IR@PKUHSC  > 北京大学第一临床医学院  > 皮肤性病科
学科主题临床医学
Multilocus phylogeny and MALDI-TOF analysis of the plant pathogenic species Alternaria dauci and relatives
Brun, Sophie1,2,3; Madrid, Hugo4; Van den Ende, Bert Gerrits4; Andersen, Birgitte5; Marinach-Patrice, Carine2,3; Mazier, Dominique1,2,3; De Hoog, G. Sybren4,6,7,8
关键词Alternaria Maldi-tof Molecular Identification Phylogeny Plant Pathology Pleosporales
刊名FUNGAL BIOLOGY
2013
DOI10.1016/j.funbio.2012.11.003
117期:1页:32-40
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Mycology
研究领域[WOS]Mycology
关键词[WOS]DESORPTION IONIZATION-TIME ; FLIGHT MASS-SPECTROMETRY ; SMALL-SPORED ALTERNARIA ; MORPHOLOGICAL SEGREGATION ; IDENTIFICATION ; THEMES ; MYCOTOXINS ; INFECTORIA ; TAXONOMY ; FUNGI
英文摘要

The genus Alternaria includes numerous phytopathogenic species, many of which are economically relevant. Traditionally, identification has been based on morphology, but is often hampered by the tendency of some strains to become sterile in culture and by the existence of species-complexes of morphologically similar taxa. This study aimed to assess if strains of four closely-related plant pathogens, i.e., accurately Alternaria dauci (ten strains), Alternaria porri (six), Alternaria solani (ten), and Alternaria tomatophila (ten) could be identified using multilocus phylogenetic analysis and Matrix-Assisted Laser Desorption Ionisation Time of Flight (MALDI-TOF) profiling of proteins. Phylogenetic analyses were performed on three loci, i.e., the internal transcribed spacer (ITS) region of rRNA, and the glyceraldehyde-3-phosphate dehydrogenase (gpd) and Alternaria major antigen (Alt a 1) genes. Phylogenetic trees based on ITS sequences did not differentiate strains of A. solani, A. tomatophila, and A. porri, but these three species formed a clade separate from strains of A. dauci. The resolution improved in trees based on gpd and Alt a 1, which distinguished strains of the four species as separate clades. However, none provided significant bootstrap support for all four species, which could only be achieved when results for the three loci were combined. MALDI-TOF-based dendrograms showed three major clusters. The first comprised all A. dauci strains, the second included five strains of A. porri and one of A. solani, and the third included all strains of A. tomatophila, as well as all but one strain of A. solani, and one strain of A. porri. Thus, this study shows the usefulness of MALDI-TOF mass spectrometry as a promising tool for identification of these four species of Alternaria which are closely-related plant pathogens. (C) 2012 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.

语种英语
WOS记录号WOS:000315178200004
引用统计
被引频次:20[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56232
专题北京大学第一临床医学院_皮肤性病科
作者单位1.INSERM, UMRS 945, F-75013 Paris, France
2.Univ Paris 06, Paris, France
3.CBS KNAW Fungal Biodivers Ctr, NL-3508 AD Utrecht, Netherlands
4.Grp Hosp Pitie Salpetriere, AP HP, Serv Parasitol Mycol, F-75013 Paris, France
5.Tech Univ Denmark, Dept Syst Biol, Ctr Microbial Biotechnol CMB, DK-2800 Lyngby, Denmark
6.Univ Amsterdam, Inst Biodivers & Ecosyst Dynam, NL-1090 GE Amsterdam, Netherlands
7.Peking Univ, Hlth Sci Ctr, Res Ctr Med Mycol, Hosp 1, Beijing 100871, Peoples R China
8.Sun Yat Sen Univ, Sun Yat Sen Mem Hosp, Guangzhou 510275, Guangdong, Peoples R China
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GB/T 7714
Brun, Sophie,Madrid, Hugo,Van den Ende, Bert Gerrits,et al. Multilocus phylogeny and MALDI-TOF analysis of the plant pathogenic species Alternaria dauci and relatives[J]. FUNGAL BIOLOGY,2013,117(1):32-40.
APA Brun, Sophie.,Madrid, Hugo.,Van den Ende, Bert Gerrits.,Andersen, Birgitte.,Marinach-Patrice, Carine.,...&De Hoog, G. Sybren.(2013).Multilocus phylogeny and MALDI-TOF analysis of the plant pathogenic species Alternaria dauci and relatives.FUNGAL BIOLOGY,117(1),32-40.
MLA Brun, Sophie,et al."Multilocus phylogeny and MALDI-TOF analysis of the plant pathogenic species Alternaria dauci and relatives".FUNGAL BIOLOGY 117.1(2013):32-40.
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