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学科主题: 临床医学
题名:
Nanosecond Pulsed Electric Fields (nsPEFs) Regulate Phenotypes of Chondrocytes through Wnt/beta-catenin Signaling Pathway
作者: Zhang, Kun1; Guo, Jinsong2; Ge, Zigang1,3,4,5; Zhang, Jue2,6
刊名: SCIENTIFIC REPORTS
发表日期: 2014-07-25
DOI: 10.1038/srep05836
卷: 4
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: BETA-CATENIN ; ARTICULAR-CARTILAGE ; MEMBRANE PERMEABILIZATION ; COLLAGEN GENE ; HUMAN-CELLS ; IN-VITRO ; EXPRESSION ; STIMULATION ; ACTIVATION ; APOPTOSIS
英文摘要:

Nanosecond pulsed electric fields (nsPEFs) characterized by high voltage, low energy and non-thermal effects, have been broadly investigated as a potential tumor therapy; however, little is known about their effects on somatic cells. In this current study, we evaluated effects of nsPEFs on the phenotype of chondrocytes (morphology, glycosaminoglycan (GAG) content, proliferation and gene expression) and explored the mechanisms involved. Our results demonstrated that exposing chondrocytes to nsPEFs led to enhanced proliferation and dedifferentiation, evidenced by the upregulated gene expression of collagen type I (COL I) and downregulated gene expression of Sox9, collagen type II (COL II) and aggrecan (AGG) with activation of the wnt/beta-catenin signaling pathway. Inhibition of the wnt/beta-catenin pathway partially blocked these effects. Thus we concluded that nsPEFs induce dedifferentiation of chondrocytes partially through transient activation of the wnt/beta-catenin signaling pathway.

语种: 英语
所属项目编号: 2012CB619100 ; 81271722
项目资助者: National Basic Research Program of China (973 Program) ; National Natural Science Foundation of China grant
WOS记录号: WOS:000339421300017
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56248
Appears in Collections:北京大学第二临床医学院_期刊论文

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作者单位: 1.Peking Univ, Dept Biomed Engn, Coll Engn, Beijing 100871, Peoples R China
2.Peking Univ, Inst Biomech & Biomed Engn, Coll Engn, Beijing 100871, Peoples R China
3.Peking Univ, Acad Adv Interdisciplinary Studies, Ctr Biomed Mat & Tissue Engn, Beijing 100871, Peoples R China
4.Peking Univ, Peoples Hosp, Arthritis Clin, Beijing 100871, Peoples R China
5.Peking Univ, Peoples Hosp, Res Ctr, Beijing 100871, Peoples R China
6.Peking Univ, Acad Adv Interdisciplinary Studies, Ctr BioMed X Res, Beijing 100871, Peoples R China

Recommended Citation:
Zhang, Kun,Guo, Jinsong,Ge, Zigang,et al. Nanosecond Pulsed Electric Fields (nsPEFs) Regulate Phenotypes of Chondrocytes through Wnt/beta-catenin Signaling Pathway[J]. SCIENTIFIC REPORTS,2014,4.
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