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学科主题基础医学
The effects of amyloid-beta(42) oligomer on the proliferation and activation of astrocytes in vitro
Hou, Lingling1; Liu, Yanfeng1; Wang, Xiaoyu1; Ma, Haibin1; He, Jinsheng1; Zhang, Ying1; Yu, Changhai2; Guan, Weijun3; Ma, Yuehui3
关键词Alzheimer&prime s disease Astrocyte beta-amyloid (A beta(42)) peptide Proliferation Activation
刊名IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
2011-09-01
DOI10.1007/s11626-011-9439-y
47期:8页:573-580
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology ; Developmental Biology
研究领域[WOS]Cell Biology ; Developmental Biology
关键词[WOS]ALZHEIMERS-DISEASE ; NEUROTROPHIC FACTOR ; AMYLOID PLAQUES ; BRAIN-REGIONS ; INFLAMMATION ; PEPTIDES ; REVERSAL ; CELLS
英文摘要

Previous studies reported that astrocyte response to amyloid-beta (A beta) before obvious neuronal damage could be detected in Alzheimer′s disease (AD). It is suggested that astrocytes play a key role in AD pathologies. In this study, we investigated the effects of A beta(42) oligomer on the proliferation and activation of astrocytes by in vitro experiments. The results showed that A beta(42) oligomers could convert astrocytes to responsive astrocytes. It was revealed by MTT and ELISA assays that the viability of astrocytes gradually decreased, and the release of brain-derived neurotrophic factor increased with elevated A beta(42) concentration and prolonged duration. Reverse-transcription polymerase chain reaction (RT-PCR) assay indicated that A beta(42) oligomers increased the expression of glial fibers acid protein and interleukin-1 beta in a dose-dependent but not time-dependent manner. It was showed that A8, a mouse monoclonal antibody, was able to protect the cultured astrocytes against the toxicity of A beta(42) oligomers. The result demonstrated that A8 could inhibit A beta(42) oligomers toxic effects on astrocytes and that, alone, A8 could promote the proliferation of astrocytes in certain time. The present study laid a theoretical foundation for further understanding the effects of A beta(42) on astrocytes and, hence, is conducive to the theoretical understanding and clinical therapies of AD progression.

语种英语
WOS记录号WOS:000298229800011
项目编号2005DKA21101 ; 2008BAK41B01-5 ; 2008ZX08009-003
资助机构National Infrastructure of Natural Science and Technology Program ; Key Projects in the National Science and Technology Pillar Program ; Ministry of Agriculture of China
引用统计
被引频次:20[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56043
专题北京大学基础医学院_神经生物学系
北京大学基础医学院
作者单位1.Beijing Jiaotong Univ, Coll Life Sci & Bioengn, Beijing 100044, Peoples R China
2.Peking Univ, Dept Neurobiol, Sch Basic Med, Hlth Sci Ctr, Beijing 100191, Peoples R China
3.Chinese Acad Agr Sci, Inst Anim Sci, Beijing 100193, Peoples R China
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GB/T 7714
Hou, Lingling,Liu, Yanfeng,Wang, Xiaoyu,et al. The effects of amyloid-beta(42) oligomer on the proliferation and activation of astrocytes in vitro[J]. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL,2011,47(8):573-580.
APA Hou, Lingling.,Liu, Yanfeng.,Wang, Xiaoyu.,Ma, Haibin.,He, Jinsheng.,...&Ma, Yuehui.(2011).The effects of amyloid-beta(42) oligomer on the proliferation and activation of astrocytes in vitro.IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL,47(8),573-580.
MLA Hou, Lingling,et al."The effects of amyloid-beta(42) oligomer on the proliferation and activation of astrocytes in vitro".IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL 47.8(2011):573-580.
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