IR@PKUHSC  > 北京大学基础医学院  > 病理学系
学科主题基础医学
HECT ubiquitin ligase Smurf1 targets the tumor suppressor ING2 for ubiquitination and degradation
Nie, Jing2; Liu, Lin3; Wu, Min4,5; Xing, Guichun2; He, Shan6; Yin, Yuxin7; Tian, Chunyan1,2; He, Fuchu2; Zhang, Lingqiang2,4,5
关键词Inhibitor of growth 2 Tumor suppressor Smad ubiquitination regulatory factor 1 Ubiquitination Protein degradation
刊名FEBS LETTERS
2010-07-16
DOI10.1016/j.febslet.2010.05.033
584期:14页:3005-3012
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
关键词[WOS]CELL POLARITY ; GENE FAMILY ; PHD FINGER ; EXPRESSION ; ACETYLATION ; APOPTOSIS ; PROTEINS ; RECEPTOR ; PATHWAY ; CANCER
英文摘要

Inhibitor of growth 2 (ING2) gene encodes a candidate tumor suppressor and is frequently reduced in many tumors. However, the mechanisms underlying the regulation of ING2, in particular its protein stability, are still unclear. Here we show that the homologous to E6AP carboxyl terminus (HECT)-type ubiquitin ligase Smad ubiquitination regulatory factor 1 (Smurf1) interacts with and targets ING2 for poly-ubiquitination and proteasomal degradation. Intriguingly, the ING2 binding domain in Smurf1 was mapped to the catalytic HECT domain. Furthermore, the C-terminal PHD domain of ING2 was required for Smurf1-mediated degradation. This study provided the first evidence that the stability of ING2 could be regulated by ubiquitin-mediated degradation.

Structured summary:

MINT-7894271: ING2 (uniprotkb:Q9H160) binds (MI:0407) to Smurf1 (uniprotkb:Q9HCE7) by pull-down (MI:0096)

MINT-7894319, MINT-7894339: ING2 (uniprotkb:Q9H160) physically interacts (MI:0915) with Smurf1 (uniprotkb:Q9HCE7) by anti tag co-immunoprecipitation (MI:0007)

MINT-7894301: Smurf1 (uniprotkb:Q9HCE7) physically interacts (MI:0915) with ING2 (uniprotkb: Q9H160) by anti bait co-immunoprecipitation (MI:0006)

MINT-7894358: ING1b (uniprotkb:Q9UK53-2) physically interacts (MI:0915) with Smurf1 (uniprotkb: Q9HCE7) by anti tag co-immunoprecipitation (MI:0007)

MINT-7894249: ING2 (uniprotkb:Q9H160) physically interacts (MI:0915) with ubiquitin (uniprotkb: P62988) by anti tag co-immunoprecipitation (MI:0007) (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000279438800008
引用统计
被引频次:22[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56155
专题北京大学基础医学院_病理学系
北京大学基础医学院
作者单位1.Beijing Inst Radiat Med, Dept Genom & Prote, Beijing Prote Res Ctr, State Key Lab Prote, Beijing, Peoples R China
2.Tsinghua Univ, Dept Biol Sci & Biotechnol, Beijing 100084, Peoples R China
3.Gen Hosp Chinese PLA, Dept Gen Surg, Beijing, Peoples R China
4.Anhui Med Univ, Mol Biol Lab, Hefei, Anhui, Peoples R China
5.Anhui Med Univ, Dept Biochem, Key Lab Gene Resource Utilizat Severe Dis, Hefei, Anhui, Peoples R China
6.Beijing Univ Technol, Coll Life Sci & Bioengn, Beijing, Peoples R China
7.Peking Univ, Dept Pathol, Sch Basic Med Sci, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Nie, Jing,Liu, Lin,Wu, Min,et al. HECT ubiquitin ligase Smurf1 targets the tumor suppressor ING2 for ubiquitination and degradation[J]. FEBS LETTERS,2010,584(14):3005-3012.
APA Nie, Jing.,Liu, Lin.,Wu, Min.,Xing, Guichun.,He, Shan.,...&Zhang, Lingqiang.(2010).HECT ubiquitin ligase Smurf1 targets the tumor suppressor ING2 for ubiquitination and degradation.FEBS LETTERS,584(14),3005-3012.
MLA Nie, Jing,et al."HECT ubiquitin ligase Smurf1 targets the tumor suppressor ING2 for ubiquitination and degradation".FEBS LETTERS 584.14(2010):3005-3012.
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