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学科主题: 临床医学
题名:
Quantitation of rare circulating tumor cells by folate receptor alpha ligand-targeted PCR in bladder transitional cell carcinoma and its potential diagnostic significance
作者: Qi, Fuming1,2,3; Liu, Yuchen1,2,4; Zhao, Rongchang4,7; Zou, Xiangjun1,4; Zhang, Lei4,6; Li, Jiaqiang2,4; Wang, Yongqiang4; Li, Feiyang4; Zou, Xiaowen4; Xia, Ye2,4; Wang, Xuliang2,4; Xing, Li4,6; Li, Cailing2; Lu, Jingxiao8; Tang, Junlong8; Zhou, Fangjian5; Liu, Chunxiao6; Gui, Yaoting2; Cai, Zhiming1,4; Sun, Xiaojuan8
关键词: Bladder transitional cell carcinoma ; Tumor specific ligand-PCR ; Circulatingtumor cell ; Folate receptor alpha
刊名: TUMOR BIOLOGY
发表日期: 2014-07-01
DOI: 10.1007/s13277-014-1894-0
卷: 35, 期:7, 页:7217-7223
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: CANCER ; CYTOLOGY ; OLIGOCLONALITY ; SURVEILLANCE ; SPECIFICITY ; RECURRENCE ; PREDICTION ; ACCURACY ; MARKERS
英文摘要:

Numerous attempts for detection of circulating tumor cells (CTC) have been made to develop reliable assays for early diagnosis of cancers. In this study, we validated the application of folate receptor alpha (FR alpha) as the tumor marker to detect CTC through tumor-specific ligand PCR (LT-PCR) and assessed its utility for diagnosis of bladder transitional cell carcinoma (TCC). Immunohistochemistry for FR alpha was performed on ten bladder TCC tissues. Enzyme-linked immunosorbent assay (ELISA) for FR alpha was performed on both urine and serum specimens from bladder TCC patients (n = 64 and n = 20, respectively) and healthy volunteers (n = 20 and n = 23, respectively). Western blot analysis and qRT-PCR were performed to confirm the expression of FR alpha in bladder TCC cells. CTC values in 3-mL peripheral blood were measured in 57 bladder TCC patients, 48 healthy volunteers, and 15 subjects with benign urologic pathologies by the folate receptor alpha ligand-targeted PCR. We found that FR alpha protein was overexpressed in both bladder TCC cells and tissues. The levels of FR alpha mRNA were also much higher in bladder cancer cell lines 5637 and SW780 than those of leukocyte. Values of FR alpha were higher in both serum and urine specimens of bladder TCC patients than those of control. CTC values were also higher in 3-mL peripheral blood of bladder TCC patients than those of control (median 26.5 Cu/3 mL vs 14.0 Cu/3 mL). Area under the receiver operating characteristic (ROC) curve for bladder TCC detection was 0.819, 95 % CI (0.738-0.883). At the cutoff value of 15.43 Cu/3 mL, the sensitivity and the specificity for detecting bladder cancer are 82.14 and 61.9 %, respectively. We concluded that quantitation of CTCs through FR alpha ligand-PCR could be a promising method for noninvasive diagnosis of bladder TCC.

语种: 英语
所属项目编号: NSFC 81372507 ; 81101584 ; 338 2014CB745200 ; KQC201106020032A ; JCYJ20120614455816670 ; 2014DFA31050
项目资助者: National Nature Science Foundation of China ; National Basic Research Program of China (973 Program) ; Shenzhen Peacock Foundation ; Shenzhen Basic Research Key Project ; International S&amp ; T Cooperation Program of China
WOS记录号: WOS:000339736300129
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56262
Appears in Collections:北京大学深圳医院_期刊论文

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作者单位: 1.Shantou Univ, Coll Med, Shantou, Peoples R China
2.Shenzhen Univ, Affiliated Hosp 1, Shenzhen Peoples Hosp 2, Dept Urol Surg, Shenzhen, Peoples R China
3.Peking Univ, Shenzhen PKU HKUST Med Ctr, Guangdong & Shenzhen Key Lab Male Reprod Med & Ge, Inst Urol,Shenzhen Hosp, Shenzhen, Peoples R China
4.Shenzhen Univ, Affiliated Hosp 1, Shenzhen Peoples Hosp 2, Shenzhen Key Lab Genitourinary Tumor, Shenzhen 518035, Peoples R China
5.Sun Yat Sen Univ, Ctr Canc, Dept Urol, Guangzhou 510275, Guangdong, Peoples R China
6.Southern Med Univ, Zhujiang Hosp, Dept Urol, Guangzhou, Guangdong, Peoples R China
7.GenoSaber Biotech Co Ltd, Dept Res & Dev, Shanghai, Peoples R China
8.Shenzhen Univ, Affiliated Hosp 1, Shenzhen Tumor Immunogene Therapy Clinin Applicat, Biobank,Shenzhen Peoples Hosp 2, Shenzhen 518035, Peoples R China

Recommended Citation:
Qi, Fuming,Liu, Yuchen,Zhao, Rongchang,et al. Quantitation of rare circulating tumor cells by folate receptor alpha ligand-targeted PCR in bladder transitional cell carcinoma and its potential diagnostic significance[J]. TUMOR BIOLOGY,2014,35(7):7217-7223.
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