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学科主题: 临床医学
题名:
Derivation and characterization of Chinese human embryonic stem cell line with high potential to differentiate into pancreatic and hepatic cells
作者: Shi Cheng2; Shen Huan2; Jiang Wei3; Song Zhi-hua3; Wang Cheng-yan3; We Li-hui1
关键词: human embryonic stem cell lines ; blastocysts ; embryonic stem cell isolation ; pancreatic cells ; hepatic cells
刊名: CHINESE MEDICAL JOURNAL
发表日期: 2011-04-05
DOI: 10.3760/cma.j.issn.0366-6999.2011.07.017
卷: 124, 期:7, 页:1037-1043
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, General & Internal
研究领域[WOS]: General & Internal Medicine
关键词[WOS]: HUMAN BLASTOCYSTS ; IN-VITRO ; CARDIOMYOCYTES ; GENERATION
英文摘要:

Background Human embryonic stem cells have prospective uses in regenerative medicine and drug screening. Every human embryonic stem cell line has its own genetic background, which determines its specific ability for differentiation as well as susceptibility to drugs. It is necessary to compile many human embryonic stem cell lines with various backgrounds for future clinical use, especially in China due to its large population. This study contributes to isolating new Chinese human embryonic stem cell lines with clarified directly differentiation ability.

Methods Donated embryos that exceeded clinical use in our in vitro fertilization-embryo transfer (IVF-ET) center were collected to establish human embryonic stem cells lines with informed consent. The classic growth factors of basic fibroblast growth factor (bFGF) and recombinant human leukaemia inhibitory factor (hLIF) for culturing embryonic stem cells were used to capture the stem cells from the plated embryos. Mechanical and enzymetic methods were used to propagate the newly established human embryonic stem cells line. The new cell line was checked for pluripotent characteristics with detecting the expression of sternness genes and observing spontaneous differentiation both in vitro and in vivo. Finally similar step-wise protocols from definitive endoderm to target specific cells were used to check the cell line′s ability to directly differentiate into pancreatic and hepatic cells.

Results We generated a new Chinese human embryonic stem cells line, CH1. This cell line showed the same characteristics as other reported Chinese human embryonic stern cells lines: normal morphology, karyotype and pluripotency in vitro and in vivo. The CH1 cells could be directly differentiated towards pancreatic and hepatic cells with equal efficiency compared to the H1 cell line.

Conclusions This newly established Chinese cell line, CH1, which is pluripotent and has high potential to differentiate into pancreatic and hepatic cells, will provide a useful tool for embryo development research, along with clinical treatments for diabetes and some hepatic diseases. Chin Med J 2011;124(7):1037-1043

语种: 英语
所属项目编号: 2007CB947901
项目资助者: National Basic Research Program of China ("973" Program)
WOS记录号: WOS:000290289700018
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56207
Appears in Collections:北京大学第二临床医学院_期刊论文

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作者单位: 1.Peking Univ, Peking Univ Peoples Hosp, Dept Obstet & Gynecol, Beijing 100044, Peoples R China
2.Peking Univ, Peking Univ Peoples Hosp, Reprod Med Ctr, Beijing 100044, Peoples R China
3.Peking Univ, Coll Life Sci, Lab Stem Cell & Generat Biol, Beijing 100871, Peoples R China

Recommended Citation:
Shi Cheng,Shen Huan,Jiang Wei,et al. Derivation and characterization of Chinese human embryonic stem cell line with high potential to differentiate into pancreatic and hepatic cells[J]. CHINESE MEDICAL JOURNAL,2011,124(7):1037-1043.
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