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学科主题: 临床医学
题名:
Development of ELISA for the specific determination of autoantibodies against envoplakin and periplakin in paraneoplastic pemphigus
作者: Probst, Christian1; Schlumberger, Wolfgang1; Stoecker, Winfried1; Recke, Andreas2; Schmidt, Enno2; Hashimoto, Takashi3; Zhu, Xue Jun4; Zillikens, Detlef2; Komorowski, Lars1
关键词: Desmosome ; Diagnosis ; Autoantibody ; Immunofluorescence microscopy
刊名: CLINICA CHIMICA ACTA
发表日期: 2009-12-08
DOI: 10.1016/j.cca.2009.08.022
卷: 410, 期:1-2, 页:13-18
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medical Laboratory Technology
研究领域[WOS]: Medical Laboratory Technology
关键词[WOS]: CORNIFIED ENVELOPE ; ANTI-ENVOPLAKIN ; PLAKIN FAMILY ; DESMOPLAKIN ; ANTIBODIES ; VULGARIS ; EPITOPES ; DOMAIN ; ASSAY ; SERA
英文摘要:

Introduction: In paraneoplastic pemphigus (PNP), indirect immunofluorescence microscopy using rat bladder sections is widely used to search for circulating autoantibodies which are predominantly directed against envoplakin and periplakin. A sensitive and specific detection system for envoplakin/periplakin-specific autoantibodies is not yet available.

Methods: Overlapping fragments spanning envoplakin and periplakin, respectively, were analyzed for their ability to bind PNP-specific autoantibodies by immunoblotting and ELISA in sera from patients with PNP (n = 31), pemphigus vulgaris (n = 30), and bullous pemphigoid (n = 50) as well as healthy volunteers (n = 140). The results were compared with those obtained by immunoblotting of extract of cultured human keratinocytes.

Results: Immunoblot analysis revealed that most sera contained antibodies against the N-termini of both plakins as well as against the C-terminus of envoplakin. By ELISA, reactivities against envoplakin(1-481). envoplakin(1626-2033), and periplakin(1-324) were found in 25 (80.6%), 25 (80.6%), and 23 (74.2%) PNP sera, and in 1 (1.2%),3 (3.7%). and 2 (2.5%) control sera, respectively.

Conclusions: The new ELISA based on an N-terminal fragment of envoplakin shows a high diagnostic accuracy to detect circulating autoantibodies in PNP. It is easy to be setup and standardized and can help to differentiate PNP patients from those with pemphigus vulgaris. (C) 2009 Elsevier B.V. All rights reserved.

语种: 英语
所属项目编号: DFG EXC306/1
项目资助者: Werner und Klara Kreitz-Stiftung, Lubeck
WOS记录号: WOS:000272278900002
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56130
Appears in Collections:北京大学第一临床医学院_皮肤性病科_期刊论文

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作者单位: 1.Kurume Univ, Sch Med, Dept Dermatol, Kurume, Fukuoka 830, Japan
2.Med Univ Lubeck, Dept Dermatol, D-23538 Lubeck, Germany
3.EUROIMMUN AG, Inst Expt Immunol, Dept Immunobiochem Res, D-23560 Lubeck, Germany
4.Peking Univ, Hosp 1, Dept Dermatol, Beijing 100871, Peoples R China

Recommended Citation:
Probst, Christian,Schlumberger, Wolfgang,Stoecker, Winfried,et al. Development of ELISA for the specific determination of autoantibodies against envoplakin and periplakin in paraneoplastic pemphigus[J]. CLINICA CHIMICA ACTA,2009,410(1-2):13-18.
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