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学科主题: 临床医学
题名:
Gene transfer of kringle 5 of plasminogen by electroporation inhibits corneal neovascularization
作者: Yu, WZ; Li, XX; She, HC; He, PY; Dong, JQ; Rui, M; Ma, DL
关键词: kringle 5 of plasminogen ; gene therapy ; DNA plasmid ; corneal neovascularization ; electroporation ; rat
刊名: OPHTHALMIC RESEARCH
发表日期: 2003-09-01
DOI: 10.1159/000072143
卷: 35, 期:5, 页:239-246
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Ophthalmology
研究领域[WOS]: Ophthalmology
关键词[WOS]: ENDOTHELIAL GROWTH-FACTOR ; TUMOR-GROWTH ; RETINAL NEOVASCULARIZATION ; RAT CORNEA ; ANGIOGENESIS ; EXPRESSION ; ENDOSTATIN ; VECTOR ; CANCER ; MODEL
英文摘要:

Purpose: To test the efficacy of naked plasmid that expresses human kringle 5 of plasminogen (K5) in suppressing experimental corneal neovascularization in a rat model. Methods: A eukaryotic expression plasmid encoding human K5 (pSecK5) was constructed. COS cells were transiently transfected with pSecK5 using a lipid-based transfection reagent. K5 secretion was confirmed by Western blot analysis. The effect of the secreted K5 on the proliferation of human umbilical vein endothelial cells (HUVECs) was investigated colorimetrically. Forty-three Sprague-Dawley rats were used for a corneal neovascularization suppression experiment. Corneal injury was induced by placing a disk of filter paper (immersed in 1 mol/l NaOH, 3.0 mm in diameter) on the corneal surface for 2 min. The cornea was immediately washed with saline. pSecK5 and empty plasmids were injected subconjunctivally, and square-wave electric pulses were immediately applied to the eyes. The expression of K5 was analyzed by reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry. The extent of corneal neovascularization was evaluated by scores. Results: The constructed plasmid could express itself in COS cells. Conditioned medium from K5-transfected COS cells apparently inhibited HUVEC proliferation, compared with conditioned medium from COS cells transfected with empty plasmid or non-transfected cells. RT-PCR and immunohistochemistry confirmed the expression of K5 in the conjunctiva and cornea. Corneal neovascularization was significantly suppressed by K5 gene transfer in rats′ eyes. Conclusion: In a rat model, K5 gene transfer by subconjunctival injection and electroporation can effectively inhibit corneal neovascularization induced by an alkali burn. Copyright (C) 2003 S. Karger AG, Basel.

语种: 英语
WOS记录号: WOS:000185064700001
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56210
Appears in Collections:北京大学第二临床医学院_眼科_期刊论文

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作者单位: 1.Peking Univ, Gene Ctr, Beijing 100871, Peoples R China
2.Peking Univ, Peoples Hosp, Dept Ophthalmol, Beijing 100044, Peoples R China
3.Peking Univ, Peoples Hosp, Ctr Lab, Beijing 100044, Peoples R China

Recommended Citation:
Yu, WZ,Li, XX,She, HC,et al. Gene transfer of kringle 5 of plasminogen by electroporation inhibits corneal neovascularization[J]. OPHTHALMIC RESEARCH,2003,35(5):239-246.
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