IR@PKUHSC  > 北京大学第一临床医学院  > 肾脏内科
学科主题临床医学
Therapeutic approach for diabetic nephropathy using gene delivery of translocase of inner mitochondrial membrane 44 by reducing mitochondrial superoxide production
Zhang, Yanling; Wada, Jun; Hashimoto, Izumi; Eguchi, Jun; Yasuhara, Akihiro; Kanwar, Yashpal S.; Shikata, Kenichi; Makino, Hirofumi
刊名JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
2006-04-01
DOI10.1681/ASN.2005111148
17期:4页:1090-1101
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Urology & Nephrology
研究领域[WOS]Urology & Nephrology
关键词[WOS]PROXIMAL TUBULE CELLS ; PROTEIN IMPORT ; OXIDATIVE STRESS ; RENAL-FAILURE ; MEMBRANE ; PREPROTEINS ; CULTURE ; RATS ; COMPLICATIONS ; RECEPTORS
英文摘要

Hyperglycemia-induced overproduction of mitochondrial reactive oxygen species has emerged as a major player in diabetic vascular complications. Mammalian translocase of inner mitochondrial membrane 44 (TIM44) was identified by upregulation in diabetic mouse kidneys. TIM44 functions as a membrane anchor of mitochondrial heat-shock protein 70 (mtHsp70) to TIM23 complex and is involved in the import of mitochondria-targeted preproteins into mitochondrial matrix. The process is dependent on inner membrane potential and ATP hydrolysis on ATPase domain of mitochondrial heat-shock protein 70. Hemagglutination virus of Japan-envelope vector that carries pcDNA3.1 plasmid that contains the full-length cDNA of TIM44 and control plasmid were injected weekly into the tail vein of uninephrectomized streptozotocin-induced diabetic CD-1 mice. The gene delivery alleviated proteinuria and renal hypertrophy at 8 wk after the injection, inhibited renal cell proliferation and apoptosis, and suppressed superoxide production. In vitro experiments, using human proximal tubular (HK2) cells, revealed that the gene delivery of TIM44 reversed high glucose-induced metabolic and cellular abnormalities such as enhanced reactive oxygen species production, increased ATP contents, alterations in inner membrane potential, increased cell proliferation, and apoptosis. Transfection with siRNA and expressing vector of TIM44 revealed that TIM44 facilitates import of antioxidative enzymes such as superoxide dismutase and glutathione peroxidase into mitochondria. The gene delivery of TIM44 therefore seems to be beneficial for the maintenance of mitochondrial function and is a novel therapeutic approach for diabetic nephropathy.

语种英语
WOS记录号WOS:000242120600023
引用统计
被引频次:49[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56077
专题北京大学第一临床医学院_肾脏内科
作者单位1.Okayama Univ, Grad Sch Med, Dept Med & Clin Sci, Okayama 7008558, Japan
2.Peking Univ, Hosp 1, Div Renal, Inst Nephrol, Beijing 100871, Peoples R China
3.Northwestern Univ, Feinberg Sch Med, Dept Pathol, Chicago, IL 60611 USA
推荐引用方式
GB/T 7714
Zhang, Yanling,Wada, Jun,Hashimoto, Izumi,et al. Therapeutic approach for diabetic nephropathy using gene delivery of translocase of inner mitochondrial membrane 44 by reducing mitochondrial superoxide production[J]. JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY,2006,17(4):1090-1101.
APA Zhang, Yanling.,Wada, Jun.,Hashimoto, Izumi.,Eguchi, Jun.,Yasuhara, Akihiro.,...&Makino, Hirofumi.(2006).Therapeutic approach for diabetic nephropathy using gene delivery of translocase of inner mitochondrial membrane 44 by reducing mitochondrial superoxide production.JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY,17(4),1090-1101.
MLA Zhang, Yanling,et al."Therapeutic approach for diabetic nephropathy using gene delivery of translocase of inner mitochondrial membrane 44 by reducing mitochondrial superoxide production".JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY 17.4(2006):1090-1101.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Zhang, Yanling]的文章
[Wada, Jun]的文章
[Hashimoto, Izumi]的文章
百度学术
百度学术中相似的文章
[Zhang, Yanling]的文章
[Wada, Jun]的文章
[Hashimoto, Izumi]的文章
必应学术
必应学术中相似的文章
[Zhang, Yanling]的文章
[Wada, Jun]的文章
[Hashimoto, Izumi]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。