IR@PKUHSC  > 北京大学第二临床医学院  > 重症医学科
学科主题临床医学
Study on Invadopodia Formation for Lung Carcinoma Invasion with a Microfluidic 3D Culture Device
Wang, Shanshan1; Li, Encheng1; Gao, Yanghui1; Wang, Yan1; Guo, Zhe1; He, Jiarui1; Zhang, Jianing2; Gao, Zhancheng3,4; Wang, Qi5
刊名PLOS ONE
2013-02-18
DOI10.1371/journal.pone.0056448
8期:2
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
资助者National High-tech R&amp ; D Program (973 Program) ; National Natural Science Foundation of China ; National High-tech R&amp ; D Program (973 Program) ; National Natural Science Foundation of China
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]GROWTH-FACTOR EXPRESSION ; ON-A-CHIP ; CANCER-CELLS ; TUMOR-CELLS ; MATRIX METALLOPROTEINASES ; MOLECULAR-MECHANISMS ; PERFUSION-CULTURE ; MAMMALIAN-CELLS ; N-WASP ; MIGRATION
英文摘要

Invadopodia or invasive feet, which are actin-rich membrane protrusions with matrix degradation activity formed by invasive cancer cells, are a key determinant in the malignant invasive progression of tumors and represent an important target for cancer therapies. In this work, we presented a microfluidic 3D culture device with continuous supplement of fresh media via a syringe pump. The device mimicked tumor microenvironment in vivo and could be used to assay invadopodia formation and to study the mechanism of human lung cancer invasion. With this device, we investigated the effects of epidermal growth factor (EGF) and matrix metalloproteinase (MMP) inhibitor, GM6001 on invadopodia formation by human non-small cell lung cancer cell line A549 in 3D matrix model. This device was composed of three units that were capable of achieving the assays on one control group and two experimental groups′ cells, which were simultaneously pretreated with EGF or GM6001 in parallel. Immunofluorescence analysis of invadopodia formation and extracellular matrix degradation was conducted using confocal imaging system. We observed that EGF promoted invadopodia formation by A549 cells in 3D matrix and that GM6001 inhibited the process. These results demonstrated that epidermal growth factor receptor (EGFR) signaling played a significant role in invadopodia formation and related ECM degradation activity. Meanwhile, it was suggested that MMP inhibitor (GM6001) might be a powerful therapeutic agent targeting invadopodia formation in tumor invasion. This work clearly demonstrated that the microfluidic-based 3D culture device provided an applicable platform for elucidating the mechanism of cancer invasion and could be used in testing other anti-invasion agents.

语种英语
所属项目编号2012CB822103 ; 91129733 ; 81071228
资助者National High-tech R&amp ; D Program (973 Program) ; National Natural Science Foundation of China ; National High-tech R&amp ; D Program (973 Program) ; National Natural Science Foundation of China
WOS记录号WOS:000315159200052
Citation statistics
Cited Times:15[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56188
Collection北京大学第二临床医学院_重症医学科
作者单位1.Dalian Med Univ, Grad Sch, Dalian, Peoples R China
2.Peking Univ Peoples Hosp, Dept Resp, Beijing, Peoples R China
3.Dalian Med Univ, Inst Glycobiol, Dept Biochem, Dalian, Peoples R China
4.Peking Univ Peoples Hosp, Dept Crit Care Med, Beijing, Peoples R China
5.Dalian Med Univ, Hosp 2, Dept Resp Med, Dalian, Peoples R China
Recommended Citation
GB/T 7714
Wang, Shanshan,Li, Encheng,Gao, Yanghui,et al. Study on Invadopodia Formation for Lung Carcinoma Invasion with a Microfluidic 3D Culture Device[J]. PLOS ONE,2013,8(2).
APA Wang, Shanshan.,Li, Encheng.,Gao, Yanghui.,Wang, Yan.,Guo, Zhe.,...&Wang, Qi.(2013).Study on Invadopodia Formation for Lung Carcinoma Invasion with a Microfluidic 3D Culture Device.PLOS ONE,8(2).
MLA Wang, Shanshan,et al."Study on Invadopodia Formation for Lung Carcinoma Invasion with a Microfluidic 3D Culture Device".PLOS ONE 8.2(2013).
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