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学科主题: 临床医学
题名:
Circulating DNA of HOTAIR in serum is a novel biomarker for breast cancer
作者: Zhang, Lei1; Song, Xinyun1; Wang, Xiaoxia1; Xie, Yuntao2; Wang, Zengwu3; Xu, Ye2; You, Xin4; Liang, Zicai1; Cao, Huiqing1
关键词: HOTAIR ; Circulating DNA ; Breast cancer ; Diagnosis
刊名: BREAST CANCER RESEARCH AND TREATMENT
发表日期: 2015-07-01
DOI: 10.1007/s10549-015-3431-2
卷: 152, 期:1, 页:199-208
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: LONG NONCODING RNA ; CELL-FREE DNA ; TUMOR DNA ; MICRORNA SIGNATURES ; PROGNOSTIC MARKER ; PROSTATE-CANCER ; GENE-EXPRESSION ; LIQUID BIOPSY ; IN-VIVO ; SURVIVAL
英文摘要:

Long non-coding HOX transcript antisense intergenic RNA (HOTAIR) plays an important role in breast cancer. The purpose of this study was to determine whether circulating HOTAIR can be used for breast cancer diagnosis. HOTAIR in serum was measured by PCR-based direct detection. Reverse transcriptase and DNase I treatment were used to distinguish the DNA and RNA forms of HOTAIR. To determine whether circulating HOTAIR is a biomarker for breast cancer, the DNA of HOTAIR from breast cancer patients and healthy controls was measured at both the discovery stage (48 individuals) and an independent validation stage (156 individuals). The diagnostic accuracy was assessed by the receiver operating characteristic curve (ROC) and the area under the curve (AUC). We showed that the major form of HOTAIR-derived fragment in serum is DNA rather than RNA in our study, the same as for MALAT-1, another well-described lincRNA. A higher circulating DNA level of HOTAIR was found in patients at the discovery stage (P = 0.0008). ROC analysis revealed that the circulating HOTAIR DNA distinguished breast cancer patients from healthy individuals (AUC = 0.799). This finding was confirmed at the validation stage. Though circulating MALAT-1 DNA was altered in the discovery stage, it showed no significant difference in the validation stage. In the entire set of 204 samples, the circulating HOTAIR DNA showed a 2.15-fold change in patients compared with healthy controls (P < 0.0001, AUC = 0.786). The optimal cutoff value for diagnosis was 0.30 with sensitivity of 80.0 % and specificity of 68.3 %. Moreover, a correlation between the DNA level of circulating HOTAIR and the progress of breast cancer was established. We have demonstrated that the circulating DNA of HOTAIR is a potential biomarker for breast cancer.

语种: 英语
所属项目编号: 2012AA022501 ; 81170097
项目资助者: National High-Tech R&amp ; D Program of China ; National Natural Science Foundation of China ; 985 Project of Peking University
WOS记录号: WOS:000356248900021
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56134
Appears in Collections:北京大学临床肿瘤学院_乳腺癌预防治疗中心_期刊论文

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作者单位: 1.Peking Univ, Inst Mol Med, Beijing 100871, Peoples R China
2.Peking Univ, Canc Hosp & Inst, Key Lab Carcinogenesis & Translat Res, Breast Ctr, Beijing 100871, Peoples R China
3.Beijing Fuwai Hosp, Div Prevent & Community Hlth, Natl Ctr Cardiovasc Dis, Beijing, Peoples R China
4.Yanbian Univ, Dept Lab Med, Affiliated Hosp, Yanji, Peoples R China

Recommended Citation:
Zhang, Lei,Song, Xinyun,Wang, Xiaoxia,et al. Circulating DNA of HOTAIR in serum is a novel biomarker for breast cancer[J]. BREAST CANCER RESEARCH AND TREATMENT,2015,152(1):199-208.
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