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Metabolic and pharmacokinetic studies of curcumin, demethoxycurcumin and bisdemethoxycurcumin in mice tumor after intragastric administration of nanoparticle formulations by liquid chromatography coupled with tandem mass spectrometry
Li, Rui1; Qiao, Xue1; Li, Qingyan2; He, Rong3; Ye, Min1; Xiang, Cheng1; Lin, Xionghao1; Guo, Dean1
关键词Curcumin Curcuminoids Lc/ms/ms Nanoparticle Pharmacokinetics Tumor
刊名JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
2011-09-15
DOI10.1016/j.jchromb.2011.07.042
879期:26页:2751-2758
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]FACTOR-KAPPA-B ; IN-VITRO ; GENE-EXPRESSION ; CELL LINES ; SUPPRESSION ; PATHWAY ; KINASE ; PLASMA ; DIFERULOYLMETHANE ; ACTIVATION
英文摘要

This paper aims to investigate the metabolism and pharmacokinetics of curcumin, demethoxycurcumin and bisdemethoxycurcumin in mice tumor. To improve water solubility, nanoparticle formulations were prepared as curcuminoids-loaded solid lipid nanoparticles (curcuminoicls-SLNs) and curcumin-loaded solid lipid nanoparticles (curcumin-SLNs). After intragastric administration to tumor-bearing ICR mice, the plasma and tumor samples were analyzed by liquid chromatography with ion trap mass spectrometry. We discovered that curcuminoids were mainly present as glucuronides in plasma, whereas in free form in tumor tissue. A validated LC/MS/MS method was established to determine the three free curcuminoids in tumor homogenate. Samples were separated on a Zorbax SB-C(18) column, eluted with acetonitrile-water (containing 0.1% formic acid), and detected by TSQ Quantum triple quadrupole mass spectrometer in selected reaction monitoring mode. The method showed good linearity (r(2) = 0.997-0.999) over wide dynamic ranges (2-6000 ng/mL). Variations within- and between-batch never exceeded 11.2% and 13.4%, respectively. The extraction recovery rates ranged from 78.3% to 87.7%. The pharmacokinetics of curcuminoids in mice tumor fit two-compartment model and first order elimination. For curcumin-SLNs group, the dosing of 250 mg/kg of curcumin resulted in AUC((0-48h)) of 2285 ng h/mL and C(max) of 209 ng/mL. For curcuminoids-SLNs group, the dosing equivalent to 138 mg/kg of curcumin resulted in higher tumor concentrations (AUC= 2811 ng h/mL. C(max) = 285 ng/mL). It appeared that co-existing curcuminoids improved the bioavailability of curcumin. (C) 2011 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000295297800011
项目编号985-2-119-121 ; 2009ZX09304-002
资助机构Peking University ; Ministry of Science and Technology of China
引用统计
被引频次:28[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56153
专题北京大学药学院
北京大学药学院_天然药物学系
北京大学第一临床医学院_手术室
北京大学第三临床医学院_心血管内科
作者单位1.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
2.Civil Aviat Adm China, Civil Aviat Med Ctr, Beijing 100123, Peoples R China
3.Peking Univ Third Hosp, Dept Cardiol, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Li, Rui,Qiao, Xue,Li, Qingyan,et al. Metabolic and pharmacokinetic studies of curcumin, demethoxycurcumin and bisdemethoxycurcumin in mice tumor after intragastric administration of nanoparticle formulations by liquid chromatography coupled with tandem mass spectrometry[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2011,879(26):2751-2758.
APA Li, Rui.,Qiao, Xue.,Li, Qingyan.,He, Rong.,Ye, Min.,...&Guo, Dean.(2011).Metabolic and pharmacokinetic studies of curcumin, demethoxycurcumin and bisdemethoxycurcumin in mice tumor after intragastric administration of nanoparticle formulations by liquid chromatography coupled with tandem mass spectrometry.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,879(26),2751-2758.
MLA Li, Rui,et al."Metabolic and pharmacokinetic studies of curcumin, demethoxycurcumin and bisdemethoxycurcumin in mice tumor after intragastric administration of nanoparticle formulations by liquid chromatography coupled with tandem mass spectrometry".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 879.26(2011):2751-2758.
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