北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学口腔医学院  > 口腔种植科  > 期刊论文
学科主题: 口腔医学
题名:
P-gingivalis Modulates Keratinocytes through FOXO Transcription Factors
作者: Li, Shuai1,2,3; Dong, Guangyu2,3; Moschidis, Anastasios4; Ortiz, Javier4; Benakanakere, Manjunatha R.2,3; Kinane, Denis F.2,3; Graves, Dana T.2,3
刊名: PLOS ONE
发表日期: 2013-11-12
DOI: 10.1371/journal.pone.0078541
卷: 8, 期:11
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: EPITHELIAL-CELLS ; IN-VITRO ; FIBROBLAST APOPTOSIS ; EXPRESSION ; GINGIPAIN ; BACTERIA ; DEGRADATION ; MECHANISM ; INFECTION ; PROTEINS
英文摘要:

P. gingivalis is a prominent periodontal pathogen that has potent effects on host cells. In this study we challenged gingival epithelial cells with P. gingivalis with the aim of assessing how mRNA levels of key target genes were modulated by P. gingivalis via the transcription factors FOXO1 and FOXO3. Primary mono- and multi-layer cultures of gingival epithelial cells were challenged and barrier function was examined by fluorescent dextran and apoptosis was measured by cytoplasmic histone associated DNA. Gene expression levels were measured by real-time PCR with and without FOXO1 and FOXO3 siRNA compared to scrambled siRNA. P. gingivalis induced a loss of barrier function and stimulated gingival epithelial cell apoptosis in multilayer cultures that was in part gingipain dependent. P. gingivalis stimulated an increase in FOXO1 and FOXO3 mRNA, enhanced mRNA levels of genes associated with differentiated keratinocyte function (keratin-1, -10, -14, and involucrin), increased mRNA levels of apoptotic genes (BID and TRADD), reduced mRNA levels of genes that regulate inflammation (TLR-2 and -4) and reduced those associated with barrier function (integrin beta-1, -3 and -6). The ability of P. gingivalis to modulate these genes was predominantly FOXO1 and FOXO3 dependent. The results indicate that P. gingivalis has pronounced effects on gingival keratinocytes and modulates mRNA levels of genes that affect host response, differentiation, apoptosis and barrier function. Moreover, this modulation is dependent upon the transcription factors FOXO1 or FOXO3. In addition, a new function for FOXO1 was identified, that of suppressing TLR-2 and TLR-4 and maintaining integrin beta -1, beta -3 and beta -6 basal mRNA levels in keratinocytes.

语种: 英语
所属项目编号: DE018307 ; DE019108
项目资助者: NIDCR
WOS记录号: WOS:000327252100029
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56104
Appears in Collections:北京大学口腔医学院_口腔种植科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Peking Univ, Dept Implant Dent, Sch & Hosp Stomatol, Beijing 100871, Peoples R China
2.Univ Penn, Sch Dent Med, Dept Pathol, Philadelphia, PA 19104 USA
3.Univ Penn, Sch Dent Med, Dept Periodont, Philadelphia, PA 19104 USA
4.Boston Univ, Sch Dent Med, Dept Periodontol & Oral Biol, Boston, MA 02215 USA

Recommended Citation:
Li, Shuai,Dong, Guangyu,Moschidis, Anastasios,et al. P-gingivalis Modulates Keratinocytes through FOXO Transcription Factors[J]. PLOS ONE,2013,8(11).
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Li, Shuai]'s Articles
[Dong, Guangyu]'s Articles
[Moschidis, Anastasios]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Li, Shuai]‘s Articles
[Dong, Guangyu]‘s Articles
[Moschidis, Anastasios]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace