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学科主题: 基础医学
题名:
A Dual-Reporter System for Real-Time Monitoring and High-throughput CRISPR/Cas9 Library Screening of the Hepatitis C Virus
作者: Ren, Qingpeng1; Li, Chan1; Yuan, Pengfei1; Cai, Changzu1; Zhang, Linqi2,3; Luo, Guangxiang George4,5; Wei, Wensheng1
刊名: SCIENTIFIC REPORTS
发表日期: 2015-03-09
DOI: 10.1038/srep08865
卷: 5, 期:mar
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: TO-CELL TRANSMISSION ; B TYPE-I ; ENTRY FACTOR ; INFECTION ; PROTEASE ; GENOME ; REPLICATION ; EXPRESSION ; RECEPTOR ; CULTURE
英文摘要:

The hepatitis C virus (HCV) is one of the leading causes of chronic hepatitis, liver cirrhosis and hepatocellular carcinomas and infects approximately 170 million people worldwide. Although several reporter systems have been developed, many shortcomings limit their use in the assessment of HCV infections. Here, we report a real-time live-cell reporter, termed the NIrD (NS3-4A Inducible rtTA-mediated Dual-reporter) system, which provides an on-off switch specifically in response to an HCV infection. Using the NIrD system and a focused CRISPR/Cas9 library, we identified CLDN1, OCLN and CD81 as essential genes for both the cell-free entry and the cell-to-cell transmission of HCV. The combination of this ultra-sensitive reporter system and the CRISPR knockout screening provides a powerful and high-throughput strategy for the identification of critical host components for HCV infections.

语种: 英语
所属项目编号: 2010CB911800 ; NSFC31430025 ; NSFC31170126 ; NSFC81471909
项目资助者: National Basic Research Program of China ; National Science Foundation of China ; Peking-Tsinghua Center for Life Sciences
WOS记录号: WOS:000350549200015
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56166
Appears in Collections:基础医学院_病原生物学系_期刊论文

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作者单位: 1.Peking Univ, Biodynam Opt Imaging Ctr BIOPIC, State Key Lab Prot & Plant Gene Res, Peking Tsinghua Ctr Life Sci,Sch Life Sci, Beijing 100871, Peoples R China
2.Tsinghua Univ, Sch Med, Comprehens AIDS Res Ctr, Beijing 100084, Peoples R China
3.Tsinghua Univ, Sch Med, Res Ctr Publ Hlth, Beijing 100084, Peoples R China
4.Univ Alabama Birmingham, Med Sch Birmingham, Dept Microbiol, Birmingham, AL 35294 USA
5.Peking Univ, Coll Basic Med Sci, Dept Microbiol, Beijing 100083, Peoples R China

Recommended Citation:
Ren, Qingpeng,Li, Chan,Yuan, Pengfei,et al. A Dual-Reporter System for Real-Time Monitoring and High-throughput CRISPR/Cas9 Library Screening of the Hepatitis C Virus[J]. SCIENTIFIC REPORTS,2015,5(mar).
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