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Comparison of contents of five ephedrine alkaloids in three official origins of Ephedra Herb in China by high-performance liquid chromatography
Hong, Hao1; Chen, Hu-Biao2; Yang, Dong-Hui1; Shang, Ming-Ying1; Wang, Xuan1; Cai, Shao-Qing1; Mikage, Masayuki3
关键词Ephedra Herb Ephedra Alkaloids Simultaneous Determination Mahuang Species Identification
刊名JOURNAL OF NATURAL MEDICINES
2011-07-01
DOI10.1007/s11418-011-0528-8
65期:3-4页:623-628
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Medicinal ; Pharmacology & Pharmacy
研究领域[WOS]Pharmacology & Pharmacy
英文摘要

In the present paper, a fast and economical HPLC method [on a Phenomenex Polar-RP column with a solution of (phosphoric acid:triethylamine:dibutylamine:water = 0.40:0.1:0.2:499.3) and methanol] is developed, and applied for the determination of norephedrine, norpseudoephedrine, ephedrine (E), pseudoephedrine (PE) and methylephedrine (ME) in 64 samples of three species from main habitats in China. Quantitation data showed that total alkaloid content in Ephedra equisetina Bge. (2.708 +/- A 0.642%) is higher than that in E. sinica Stapf. (1.365 +/- A 0.624%) and E. intermedia Schrenk et C. A. Mey. (1.537 +/- A 0.746%), but the range of total alkaloid content in each species is so wide that the ranges of the three species greatly overlap. The contents of E, PE and ME are different among the three species. The ratio E/total alkaloid content and ratio E/PE as well as E and ME contents can be used for identification of E. sinica, E. intermedia and E. equisetina from one another.

语种英语
WOS记录号WOS:000291746900030
项目编号30425018
资助机构National Outstanding Youth Funds of China
引用统计
被引频次:11[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56246
专题北京大学药学院
北京大学药学院_化学生物学系
北京大学药学院_天然药物学系
作者单位1.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
2.Hong Kong Baptist Univ, Sch Chinese Med, Hong Kong, Hong Kong, Peoples R China
3.Kanazawa Univ, Grad Sch Nat Sci & Technol, Kanazawa, Ishikawa 9201192, Japan
推荐引用方式
GB/T 7714
Hong, Hao,Chen, Hu-Biao,Yang, Dong-Hui,et al. Comparison of contents of five ephedrine alkaloids in three official origins of Ephedra Herb in China by high-performance liquid chromatography[J]. JOURNAL OF NATURAL MEDICINES,2011,65(3-4):623-628.
APA Hong, Hao.,Chen, Hu-Biao.,Yang, Dong-Hui.,Shang, Ming-Ying.,Wang, Xuan.,...&Mikage, Masayuki.(2011).Comparison of contents of five ephedrine alkaloids in three official origins of Ephedra Herb in China by high-performance liquid chromatography.JOURNAL OF NATURAL MEDICINES,65(3-4),623-628.
MLA Hong, Hao,et al."Comparison of contents of five ephedrine alkaloids in three official origins of Ephedra Herb in China by high-performance liquid chromatography".JOURNAL OF NATURAL MEDICINES 65.3-4(2011):623-628.
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