IR@PKUHSC  > 北京大学第一临床医学院  > 皮肤性病科
学科主题临床医学
Differentiation of clinically relevant Mucorales Rhizopus microsporus and R. arrhizus by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS)
Dolatabadi, Somayeh1,2; Kolecka, Anna1; Versteeg, Matthijs1; de Hoog, Sybren G.1,2,3,4,5,6,7; Boekhout, Teun1,5
刊名JOURNAL OF MEDICAL MICROBIOLOGY
2015-07-01
DOI10.1099/jmm.0.000091
64页:694-701
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Microbiology
资助者Qatar National Research Fund (a member of the Qatar Foundation) NPRP grant ; Qatar National Research Fund (a member of the Qatar Foundation) NPRP grant
研究领域[WOS]Microbiology
关键词[WOS]ANTIFUNGAL SUSCEPTIBILITY ; SPECIES IDENTIFICATION ; LABORATORY DIAGNOSIS ; FILAMENTOUS FUNGI ; MUCORMYCOSIS ; ZYGOMYCOSIS ; DISCRIMINATION ; PATHOGENESIS ; ASPERGILLUS ; PHYLOGENY
英文摘要

This study addresses the usefulness of matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) MS for reliable identification of the two most frequently occurring clinical species of Rhizopus, namely Rhizopus arrhizus with its two varieties, arrhizus and delemar, and Rhizopus microsporus. The test-set comprised 38 isolates of clinical and environmental origin previously identified by internal transcribed spacer (ITS) sequencing of rDNA. Multi-locus sequence data targeting three gene markers (ITS, ACT, TEF) showed two monophylic clades for Rhizopus arrhizus and Rhizopus microsporus (bootstrap values of 99%). Cluster analysis confirmed the presence of two distinct clades within Rhizopus arrhizus representing its varieties arrhizus and delemar. The MALDI Biotyper 3.0 Microflex LT platform (Bruker Daltonics) was used to confirm the distinction between Rhizopus arrhizus and Rhizopus microsporus and the presence of two varieties within the species Rhizopus arrhizus. An in-house database of 30 reference main spectra (MSPs) was initially tested for correctness using commercially available databases of Bruker Daltonics. By challenging the database with the same strains of which an in-house database was created, automatic identification runs confirmed that MALDI-TOF MS is able to recognize the strains at the variety level. Based on principal component analysis, two MSP dendrograms were created and showed concordance with the multi-locus tree; thus, MALDI-TOF MS is a useful tool for diagnostics of mucoralean species.

语种英语
所属项目编号5-298-3-086
资助者Qatar National Research Fund (a member of the Qatar Foundation) NPRP grant ; Qatar National Research Fund (a member of the Qatar Foundation) NPRP grant
WOS记录号WOS:000360872300006
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56042
Collection北京大学第一临床医学院_皮肤性病科
作者单位1.CBS KNAW Fungal Biodivers Ctr, Utrecht, Netherlands
2.King Abdulaziz Univ, Jeddah 21413, Saudi Arabia
3.Univ Amsterdam, Inst Biodivers & Ecosyst Dynam, Amsterdam, Netherlands
4.Peking Univ, Hlth Sci Ctr, Res Ctr Med Mycol, Beijing 100871, Peoples R China
5.Sun Yat Sen Univ, Sun Yat Sen Hosp, Guangzhou 510275, Guangdong, Peoples R China
6.Second Mil Med Univ, Changzheng Hosp, Shanghai, Peoples R China
7.Univ Fed Parana, Basic Pathol Dept, Curitiba, Parana, Brazil
Recommended Citation
GB/T 7714
Dolatabadi, Somayeh,Kolecka, Anna,Versteeg, Matthijs,et al. Differentiation of clinically relevant Mucorales Rhizopus microsporus and R. arrhizus by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS)[J]. JOURNAL OF MEDICAL MICROBIOLOGY,2015,64:694-701.
APA Dolatabadi, Somayeh,Kolecka, Anna,Versteeg, Matthijs,de Hoog, Sybren G.,&Boekhout, Teun.(2015).Differentiation of clinically relevant Mucorales Rhizopus microsporus and R. arrhizus by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS).JOURNAL OF MEDICAL MICROBIOLOGY,64,694-701.
MLA Dolatabadi, Somayeh,et al."Differentiation of clinically relevant Mucorales Rhizopus microsporus and R. arrhizus by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS)".JOURNAL OF MEDICAL MICROBIOLOGY 64(2015):694-701.
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