IR@PKUHSC  > 北京大学基础医学院  > 病原生物学系
学科主题基础医学
A Dual-Reporter System for Real-Time Monitoring and High-throughput CRISPR/Cas9 Library Screening of the Hepatitis C Virus
Ren, Qingpeng1; Li, Chan1; Yuan, Pengfei1; Cai, Changzu1; Zhang, Linqi2,3; Luo, Guangxiang George4,5; Wei, Wensheng1
刊名SCIENTIFIC REPORTS
2015-03-09
DOI10.1038/srep08865
5期:mar
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]TO-CELL TRANSMISSION ; B TYPE-I ; ENTRY FACTOR ; INFECTION ; PROTEASE ; GENOME ; REPLICATION ; EXPRESSION ; RECEPTOR ; CULTURE
英文摘要

The hepatitis C virus (HCV) is one of the leading causes of chronic hepatitis, liver cirrhosis and hepatocellular carcinomas and infects approximately 170 million people worldwide. Although several reporter systems have been developed, many shortcomings limit their use in the assessment of HCV infections. Here, we report a real-time live-cell reporter, termed the NIrD (NS3-4A Inducible rtTA-mediated Dual-reporter) system, which provides an on-off switch specifically in response to an HCV infection. Using the NIrD system and a focused CRISPR/Cas9 library, we identified CLDN1, OCLN and CD81 as essential genes for both the cell-free entry and the cell-to-cell transmission of HCV. The combination of this ultra-sensitive reporter system and the CRISPR knockout screening provides a powerful and high-throughput strategy for the identification of critical host components for HCV infections.

语种英语
WOS记录号WOS:000350549200015
项目编号2010CB911800 ; NSFC31430025 ; NSFC31170126 ; NSFC81471909
资助机构National Basic Research Program of China ; National Science Foundation of China ; Peking-Tsinghua Center for Life Sciences
引用统计
被引频次:20[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56166
专题北京大学基础医学院_病原生物学系
作者单位1.Peking Univ, Biodynam Opt Imaging Ctr BIOPIC, State Key Lab Prot & Plant Gene Res, Peking Tsinghua Ctr Life Sci,Sch Life Sci, Beijing 100871, Peoples R China
2.Tsinghua Univ, Sch Med, Comprehens AIDS Res Ctr, Beijing 100084, Peoples R China
3.Tsinghua Univ, Sch Med, Res Ctr Publ Hlth, Beijing 100084, Peoples R China
4.Univ Alabama Birmingham, Med Sch Birmingham, Dept Microbiol, Birmingham, AL 35294 USA
5.Peking Univ, Coll Basic Med Sci, Dept Microbiol, Beijing 100083, Peoples R China
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GB/T 7714
Ren, Qingpeng,Li, Chan,Yuan, Pengfei,et al. A Dual-Reporter System for Real-Time Monitoring and High-throughput CRISPR/Cas9 Library Screening of the Hepatitis C Virus[J]. SCIENTIFIC REPORTS,2015,5(mar).
APA Ren, Qingpeng.,Li, Chan.,Yuan, Pengfei.,Cai, Changzu.,Zhang, Linqi.,...&Wei, Wensheng.(2015).A Dual-Reporter System for Real-Time Monitoring and High-throughput CRISPR/Cas9 Library Screening of the Hepatitis C Virus.SCIENTIFIC REPORTS,5(mar).
MLA Ren, Qingpeng,et al."A Dual-Reporter System for Real-Time Monitoring and High-throughput CRISPR/Cas9 Library Screening of the Hepatitis C Virus".SCIENTIFIC REPORTS 5.mar(2015).
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