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Simultaneous analysis of coumarins and secoiridoids in Cortex Fraxini by high-performance liquid chromatography-diode array detection-electrospray ionization tandem mass spectrometry
Zhou, Lei; Kang, Jie; Fan, Li; Ma, Xiao-Chi; Zhao, Hai-Yu; Han, Jian; Wang, Bao-rong; Guo, De-An
关键词Hplc-dad-esi-ms Cortex Fraxini Coumarins Secoiridoids Quantification Quality Evaluation
刊名JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
2008-05-12
DOI10.1016/j.jpba.2007.12.009
47期:1页:39-46
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Analytical ; Pharmacology & Pharmacy
研究领域[WOS]Chemistry ; Pharmacology & Pharmacy
关键词[WOS]PHENOLIC-COMPOUNDS ; CAPILLARY-ELECTROPHORESIS ; OLIVE OILS ; AESCULIN ; ESCULIN ; BARK ; ESCULETIN ; EXTRACTS
英文摘要

A high-performance liquid chromatography-diode array UV detection-electrospray ionization tandem mass spectrometry (HPLC-DAD-ESI-MS) method was developed and validated for the simultaneous analysis of seven major constituents in Cortex Fraxini, including esculin, esculetin, fraxetin, fraxin, escuside, oleuropein and ligustroside. The contents of the seven constituents were determined by using HPLC-DAD, and the chemical structures of these constituents were identified by using HPLC-DAD-ESI-MS method. The separation was performed on an Agilent Zorbax SB-C-18 column (250 mm x 4.6 mm i.d., 5 mu m) with gradient elution of acetonitrile and 0.3% aqueous acetic acid within 40 min. Detection was performed at 254 nm. The calibration curves showed good linearity (r(2) > 0.9992). The limits of detection (LOD) ranged from 1.07 to 3.19 ng/ml and limits of quantification (LOQ) ranged from 2.79 to 12.75 ng/ml, respectively. The intra- and inter-day precision was less than 5% and the accuracy was ranging from 96.49% to 103.55%. The recovery of the assay was in the range of 97.61-104.36%. The method was successfully applied to the quantification of the seven constituents in different samples of Cortex Fraxini. The results indicated that the developed method could be considered to be a simple, rapid and reliable method for the quality evaluation of Cortex Fraxini. (C) 2007 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000255849100005
引用统计
被引频次:42[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56138
专题北京大学药学院
北京大学药学院_天然药物与仿生药物国家重点实验室
作者单位Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100083, Peoples R China
推荐引用方式
GB/T 7714
Zhou, Lei,Kang, Jie,Fan, Li,et al. Simultaneous analysis of coumarins and secoiridoids in Cortex Fraxini by high-performance liquid chromatography-diode array detection-electrospray ionization tandem mass spectrometry[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2008,47(1):39-46.
APA Zhou, Lei.,Kang, Jie.,Fan, Li.,Ma, Xiao-Chi.,Zhao, Hai-Yu.,...&Guo, De-An.(2008).Simultaneous analysis of coumarins and secoiridoids in Cortex Fraxini by high-performance liquid chromatography-diode array detection-electrospray ionization tandem mass spectrometry.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,47(1),39-46.
MLA Zhou, Lei,et al."Simultaneous analysis of coumarins and secoiridoids in Cortex Fraxini by high-performance liquid chromatography-diode array detection-electrospray ionization tandem mass spectrometry".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 47.1(2008):39-46.
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