IR@PKUHSC  > 北京大学第二临床医学院
学科主题临床医学
Nanosecond Pulsed Electric Fields (nsPEFs) Regulate Phenotypes of Chondrocytes through Wnt/beta-catenin Signaling Pathway
Zhang, Kun1; Guo, Jinsong2; Ge, Zigang1,3,4,5; Zhang, Jue2,6
刊名SCIENTIFIC REPORTS
2014-07-25
DOI10.1038/srep05836
4
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
资助者National Basic Research Program of China (973 Program) ; National Natural Science Foundation of China grant ; National Basic Research Program of China (973 Program) ; National Natural Science Foundation of China grant
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]BETA-CATENIN ; ARTICULAR-CARTILAGE ; MEMBRANE PERMEABILIZATION ; COLLAGEN GENE ; HUMAN-CELLS ; IN-VITRO ; EXPRESSION ; STIMULATION ; ACTIVATION ; APOPTOSIS
英文摘要

Nanosecond pulsed electric fields (nsPEFs) characterized by high voltage, low energy and non-thermal effects, have been broadly investigated as a potential tumor therapy; however, little is known about their effects on somatic cells. In this current study, we evaluated effects of nsPEFs on the phenotype of chondrocytes (morphology, glycosaminoglycan (GAG) content, proliferation and gene expression) and explored the mechanisms involved. Our results demonstrated that exposing chondrocytes to nsPEFs led to enhanced proliferation and dedifferentiation, evidenced by the upregulated gene expression of collagen type I (COL I) and downregulated gene expression of Sox9, collagen type II (COL II) and aggrecan (AGG) with activation of the wnt/beta-catenin signaling pathway. Inhibition of the wnt/beta-catenin pathway partially blocked these effects. Thus we concluded that nsPEFs induce dedifferentiation of chondrocytes partially through transient activation of the wnt/beta-catenin signaling pathway.

语种英语
所属项目编号2012CB619100 ; 81271722
资助者National Basic Research Program of China (973 Program) ; National Natural Science Foundation of China grant ; National Basic Research Program of China (973 Program) ; National Natural Science Foundation of China grant
WOS记录号WOS:000339421300017
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56248
Collection北京大学第二临床医学院
作者单位1.Peking Univ, Dept Biomed Engn, Coll Engn, Beijing 100871, Peoples R China
2.Peking Univ, Inst Biomech & Biomed Engn, Coll Engn, Beijing 100871, Peoples R China
3.Peking Univ, Acad Adv Interdisciplinary Studies, Ctr Biomed Mat & Tissue Engn, Beijing 100871, Peoples R China
4.Peking Univ, Peoples Hosp, Arthritis Clin, Beijing 100871, Peoples R China
5.Peking Univ, Peoples Hosp, Res Ctr, Beijing 100871, Peoples R China
6.Peking Univ, Acad Adv Interdisciplinary Studies, Ctr BioMed X Res, Beijing 100871, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Kun,Guo, Jinsong,Ge, Zigang,et al. Nanosecond Pulsed Electric Fields (nsPEFs) Regulate Phenotypes of Chondrocytes through Wnt/beta-catenin Signaling Pathway[J]. SCIENTIFIC REPORTS,2014,4.
APA Zhang, Kun,Guo, Jinsong,Ge, Zigang,&Zhang, Jue.(2014).Nanosecond Pulsed Electric Fields (nsPEFs) Regulate Phenotypes of Chondrocytes through Wnt/beta-catenin Signaling Pathway.SCIENTIFIC REPORTS,4.
MLA Zhang, Kun,et al."Nanosecond Pulsed Electric Fields (nsPEFs) Regulate Phenotypes of Chondrocytes through Wnt/beta-catenin Signaling Pathway".SCIENTIFIC REPORTS 4(2014).
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