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Evaluation of five routine glucose methods on an Olympus AU5400 analyzer using the CDC hexokinase reference method
Jia, Ke-ke; Zhang, Jie
关键词Evaluation Glucose Glucose Oxidase Hexokinase Reference Method
刊名CLINICAL CHEMISTRY AND LABORATORY MEDICINE
2010-03-01
DOI10.1515/CCLM.2010.018
48期:3页:361-364
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medical Laboratory Technology
研究领域[WOS]Medical Laboratory Technology
英文摘要

Background: In China, two common methods are used to detect serum glucose, glucose oxidase (GOD) and hexokinase (HK) methods. We evaluated five commercially available glucose reagent kits with the Center of Disease Control (CDC) HK reference method.

Methods: Five routine methods were compared with the HK reference method according to Clinical and Laboratory Standards Institute (CLSI) guideline (EP9-A2). These five routine methods were performed on an "open channel′′ Olympus 5400 analyzer.

Results: All five evaluated methods were closely correlated to the reference method (r > 0.9998). Compared to the HK reference method, the Olympus-HK method and Roche-GOD method showed a very small positive bias (mean +/- SD: 0.08 +/- 0.09 mmol/L and 0.03 +/- 0.08 mmol/L); while the Biosino-HK method, Osborne-GOD method and Baiding-GOD method showed a very small negative bias (mean +/- SD: -0.07 +/- 0.06 mmol/L, -0.09 +/- 0.07 mmol/L and -0.01 +/- 0.04 mmol/L).

Conclusions: All five reagents we evaluated using matching calibrators showed good correlation to the reference method and small bias. These five glucose reagents are acceptable for use in clinical laboratories. Clin Chem Lab Med 2010;48:361-4.

语种英语
WOS记录号WOS:000274725300009
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56157
专题北京大学第三临床医学院
作者单位Peking Univ, Hosp 3, Dept Clin Lab, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Jia, Ke-ke,Zhang, Jie. Evaluation of five routine glucose methods on an Olympus AU5400 analyzer using the CDC hexokinase reference method[J]. CLINICAL CHEMISTRY AND LABORATORY MEDICINE,2010,48(3):361-364.
APA Jia, Ke-ke,&Zhang, Jie.(2010).Evaluation of five routine glucose methods on an Olympus AU5400 analyzer using the CDC hexokinase reference method.CLINICAL CHEMISTRY AND LABORATORY MEDICINE,48(3),361-364.
MLA Jia, Ke-ke,et al."Evaluation of five routine glucose methods on an Olympus AU5400 analyzer using the CDC hexokinase reference method".CLINICAL CHEMISTRY AND LABORATORY MEDICINE 48.3(2010):361-364.
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