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Expression Profile of a Novel Germ Cell-specific Gene, TSCPA, in Mice and Human
Yu, Zhendong1; Wu, Bo1; Tang, Aifa2; Chen, Jing2; Guo, Xin2; Qin, Jie2; Gui, Yaoting2; Cai, Zhiming2
关键词Dna Microarray Tscpa Gene Spermatogenesis Sertoli-cell-only Syndrome Cryptorchidism
刊名JOURNAL OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY-MEDICAL SCIENCES
2009-10-01
DOI10.1007/s11596-009-0502-9
29期:5页:535-539
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]MOUSE SPERMATOGENESIS ; HUMAN TESTIS ; IDENTIFICATION ; PROTEIN ; RNA
英文摘要

In order to identify novel genes involved in spermatogenesis, testis cDNA samples from Balb/C mice of different postnatal days were hybridized with the whole mouse genome Affymetrix chip to screen the testis-specific genes. The characteristics of the selected genes were analyzed by RT-PCR as well as other bioinformatic tools. A novel differentially expressed testis-specific gene (GenBank Accession No: NM_029042) in the developmental stages of testes was identified, and named TSCPA. Cellular mapping prediction of TSCPA indicated that its protein was probably expressed in nuclei, and one putative domain (aa 332-377) was anchoring domain of cAMP-dependent type. PK. The result of subcellular localization of GFP-TSCPA fusion protein in Cos-7 cells showed that TSCPA protein was expressed in nuclei. RT-PCR analysis revealed that TSCPA was expressed specifically in mouse and human testis. TSCPA gene was expressed weakly in 21-day-old mouse testis and the expression was increased gradually from 38th day to 6th month of mouse testes. No expression of hTSCPA was found in cryptorchidism and Sertoli-cell-only syndrome patients. It was concluded that the expression profile of TSCPA in human and mice indicated that TSCPA might play an important role in spermatogenesis.

语种英语
WOS记录号WOS:000270681700002
引用统计
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56097
专题北京大学深圳医院
作者单位1.Peking Univ, Shenzhen Hosp, Dept Clin Lab, Shenzhen 518036, Peoples R China
2.Peking Univ, Shenzhen Hosp, Guangdong & Shenzhen Key Lab Male Reprod Med & Ge, Shenzhen 518036, Peoples R China
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Yu, Zhendong,Wu, Bo,Tang, Aifa,et al. Expression Profile of a Novel Germ Cell-specific Gene, TSCPA, in Mice and Human[J]. JOURNAL OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY-MEDICAL SCIENCES,2009,29(5):535-539.
APA Yu, Zhendong.,Wu, Bo.,Tang, Aifa.,Chen, Jing.,Guo, Xin.,...&Cai, Zhiming.(2009).Expression Profile of a Novel Germ Cell-specific Gene, TSCPA, in Mice and Human.JOURNAL OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY-MEDICAL SCIENCES,29(5),535-539.
MLA Yu, Zhendong,et al."Expression Profile of a Novel Germ Cell-specific Gene, TSCPA, in Mice and Human".JOURNAL OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY-MEDICAL SCIENCES 29.5(2009):535-539.
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