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学科主题: 临床医学
题名:
Effective asymmetry in gap junctional intercellular communication between populations of human normal lung fibroblasts and lung carcinoma cells
作者: Zhang, ZQ; Hu, Y; Wang, BJ; Lin, ZX; Naus, CCG; Nicholson, BJ
刊名: CARCINOGENESIS
发表日期: 2004-04-01
DOI: 10.1093/carcin/bgh036
卷: 25, 期:4, 页:473-482
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: BALB/C 3T3 CELLS ; TRANSFORMED-CELLS ; CONNEXIN GENES ; GROWTH-CONTROL ; CANCER-CELLS ; HELA-CELLS ; FUNCTIONAL EXPRESSION ; NONTRANSFORMED CELLS ; EPITHELIAL-CELLS ; GLIOMA-CELLS
英文摘要:

The dysfunction of homologous and/or heterologous gap junctional intercellular communication (GJIC) has been implicated in tumorigenesis of many kinds of cells. Here we have characterized GJIC and the expression of connexins in six human lung carcinoma cell lines and normal lung fibroblasts (HLF). Compared with HLF, all the carcinoma cells showed reduced or little homologous GJIC. They expressed remarkably reduced connexin(Cx)43 mRNA and variable levels of Cx45 mRNA, but neither Cx43 nor Cx45 protein could be detected. However, using a preloading assay, transfer of calcein was observed between donor HLF cells and first order neighboring recipient tumor cells (recipient cells in 1000-fold excess). Transfer from tumor to HLF cells under the same conditions was not seen, although increasing the ratio of donor tumor cells to recipient HLF cells and plating the cells at low density did reveal weak transfer from tumor cells to HLF. Transfection of Cx43 into giant cell carcinoma PG cells increased homologous communication and eliminated the rectifying behavior of heterologous communication. This indicates that the apparent rectification of dye transfer between normal and tumor cells was a product of low rates of heterologous transfer linked to (i) rapid dilution of the dye to below detectable limits through a very well coupled cell population (tumor to HLF) and (ii) concentration of dye in immediate neighbors in a poorly coupled cell population (HLF to tumor cells). These results suggest that the coupling levels may need to exceed a certain threshold to allow propagation of signals over a sufficient distance to affect behavior of a cell population. We propose that the relative rates of heterologous and homologous coupling of cell populations and the ′pool size′ of shared metabolites in tumor cells and the surrounding normal tissue are likely to be very important in the regulation of their growth.

语种: 英语
WOS记录号: WOS:000220485700002
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56144
Appears in Collections:北京大学临床肿瘤学院_细胞生物学研究室_期刊论文

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作者单位: 1.Beijing Univ, Sch Oncol, Beijing Inst Canc Res, Dept Cell Biol, Beijing 100034, Peoples R China
2.Univ British Columbia, Dept Anat & Cell Biol, Vancouver, BC V6T 1Z3, Canada
3.SUNY Buffalo, Dept Biol Sci, Buffalo, NY 14260 USA

Recommended Citation:
Zhang, ZQ,Hu, Y,Wang, BJ,et al. Effective asymmetry in gap junctional intercellular communication between populations of human normal lung fibroblasts and lung carcinoma cells[J]. CARCINOGENESIS,2004,25(4):473-482.
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