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学科主题: 药学
题名:
Metabolic and pharmacokinetic studies of curcumin, demethoxycurcumin and bisdemethoxycurcumin in mice tumor after intragastric administration of nanoparticle formulations by liquid chromatography coupled with tandem mass spectrometry
作者: Li, Rui1; Qiao, Xue1; Li, Qingyan2; He, Rong3; Ye, Min1; Xiang, Cheng1; Lin, Xionghao1; Guo, Dean1
关键词: Curcumin ; Curcuminoids ; LC/MS/MS ; Nanoparticle ; Pharmacokinetics ; Tumor
刊名: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
发表日期: 2011-09-15
DOI: 10.1016/j.jchromb.2011.07.042
卷: 879, 期:26, 页:2751-2758
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: FACTOR-KAPPA-B ; IN-VITRO ; GENE-EXPRESSION ; CELL LINES ; SUPPRESSION ; PATHWAY ; KINASE ; PLASMA ; DIFERULOYLMETHANE ; ACTIVATION
英文摘要:

This paper aims to investigate the metabolism and pharmacokinetics of curcumin, demethoxycurcumin and bisdemethoxycurcumin in mice tumor. To improve water solubility, nanoparticle formulations were prepared as curcuminoids-loaded solid lipid nanoparticles (curcuminoicls-SLNs) and curcumin-loaded solid lipid nanoparticles (curcumin-SLNs). After intragastric administration to tumor-bearing ICR mice, the plasma and tumor samples were analyzed by liquid chromatography with ion trap mass spectrometry. We discovered that curcuminoids were mainly present as glucuronides in plasma, whereas in free form in tumor tissue. A validated LC/MS/MS method was established to determine the three free curcuminoids in tumor homogenate. Samples were separated on a Zorbax SB-C(18) column, eluted with acetonitrile-water (containing 0.1% formic acid), and detected by TSQ Quantum triple quadrupole mass spectrometer in selected reaction monitoring mode. The method showed good linearity (r(2) = 0.997-0.999) over wide dynamic ranges (2-6000 ng/mL). Variations within- and between-batch never exceeded 11.2% and 13.4%, respectively. The extraction recovery rates ranged from 78.3% to 87.7%. The pharmacokinetics of curcuminoids in mice tumor fit two-compartment model and first order elimination. For curcumin-SLNs group, the dosing of 250 mg/kg of curcumin resulted in AUC((0-48h)) of 2285 ng h/mL and C(max) of 209 ng/mL. For curcuminoids-SLNs group, the dosing equivalent to 138 mg/kg of curcumin resulted in higher tumor concentrations (AUC= 2811 ng h/mL. C(max) = 285 ng/mL). It appeared that co-existing curcuminoids improved the bioavailability of curcumin. (C) 2011 Elsevier B.V. All rights reserved.

语种: 英语
所属项目编号: 985-2-119-121 ; 2009ZX09304-002
项目资助者: Peking University ; Ministry of Science and Technology of China
WOS记录号: WOS:000295297800011
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56153
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
2.Civil Aviat Adm China, Civil Aviat Med Ctr, Beijing 100123, Peoples R China
3.Peking Univ Third Hosp, Dept Cardiol, Beijing 100191, Peoples R China

Recommended Citation:
Li, Rui,Qiao, Xue,Li, Qingyan,et al. Metabolic and pharmacokinetic studies of curcumin, demethoxycurcumin and bisdemethoxycurcumin in mice tumor after intragastric administration of nanoparticle formulations by liquid chromatography coupled with tandem mass spectrometry[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2011,879(26):2751-2758.
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