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学科主题: 药学
题名:
Simultaneous analysis of coumarins and secoiridoids in Cortex Fraxini by high-performance liquid chromatography-diode array detection-electrospray ionization tandem mass spectrometry
作者: Zhou, Lei; Kang, Jie; Fan, Li; Ma, Xiao-Chi; Zhao, Hai-Yu; Han, Jian; Wang, Bao-rong; Guo, De-An
关键词: HPLC-DAD-ESI-MS ; Cortex Fraxini ; coumarins ; secoiridoids ; quantification ; quality evaluation
刊名: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
发表日期: 2008-05-12
DOI: 10.1016/j.jpba.2007.12.009
卷: 47, 期:1, 页:39-46
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Analytical ; Pharmacology & Pharmacy
研究领域[WOS]: Chemistry ; Pharmacology & Pharmacy
关键词[WOS]: PHENOLIC-COMPOUNDS ; CAPILLARY-ELECTROPHORESIS ; OLIVE OILS ; AESCULIN ; ESCULIN ; BARK ; ESCULETIN ; EXTRACTS
英文摘要:

A high-performance liquid chromatography-diode array UV detection-electrospray ionization tandem mass spectrometry (HPLC-DAD-ESI-MS) method was developed and validated for the simultaneous analysis of seven major constituents in Cortex Fraxini, including esculin, esculetin, fraxetin, fraxin, escuside, oleuropein and ligustroside. The contents of the seven constituents were determined by using HPLC-DAD, and the chemical structures of these constituents were identified by using HPLC-DAD-ESI-MS method. The separation was performed on an Agilent Zorbax SB-C-18 column (250 mm x 4.6 mm i.d., 5 mu m) with gradient elution of acetonitrile and 0.3% aqueous acetic acid within 40 min. Detection was performed at 254 nm. The calibration curves showed good linearity (r(2) > 0.9992). The limits of detection (LOD) ranged from 1.07 to 3.19 ng/ml and limits of quantification (LOQ) ranged from 2.79 to 12.75 ng/ml, respectively. The intra- and inter-day precision was less than 5% and the accuracy was ranging from 96.49% to 103.55%. The recovery of the assay was in the range of 97.61-104.36%. The method was successfully applied to the quantification of the seven constituents in different samples of Cortex Fraxini. The results indicated that the developed method could be considered to be a simple, rapid and reliable method for the quality evaluation of Cortex Fraxini. (C) 2007 Elsevier B.V. All rights reserved.

语种: 英语
WOS记录号: WOS:000255849100005
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56138
Appears in Collections:北京大学药学院_期刊论文

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作者单位: Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100083, Peoples R China

Recommended Citation:
Zhou, Lei,Kang, Jie,Fan, Li,et al. Simultaneous analysis of coumarins and secoiridoids in Cortex Fraxini by high-performance liquid chromatography-diode array detection-electrospray ionization tandem mass spectrometry[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2008,47(1):39-46.
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