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Induction of hepatoma carcinoma cell apoptosis through activation of the JNK-nicotinamide adenine dinucleotide phosphate (NADPH) oxidase-ROS self-driven death signal circuit
Zeng, Ke-Wu1; Song, Fang-Jiao2; Wang, Ying-Hong2; Li, Ning1; Yu, Qian2; Liao, Li-Xi1; Jiang, Yong1; Tu, Peng-Fei1
关键词Reactive Oxygen Species Nadph Oxidase Jnk Mapk Self-driven Signal Circuit
刊名CANCER LETTERS
2014-10-28
DOI10.1016/j.canlet.2014.07.022
353期:2页:220-231
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
研究领域[WOS]Oncology
关键词[WOS]SMALL-MOLECULE ANTAGONISTS ; OXYGEN SPECIES ROS ; COMPONENT P47(PHOX) ; DNA-DAMAGE ; NOX FAMILY ; PHOSPHORYLATION ; MITOCHONDRIA ; ASSAY ; ANGIOGENESIS ; P22(PHOX)
英文摘要

As an efficient method for inducing tumor cell apoptosis, ROS can be constantly formed and accumulated in NADPH oxidase overactivated-cells, resulting in further mitochondrial membrane damage and mitochondria-dependent apoptosis. In addition, JNK mitogen-activated protein kinase (JNK MAPK) signal also acts as a vital candidate pathway for inducing tumor cell apoptosis by targeting mitochondrial death pathway. However, the relationship between NADPH oxidase-ROS and JNK MAPK signal still remains unclear. Here, we discovered a novel self-driven signal circuit between NADPH oxidase-ROS and JNK MAPK, which was induced by a cytotoxic steroidal saponin (ASC) in hepatoma carcinoma cells. NADPH oxidase-dependent ROS production was markedly activated by ASC and directly led to JNK MAPK activation. Moreover, antioxidant, NADPH oxidase inhibitor and specific knock-out for p47 subunit of NADPH oxidase could effectively block NADPH oxidase-ROS-dependent JNK activation, suggesting that NADPH oxidase is an upstream regulator of JNK MAPK. Conversely, a specific JNK inhibitor could inhibit ASC-induced NADPH oxidase activation and down-regulate ROS levels as well, indicating that JNK might also regulate NADPH oxidase activity to some extent. These observations indicate that NADPH oxidase and JNK MAPK activate each other as a signal circuit. Furthermore, drug pretreatment experiments with ASC showed this signal circuit operated continuously via a self-driven mode and finally induced apoptosis in hepatoma carcinoma cells. Taken together, we provide a proof for inducing hepatoma carcinoma cell apoptosis by activating the JNK-NADPH oxidase-ROS-dependent self-driven signal circuit pathway. (C) 2014 Elsevier Ireland Ltd. All rights reserved.

语种英语
WOS记录号WOS:000342874800011
项目编号2012ZX09301002-002-002 ; 81303253 ; 30873072
资助机构National Key Technology R &amp ; D Program "New Drug Innovation" of China ; Natural Science Foundation of China
引用统计
被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56122
专题北京大学药学院
北京大学药学院_天然药物学系
作者单位1.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
2.Peking Univ, Hosp 1, Res Studio Integrat Tradit & Western Med, Beijing 100034, Peoples R China
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GB/T 7714
Zeng, Ke-Wu,Song, Fang-Jiao,Wang, Ying-Hong,et al. Induction of hepatoma carcinoma cell apoptosis through activation of the JNK-nicotinamide adenine dinucleotide phosphate (NADPH) oxidase-ROS self-driven death signal circuit[J]. CANCER LETTERS,2014,353(2):220-231.
APA Zeng, Ke-Wu.,Song, Fang-Jiao.,Wang, Ying-Hong.,Li, Ning.,Yu, Qian.,...&Tu, Peng-Fei.(2014).Induction of hepatoma carcinoma cell apoptosis through activation of the JNK-nicotinamide adenine dinucleotide phosphate (NADPH) oxidase-ROS self-driven death signal circuit.CANCER LETTERS,353(2),220-231.
MLA Zeng, Ke-Wu,et al."Induction of hepatoma carcinoma cell apoptosis through activation of the JNK-nicotinamide adenine dinucleotide phosphate (NADPH) oxidase-ROS self-driven death signal circuit".CANCER LETTERS 353.2(2014):220-231.
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