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Development of ELISA for the specific determination of autoantibodies against envoplakin and periplakin in paraneoplastic pemphigus
Probst, Christian1; Schlumberger, Wolfgang1; Stoecker, Winfried1; Recke, Andreas2; Schmidt, Enno2; Hashimoto, Takashi3; Zhu, Xue Jun4; Zillikens, Detlef2; Komorowski, Lars1
关键词Desmosome Diagnosis Autoantibody Immunofluorescence Microscopy
刊名CLINICA CHIMICA ACTA
2009-12-08
DOI10.1016/j.cca.2009.08.022
410期:1-2页:13-18
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medical Laboratory Technology
资助者Werner und Klara Kreitz-Stiftung, Lubeck ; Werner und Klara Kreitz-Stiftung, Lubeck
研究领域[WOS]Medical Laboratory Technology
关键词[WOS]CORNIFIED ENVELOPE ; ANTI-ENVOPLAKIN ; PLAKIN FAMILY ; DESMOPLAKIN ; ANTIBODIES ; VULGARIS ; EPITOPES ; DOMAIN ; ASSAY ; SERA
英文摘要

Introduction: In paraneoplastic pemphigus (PNP), indirect immunofluorescence microscopy using rat bladder sections is widely used to search for circulating autoantibodies which are predominantly directed against envoplakin and periplakin. A sensitive and specific detection system for envoplakin/periplakin-specific autoantibodies is not yet available.

Methods: Overlapping fragments spanning envoplakin and periplakin, respectively, were analyzed for their ability to bind PNP-specific autoantibodies by immunoblotting and ELISA in sera from patients with PNP (n = 31), pemphigus vulgaris (n = 30), and bullous pemphigoid (n = 50) as well as healthy volunteers (n = 140). The results were compared with those obtained by immunoblotting of extract of cultured human keratinocytes.

Results: Immunoblot analysis revealed that most sera contained antibodies against the N-termini of both plakins as well as against the C-terminus of envoplakin. By ELISA, reactivities against envoplakin(1-481). envoplakin(1626-2033), and periplakin(1-324) were found in 25 (80.6%), 25 (80.6%), and 23 (74.2%) PNP sera, and in 1 (1.2%),3 (3.7%). and 2 (2.5%) control sera, respectively.

Conclusions: The new ELISA based on an N-terminal fragment of envoplakin shows a high diagnostic accuracy to detect circulating autoantibodies in PNP. It is easy to be setup and standardized and can help to differentiate PNP patients from those with pemphigus vulgaris. (C) 2009 Elsevier B.V. All rights reserved.

语种英语
所属项目编号DFG EXC306/1
资助者Werner und Klara Kreitz-Stiftung, Lubeck ; Werner und Klara Kreitz-Stiftung, Lubeck
WOS记录号WOS:000272278900002
引用统计
被引频次:45[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56130
专题北京大学第一临床医学院_皮肤性病科
作者单位1.Kurume Univ, Sch Med, Dept Dermatol, Kurume, Fukuoka 830, Japan
2.Med Univ Lubeck, Dept Dermatol, D-23538 Lubeck, Germany
3.EUROIMMUN AG, Inst Expt Immunol, Dept Immunobiochem Res, D-23560 Lubeck, Germany
4.Peking Univ, Hosp 1, Dept Dermatol, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Probst, Christian,Schlumberger, Wolfgang,Stoecker, Winfried,et al. Development of ELISA for the specific determination of autoantibodies against envoplakin and periplakin in paraneoplastic pemphigus[J]. CLINICA CHIMICA ACTA,2009,410(1-2):13-18.
APA Probst, Christian.,Schlumberger, Wolfgang.,Stoecker, Winfried.,Recke, Andreas.,Schmidt, Enno.,...&Komorowski, Lars.(2009).Development of ELISA for the specific determination of autoantibodies against envoplakin and periplakin in paraneoplastic pemphigus.CLINICA CHIMICA ACTA,410(1-2),13-18.
MLA Probst, Christian,et al."Development of ELISA for the specific determination of autoantibodies against envoplakin and periplakin in paraneoplastic pemphigus".CLINICA CHIMICA ACTA 410.1-2(2009):13-18.
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