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IR@PKUHSC  > 北京大学第一临床医学院  > 皮肤性病科  > 期刊论文
学科主题: 临床医学
题名:
Differentiation of clinically relevant Mucorales Rhizopus microsporus and R. arrhizus by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS)
作者: Dolatabadi, Somayeh1,2; Kolecka, Anna1; Versteeg, Matthijs1; de Hoog, Sybren G.1,2,3,4,5,6,7; Boekhout, Teun1,5
刊名: JOURNAL OF MEDICAL MICROBIOLOGY
发表日期: 2015-07-01
DOI: 10.1099/jmm.0.000091
卷: 64, 页:694-701
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Microbiology
研究领域[WOS]: Microbiology
关键词[WOS]: ANTIFUNGAL SUSCEPTIBILITY ; SPECIES IDENTIFICATION ; LABORATORY DIAGNOSIS ; FILAMENTOUS FUNGI ; MUCORMYCOSIS ; ZYGOMYCOSIS ; DISCRIMINATION ; PATHOGENESIS ; ASPERGILLUS ; PHYLOGENY
英文摘要:

This study addresses the usefulness of matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) MS for reliable identification of the two most frequently occurring clinical species of Rhizopus, namely Rhizopus arrhizus with its two varieties, arrhizus and delemar, and Rhizopus microsporus. The test-set comprised 38 isolates of clinical and environmental origin previously identified by internal transcribed spacer (ITS) sequencing of rDNA. Multi-locus sequence data targeting three gene markers (ITS, ACT, TEF) showed two monophylic clades for Rhizopus arrhizus and Rhizopus microsporus (bootstrap values of 99%). Cluster analysis confirmed the presence of two distinct clades within Rhizopus arrhizus representing its varieties arrhizus and delemar. The MALDI Biotyper 3.0 Microflex LT platform (Bruker Daltonics) was used to confirm the distinction between Rhizopus arrhizus and Rhizopus microsporus and the presence of two varieties within the species Rhizopus arrhizus. An in-house database of 30 reference main spectra (MSPs) was initially tested for correctness using commercially available databases of Bruker Daltonics. By challenging the database with the same strains of which an in-house database was created, automatic identification runs confirmed that MALDI-TOF MS is able to recognize the strains at the variety level. Based on principal component analysis, two MSP dendrograms were created and showed concordance with the multi-locus tree; thus, MALDI-TOF MS is a useful tool for diagnostics of mucoralean species.

语种: 英语
所属项目编号: 5-298-3-086
项目资助者: Qatar National Research Fund (a member of the Qatar Foundation) NPRP grant
WOS记录号: WOS:000360872300006
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56042
Appears in Collections:北京大学第一临床医学院_皮肤性病科_期刊论文

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作者单位: 1.CBS KNAW Fungal Biodivers Ctr, Utrecht, Netherlands
2.King Abdulaziz Univ, Jeddah 21413, Saudi Arabia
3.Univ Amsterdam, Inst Biodivers & Ecosyst Dynam, Amsterdam, Netherlands
4.Peking Univ, Hlth Sci Ctr, Res Ctr Med Mycol, Beijing 100871, Peoples R China
5.Sun Yat Sen Univ, Sun Yat Sen Hosp, Guangzhou 510275, Guangdong, Peoples R China
6.Second Mil Med Univ, Changzheng Hosp, Shanghai, Peoples R China
7.Univ Fed Parana, Basic Pathol Dept, Curitiba, Parana, Brazil

Recommended Citation:
Dolatabadi, Somayeh,Kolecka, Anna,Versteeg, Matthijs,et al. Differentiation of clinically relevant Mucorales Rhizopus microsporus and R. arrhizus by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS)[J]. JOURNAL OF MEDICAL MICROBIOLOGY,2015,64:694-701.
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