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Crystal structure of RS21-C6, involved in nucleoside triphosphate pyrophosphohydrolysis
Wu, Beili; Liu, Yuanfeng; Zhao, Qiang; Liao, Shuang; Zhang, Jun; Bartlam, Mark; Chen, Weifeng; Rao, Zihe
关键词RS21-C6 dUTPase pyrophosphohydrolase crystal structure kinetic activity
刊名JOURNAL OF MOLECULAR BIOLOGY
2007-04-13
DOI10.1016/j.jmb.2007.01.057
367期:5页:1405-1412
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]DUTPASES
英文摘要

RS21-C6, which is highly expressed in all vertebrate genomes and green plants, is proposed to have nucleoside triphosphate pyrophosphohydrolase activity. Here, we report the crystal structures of the core fragment of RS21-C6, named RSCUT, and the complex with the substrate 5-methyl dCTP. The refined structure of RSCUT consists mainly of alpha-helices and shows formation of a tightly associated tetramer. On the basis of the structure of the RSCUT-m5dCTP complex and the results of pyrophosphatase activity assays, several key residues involved in the substrate binding of RS21-C6 have been identified. Tetramer formation is shown to be required for substrate binding. (c) 2007 Elsevier Ltd. All rights reserved.

语种英语
WOS记录号WOS:000245508200015
引用统计
被引频次:13[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56123
专题基础医学院_免疫学系
作者单位1.Peking Univ, Hlth Sci Ctr, Dept Immunol, Sch Basic Med Sci, Beijing 100083, Peoples R China
2.Nankai Univ, Tianjin 300071, Peoples R China
3.Tsinghua Univ, Tsinghua IBP Nankai Joint Res Grp Struct Biol, Beijing 100084, Peoples R China
4.Chinese Acad Sci, Natl Lab Biomacromol, Inst Biophys, Beijing 100101, Peoples R China
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GB/T 7714
Wu, Beili,Liu, Yuanfeng,Zhao, Qiang,et al. Crystal structure of RS21-C6, involved in nucleoside triphosphate pyrophosphohydrolysis[J]. JOURNAL OF MOLECULAR BIOLOGY,2007,367(5):1405-1412.
APA Wu, Beili.,Liu, Yuanfeng.,Zhao, Qiang.,Liao, Shuang.,Zhang, Jun.,...&Rao, Zihe.(2007).Crystal structure of RS21-C6, involved in nucleoside triphosphate pyrophosphohydrolysis.JOURNAL OF MOLECULAR BIOLOGY,367(5),1405-1412.
MLA Wu, Beili,et al."Crystal structure of RS21-C6, involved in nucleoside triphosphate pyrophosphohydrolysis".JOURNAL OF MOLECULAR BIOLOGY 367.5(2007):1405-1412.
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