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Synergistic Effect of Ethaselen and Selenite Treatment against A549 Human Non-small Cell Lung Cancer Cells
Xu, Wei1,2; Ma, Wei-Wei1,2; Zeng, Hui-Hui1,2
关键词Ethaselen Selenite Synergism Oxidative Stress A549 Nsclc Cells
刊名ASIAN PACIFIC JOURNAL OF CANCER PREVENTION
2014
DOI10.7314/APJCP.2014.15.17.7129
15期:17页:7129-7135
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
研究领域[WOS]Oncology
关键词[WOS]THIOREDOXIN REDUCTASE ; EXPRESSION ; APOPTOSIS ; LINES ; SUPPLEMENTATION ; PROLIFERATION ; COMBINATION
英文摘要

Background: In this study, we aimed to evaluate the growth inhibitory effect of the combination of ethaselen (BBSKE) and low fixed dose of selenite against A549 human non-small cell lung cancer cells in vitro. Materials and Methods: Growth inhibitory effects against A549 cells were determined by SRB assay. Combination index (CI) values were calculated based on Chou-Talalay median-effect analyses. Dose reduction index (DRI) values were applied to calculate dose reduction of selenite. Contents of free thiols and GSH were determined by DTNB assay and intracellular ROS levels by DCFH-DA fluorescence labeling. Results: Compared with BBSKE or selenite single treatment, the combined application of ethaselen and a low fixed dose of selenite shortened the onset time of sodium selenite, reduced IC50 values, and increased the maximum inhibition rates, suggesting a possible molecular mechanism of the synergism. Obvious synergistic effects were observed after different times of combination treatment, especially after 24 h. Compared with selenite single treatment, dosage of selenite could be remarkably reduced in combination therapy to gain the same inhibitory effect on cell proliferation. Compared with BBSKE single treatment, the content of free thiols and GSH were significantly reduced and ROS levels greatly elevated in the combination group. For the combination treatment, cell viability increased as greater concentrations of GSH were added. Conclusions: All these results indicate that the combination treatment of BBSKE and selenite showed synergism to inhibit A549 cell proliferation in vitro, and also reduced the selenite dosage to mitigate its toxicity which is very meaningful for combination chemotherapy of lung cancer. The synergism was probably caused by the accelerated exhaustion of intracellular reductive substances, such as free thiols and GSH, which ultimately leads to enhanced oxidative stress and apoptosis.

语种英语
WOS记录号WOS:000343833100022
项目编号81372266 ; 2011zx09101-001-03
资助机构National Natural Science Foundation ; National Science and Technology Major Project, People&prime ; s Republic of China
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56110
专题北京大学药学院
作者单位1.Peking Univ, State Key Lab Nat & Biomimet Drugs, Beijing 100871, Peoples R China
2.Peking Univ, Sch Pharmaceut Sci, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Xu, Wei,Ma, Wei-Wei,Zeng, Hui-Hui. Synergistic Effect of Ethaselen and Selenite Treatment against A549 Human Non-small Cell Lung Cancer Cells[J]. ASIAN PACIFIC JOURNAL OF CANCER PREVENTION,2014,15(17):7129-7135.
APA Xu, Wei,Ma, Wei-Wei,&Zeng, Hui-Hui.(2014).Synergistic Effect of Ethaselen and Selenite Treatment against A549 Human Non-small Cell Lung Cancer Cells.ASIAN PACIFIC JOURNAL OF CANCER PREVENTION,15(17),7129-7135.
MLA Xu, Wei,et al."Synergistic Effect of Ethaselen and Selenite Treatment against A549 Human Non-small Cell Lung Cancer Cells".ASIAN PACIFIC JOURNAL OF CANCER PREVENTION 15.17(2014):7129-7135.
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