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学科主题: 药学
题名:
Synergistic Effect of Ethaselen and Selenite Treatment against A549 Human Non-small Cell Lung Cancer Cells
作者: Xu, Wei1,2; Ma, Wei-Wei1,2; Zeng, Hui-Hui1,2
关键词: Ethaselen ; selenite ; synergism ; oxidative stress ; A549 NSCLC cells
刊名: ASIAN PACIFIC JOURNAL OF CANCER PREVENTION
发表日期: 2014
DOI: 10.7314/APJCP.2014.15.17.7129
卷: 15, 期:17, 页:7129-7135
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: THIOREDOXIN REDUCTASE ; EXPRESSION ; APOPTOSIS ; LINES ; SUPPLEMENTATION ; PROLIFERATION ; COMBINATION
英文摘要:

Background: In this study, we aimed to evaluate the growth inhibitory effect of the combination of ethaselen (BBSKE) and low fixed dose of selenite against A549 human non-small cell lung cancer cells in vitro. Materials and Methods: Growth inhibitory effects against A549 cells were determined by SRB assay. Combination index (CI) values were calculated based on Chou-Talalay median-effect analyses. Dose reduction index (DRI) values were applied to calculate dose reduction of selenite. Contents of free thiols and GSH were determined by DTNB assay and intracellular ROS levels by DCFH-DA fluorescence labeling. Results: Compared with BBSKE or selenite single treatment, the combined application of ethaselen and a low fixed dose of selenite shortened the onset time of sodium selenite, reduced IC50 values, and increased the maximum inhibition rates, suggesting a possible molecular mechanism of the synergism. Obvious synergistic effects were observed after different times of combination treatment, especially after 24 h. Compared with selenite single treatment, dosage of selenite could be remarkably reduced in combination therapy to gain the same inhibitory effect on cell proliferation. Compared with BBSKE single treatment, the content of free thiols and GSH were significantly reduced and ROS levels greatly elevated in the combination group. For the combination treatment, cell viability increased as greater concentrations of GSH were added. Conclusions: All these results indicate that the combination treatment of BBSKE and selenite showed synergism to inhibit A549 cell proliferation in vitro, and also reduced the selenite dosage to mitigate its toxicity which is very meaningful for combination chemotherapy of lung cancer. The synergism was probably caused by the accelerated exhaustion of intracellular reductive substances, such as free thiols and GSH, which ultimately leads to enhanced oxidative stress and apoptosis.

语种: 英语
所属项目编号: 81372266 ; 2011zx09101-001-03
项目资助者: National Natural Science Foundation ; National Science and Technology Major Project, People&prime ; s Republic of China
WOS记录号: WOS:000343833100022
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56110
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Peking Univ, State Key Lab Nat & Biomimet Drugs, Beijing 100871, Peoples R China
2.Peking Univ, Sch Pharmaceut Sci, Beijing 100871, Peoples R China

Recommended Citation:
Xu, Wei,Ma, Wei-Wei,Zeng, Hui-Hui. Synergistic Effect of Ethaselen and Selenite Treatment against A549 Human Non-small Cell Lung Cancer Cells[J]. ASIAN PACIFIC JOURNAL OF CANCER PREVENTION,2014,15(17):7129-7135.
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