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学科主题: 临床医学
题名:
Tumor suppressive microRNA-200a inhibits renal cell carcinoma development by directly targeting TGFB2
作者: Lu, Ruijing1,2; Ji, Ziliang3; Li, Xiaoqing1; Qin, Jie1; Cui, Guanghui1; Chen, Jing1; Zhai, Qingna1,2; Zhao, Chunjuan1; Zhang, Wei4; Yu, Zhendong1
关键词: miR-200a ; Tumor suppressor ; TGFB2 ; Renal cell carcinoma
刊名: TUMOR BIOLOGY
发表日期: 2015-09-01
DOI: 10.1007/s13277-015-3355-9
卷: 36, 期:9, 页:6691-6700
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: EPITHELIAL-MESENCHYMAL TRANSITION ; MIR-200 FAMILY ; MOLECULAR TARGETS ; REPRESSORS ZEB1 ; CANCER-CELLS ; EXPRESSION ; MIGRATION ; GROWTH ; PROGRESSION ; METASTASIS
英文摘要:

A large body of evidence indicates that microRNAs play a critical role in tumor initiation and progression by negatively regulating oncogenes or tumor suppressor genes. Here, we report that the expression of miR-200a was notably downregulated in 45 renal cell carcinoma (RCC) samples. Restoration of miR-200a suppressed cell proliferation, migration, and invasion in two RCC cell lines. Furthermore, we used an epithelial-to-mesenchymal transition PCR array to explore the putative target genes of miR-200a. By performing quantitative real-time PCR, ELISA, and luciferase reporter assays, transforming growth factor beta2 (TGFB2) was validated as a direct target gene of miR-200a. Moreover, siRNA-mediated knockdown of TGFB2 partially phenocopied the effect of miR-200a overexpression. These results suggest that miR-200a suppresses RCC development via directly targeting TGFB2, indicating that miR-200a may present a novel target for diagnostic and therapeutic strategies in RCC.

语种: 英语
所属项目编号: JCYJ20140415162543037 ; 201401049
项目资助者: Science and Technology Planning Project of Shenzhen in China ; Health and Family Planning Scientific Research Project of Shenzhen in China
WOS记录号: WOS:000365033100014
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56055
Appears in Collections:北京大学深圳医院_中心实验室_期刊论文

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作者单位: 1.Peking Univ, Shenzhen Hosp, Cent Lab, Shenzhen 518036, Peoples R China
2.Baoan Dist Shenzhen Matern & Child Hlth Hosp, Clin Lab, Shenzhen 518133, Peoples R China
3.Longgang Dist Peoples Hosp Shenzhen, Dept Urol, Shenzhen 518172, Peoples R China
4.Hong Kong Univ Sci & Technol, Shenzhen Peking Univ, Biomed Res Inst, Shenzhen 518036, Peoples R China

Recommended Citation:
Lu, Ruijing,Ji, Ziliang,Li, Xiaoqing,et al. Tumor suppressive microRNA-200a inhibits renal cell carcinoma development by directly targeting TGFB2[J]. TUMOR BIOLOGY,2015,36(9):6691-6700.
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