IR@PKUHSC  > 北京大学药学院  > 化学生物学系
学科主题药学
Qualitative and Quantitative Determination of Oligonucleotides by Non-Gel Capillary Electrophoresis
Li, Wujie1; Ma, Yinling1; Gan, Zhu2; Ling, Xiaomei1,2; Yang, Zhenjun2
关键词Non-gel Capillary Electrophoresis Polyethylene Glycol (Peg) 35000 Oligonucleotide
刊名CHROMATOGRAPHIA
2011-03-01
DOI10.1007/s10337-010-1907-0
73期:5-6页:579-582
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
资助者National Natural Science Foundation of China ; Natural Science Foundation of Beijing ; Open Foundation of the State Key Laboratory of Natural and Biomimetic Drugs ; National New Drug Research and Development Project of China ; National High-Tech Research and Development Program of China (863 Program) ; National Natural Science Foundation of China ; Natural Science Foundation of Beijing ; Open Foundation of the State Key Laboratory of Natural and Biomimetic Drugs ; National New Drug Research and Development Project of China ; National High-Tech Research and Development Program of China (863 Program)
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]PERFORMANCE ; SEPARATION ; MATRICES ; COLUMN ; CE
英文摘要

A novel simple non-gel capillary electrophoresis (NGCE) method was developed for the qualitative and quantitative determination of oligonucleotides (ODNs). ODNs were synthesized in a DNA synthesizer and separated by NGCE under optimized conditions as follows: 25 mmol L(-1) Tris-boric-EDTA buffer (pH 8.0) with 7 mmol L(-1) urea in the presence of 20% (w/v) polyethylene glycol (PEG) 35000 at 30 A degrees C and -20 kV. Single-base differences, in which there are differences in the base quantity and the single-base structure with the same base quantity, can be identified by the proposed method, and the quantitative determination was carried out. The obtained regression equation revealed a good linear relationship between the peak area ratio and the ODNs concentration. The correlation coefficient was 0.9986 and the relative standard deviation (RSD) of the precision was < 3.6% (intro-day) and < 6.2% (inter-day), respectively. The recoveries of three concentrations (high, middle, and low) were between 99.6% and 107.6%. The established method is sensitive, simple, economical, and can be used in gene mutation detection, pharmacokinetics study of nucleic acid drugs, and so on.

语种英语
所属项目编号81072612 ; 20472006 ; 7102107 ; K20090207 ; 2009ZX09301-010 ; 2006AA02Z144
资助者National Natural Science Foundation of China ; Natural Science Foundation of Beijing ; Open Foundation of the State Key Laboratory of Natural and Biomimetic Drugs ; National New Drug Research and Development Project of China ; National High-Tech Research and Development Program of China (863 Program) ; National Natural Science Foundation of China ; Natural Science Foundation of Beijing ; Open Foundation of the State Key Laboratory of Natural and Biomimetic Drugs ; National New Drug Research and Development Project of China ; National High-Tech Research and Development Program of China (863 Program)
WOS记录号WOS:000288023700020
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56135
Collection北京大学药学院_化学生物学系
作者单位1.Peking Univ, Sch Pharmaceut Sci, Dept Pharmaceut Anal, Beijing 100191, Peoples R China
2.Peking Univ, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
Recommended Citation
GB/T 7714
Li, Wujie,Ma, Yinling,Gan, Zhu,et al. Qualitative and Quantitative Determination of Oligonucleotides by Non-Gel Capillary Electrophoresis[J]. CHROMATOGRAPHIA,2011,73(5-6):579-582.
APA Li, Wujie,Ma, Yinling,Gan, Zhu,Ling, Xiaomei,&Yang, Zhenjun.(2011).Qualitative and Quantitative Determination of Oligonucleotides by Non-Gel Capillary Electrophoresis.CHROMATOGRAPHIA,73(5-6),579-582.
MLA Li, Wujie,et al."Qualitative and Quantitative Determination of Oligonucleotides by Non-Gel Capillary Electrophoresis".CHROMATOGRAPHIA 73.5-6(2011):579-582.
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