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学科主题: 基础医学
题名:
HECT ubiquitin ligase Smurf1 targets the tumor suppressor ING2 for ubiquitination and degradation
作者: Nie, Jing2; Liu, Lin3; Wu, Min4,5; Xing, Guichun2; He, Shan6; Yin, Yuxin7; Tian, Chunyan1,2; He, Fuchu2; Zhang, Lingqiang2,4,5
关键词: Inhibitor of growth 2 ; Tumor suppressor ; Smad ubiquitination regulatory factor 1 ; Ubiquitination ; Protein degradation
刊名: FEBS LETTERS
发表日期: 2010-07-16
DOI: 10.1016/j.febslet.2010.05.033
卷: 584, 期:14, 页:3005-3012
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
关键词[WOS]: CELL POLARITY ; GENE FAMILY ; PHD FINGER ; EXPRESSION ; ACETYLATION ; APOPTOSIS ; PROTEINS ; RECEPTOR ; PATHWAY ; CANCER
英文摘要:

Inhibitor of growth 2 (ING2) gene encodes a candidate tumor suppressor and is frequently reduced in many tumors. However, the mechanisms underlying the regulation of ING2, in particular its protein stability, are still unclear. Here we show that the homologous to E6AP carboxyl terminus (HECT)-type ubiquitin ligase Smad ubiquitination regulatory factor 1 (Smurf1) interacts with and targets ING2 for poly-ubiquitination and proteasomal degradation. Intriguingly, the ING2 binding domain in Smurf1 was mapped to the catalytic HECT domain. Furthermore, the C-terminal PHD domain of ING2 was required for Smurf1-mediated degradation. This study provided the first evidence that the stability of ING2 could be regulated by ubiquitin-mediated degradation.

Structured summary:

MINT-7894271: ING2 (uniprotkb:Q9H160) binds (MI:0407) to Smurf1 (uniprotkb:Q9HCE7) by pull-down (MI:0096)

MINT-7894319, MINT-7894339: ING2 (uniprotkb:Q9H160) physically interacts (MI:0915) with Smurf1 (uniprotkb:Q9HCE7) by anti tag co-immunoprecipitation (MI:0007)

MINT-7894301: Smurf1 (uniprotkb:Q9HCE7) physically interacts (MI:0915) with ING2 (uniprotkb: Q9H160) by anti bait co-immunoprecipitation (MI:0006)

MINT-7894358: ING1b (uniprotkb:Q9UK53-2) physically interacts (MI:0915) with Smurf1 (uniprotkb: Q9HCE7) by anti tag co-immunoprecipitation (MI:0007)

MINT-7894249: ING2 (uniprotkb:Q9H160) physically interacts (MI:0915) with ubiquitin (uniprotkb: P62988) by anti tag co-immunoprecipitation (MI:0007) (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

语种: 英语
所属项目编号: 2007CB914601 ; 2006CB910802 ; 2009CB918402 ; 2010CB912202 ; 30970601 ; 2009ZX09503-002 ; 2009ZX09301-002 ; K200802
项目资助者: Program of China ; National Natural Science Foundation ; National Key Technologies R& ; D Program for New Drugs ; State Key Laboratory of Proteomics
WOS记录号: WOS:000279438800008
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56155
Appears in Collections:基础医学院_病理学系_期刊论文

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作者单位: 1.Beijing Inst Radiat Med, Dept Genom & Prote, Beijing Prote Res Ctr, State Key Lab Prote, Beijing, Peoples R China
2.Tsinghua Univ, Dept Biol Sci & Biotechnol, Beijing 100084, Peoples R China
3.Gen Hosp Chinese PLA, Dept Gen Surg, Beijing, Peoples R China
4.Anhui Med Univ, Mol Biol Lab, Hefei, Anhui, Peoples R China
5.Anhui Med Univ, Dept Biochem, Key Lab Gene Resource Utilizat Severe Dis, Hefei, Anhui, Peoples R China
6.Beijing Univ Technol, Coll Life Sci & Bioengn, Beijing, Peoples R China
7.Peking Univ, Dept Pathol, Sch Basic Med Sci, Beijing 100871, Peoples R China

Recommended Citation:
Nie, Jing,Liu, Lin,Wu, Min,et al. HECT ubiquitin ligase Smurf1 targets the tumor suppressor ING2 for ubiquitination and degradation[J]. FEBS LETTERS,2010,584(14):3005-3012.
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