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学科主题: 临床医学
题名:
Expression Profile of a Novel Germ Cell-specific Gene, TSCPA, in Mice and Human
作者: Yu, Zhendong1; Wu, Bo1; Tang, Aifa2; Chen, Jing2; Guo, Xin2; Qin, Jie2; Gui, Yaoting2; Cai, Zhiming2
关键词: DNA microarray ; TSCPA gene ; spermatogenesis ; Sertoli-cell-only syndrome ; cryptorchidism
刊名: JOURNAL OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY-MEDICAL SCIENCES
发表日期: 2009-10-01
DOI: 10.1007/s11596-009-0502-9
卷: 29, 期:5, 页:535-539
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
关键词[WOS]: MOUSE SPERMATOGENESIS ; HUMAN TESTIS ; IDENTIFICATION ; PROTEIN ; RNA
英文摘要:

In order to identify novel genes involved in spermatogenesis, testis cDNA samples from Balb/C mice of different postnatal days were hybridized with the whole mouse genome Affymetrix chip to screen the testis-specific genes. The characteristics of the selected genes were analyzed by RT-PCR as well as other bioinformatic tools. A novel differentially expressed testis-specific gene (GenBank Accession No: NM_029042) in the developmental stages of testes was identified, and named TSCPA. Cellular mapping prediction of TSCPA indicated that its protein was probably expressed in nuclei, and one putative domain (aa 332-377) was anchoring domain of cAMP-dependent type. PK. The result of subcellular localization of GFP-TSCPA fusion protein in Cos-7 cells showed that TSCPA protein was expressed in nuclei. RT-PCR analysis revealed that TSCPA was expressed specifically in mouse and human testis. TSCPA gene was expressed weakly in 21-day-old mouse testis and the expression was increased gradually from 38th day to 6th month of mouse testes. No expression of hTSCPA was found in cryptorchidism and Sertoli-cell-only syndrome patients. It was concluded that the expression profile of TSCPA in human and mice indicated that TSCPA might play an important role in spermatogenesis.

语种: 英语
所属项目编号: 7008952 ; 7300931 ; 30700824
项目资助者: Natural Science Foundation of Guangdong Province, China ; National Natural Sciences Foundation of China
WOS记录号: WOS:000270681700002
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56097
Appears in Collections:北京大学深圳医院_期刊论文

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作者单位: 1.Peking Univ, Shenzhen Hosp, Dept Clin Lab, Shenzhen 518036, Peoples R China
2.Peking Univ, Shenzhen Hosp, Guangdong & Shenzhen Key Lab Male Reprod Med & Ge, Shenzhen 518036, Peoples R China

Recommended Citation:
Yu, Zhendong,Wu, Bo,Tang, Aifa,et al. Expression Profile of a Novel Germ Cell-specific Gene, TSCPA, in Mice and Human[J]. JOURNAL OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY-MEDICAL SCIENCES,2009,29(5):535-539.
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