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学科主题: 药学
题名:
Simultaneous determination of nine major flavonoids in Sophora flavescens by RP-LC
作者: Ma, Xiao-Chi1,2; Xin, Xiu-Lan1,3; Liu, Ke-Xin2; Zhang, Bao-Jing2; Li, Feng-Yun2; Guo, De-An1
关键词: column liquid chromatography ; quality control ; flavonoids ; Sophora flavescens
刊名: CHROMATOGRAPHIA
发表日期: 2008-09-01
DOI: 10.1365/s10337-008-0719-y
卷: 68, 期:5-6, 页:471-474
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: PERFORMANCE LIQUID-CHROMATOGRAPHY ; CAPILLARY-ELECTROPHORESIS ; INHIBITORY FLAVONOIDS ; PRENYLATED FLAVONOIDS ; MEDICINAL-PLANTS ; CONSTITUENTS ; ROOTS ; AIT ; DAD ; ALKALOIDS
英文摘要:

A liquid chromatographic method was applied to determine trifolirhizin, kushenol K, kushenol L, kushenol N, kushenol X, kurarinone, norkurarinone, isokurarinone and kushenol A in the roots of Sophora flavescens, namely Kushen in China. The samples were separated on a YMC-C(18) column (250 x 4.6 mm, 5 mu m) with a gradient of methanol and 0.3% aqueous acetic acid (v/v) at a flow rate of 0.8 mL min(-1) and detected at 295 nm. The complete separation was achieved within 45 min for the nine major flavonoids. All calibration curves expressed good linearity (r(2) > 0.999) within the test range. The recovery of this method was 92.3-106.9%. The assay was successfully applied to the quantification of nine flavonoids in 26 samples of Kushen. The results indicated that this developed LC assay could be readily utilized as a quality control method for the Chinese herb medicine Kushen.

语种: 英语
所属项目编号: 985-2- 063-112 ; 30701088
项目资助者: Changjiang Scholars and Innovative Team of the University ; National Natural Science Foundation of China ; PHR (IHLB)
WOS记录号: WOS:000259186700027
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56035
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100083, Peoples R China
2.Dalian Med Univ, Sch Pharmaceut Sci, Dalian 116027, Peoples R China
3.Beijing Vocat Coll Elect Sci & Technol, Biotechnol Applicat Ctr, Beijing 100029, Peoples R China

Recommended Citation:
Ma, Xiao-Chi,Xin, Xiu-Lan,Liu, Ke-Xin,et al. Simultaneous determination of nine major flavonoids in Sophora flavescens by RP-LC[J]. CHROMATOGRAPHIA,2008,68(5-6):471-474.
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