast phenotypic transition of VSMCs in vitro. Our results demonstrate that ATF4 was involved at least in part in the process of ERS-mediated apoptosis contributing to VC.

语种: 英语
所属项目编号: 81270407 ; 81170082 ; 20110001110012
项目资助者: National Natural Science Foundation of China ; Ministry of Education Foundation for Doctoral Tutor
WOS记录号: WOS:000322664700010
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56028
Appears in Collections:基础医学院_期刊论文

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作者单位: 1.Capital Med Univ, Key Lab Remodeling Related Cardiovasc Dis, Minist Educ, Beijing An Zhen Hosp, Beijing 100029, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Dept Physiol & Pathophysiol, Sch Basic Med Sci, Beijing 100191, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Key Lab Mol Cardiovasc Sci, Minist Educ, Beijing 100191, Peoples R China
4.Beijing Normal Univ, Sch PE & Sports Sci, Beijing 100875, Peoples R China
5.Hosp Tsinghua Univ, Beijing 100084, Peoples R China

Recommended Citation:
Duan, Xiao-Hui,Chang, Jin-Rui,Zhang, Jing,et al. Activating transcription factor 4 is involved in endoplasmic reticulum stress-mediated apoptosis contributing to vascular calcification[J]. APOPTOSIS,2013,18(9):1132-1144.
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