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Quantitation of rare circulating tumor cells by folate receptor alpha ligand-targeted PCR in bladder transitional cell carcinoma and its potential diagnostic significance
Qi, Fuming1,2,3; Liu, Yuchen1,2,4; Zhao, Rongchang4,7; Zou, Xiangjun1,4; Zhang, Lei4,6; Li, Jiaqiang2,4; Wang, Yongqiang4; Li, Feiyang4; Zou, Xiaowen4; Xia, Ye2,4; Wang, Xuliang2,4; Xing, Li4,6; Li, Cailing2; Lu, Jingxiao8; Tang, Junlong8; Zhou, Fangjian5; Liu, Chunxiao6; Gui, Yaoting2; Cai, Zhiming1,4; Sun, Xiaojuan8
关键词Bladder Transitional Cell Carcinoma Tumor Specific Ligand-pcr Circulatingtumor Cell Folate Receptor Alpha
刊名TUMOR BIOLOGY
2014-07-01
DOI10.1007/s13277-014-1894-0
35期:7页:7217-7223
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
研究领域[WOS]Oncology
关键词[WOS]CANCER ; CYTOLOGY ; OLIGOCLONALITY ; SURVEILLANCE ; SPECIFICITY ; RECURRENCE ; PREDICTION ; ACCURACY ; MARKERS
英文摘要

Numerous attempts for detection of circulating tumor cells (CTC) have been made to develop reliable assays for early diagnosis of cancers. In this study, we validated the application of folate receptor alpha (FR alpha) as the tumor marker to detect CTC through tumor-specific ligand PCR (LT-PCR) and assessed its utility for diagnosis of bladder transitional cell carcinoma (TCC). Immunohistochemistry for FR alpha was performed on ten bladder TCC tissues. Enzyme-linked immunosorbent assay (ELISA) for FR alpha was performed on both urine and serum specimens from bladder TCC patients (n = 64 and n = 20, respectively) and healthy volunteers (n = 20 and n = 23, respectively). Western blot analysis and qRT-PCR were performed to confirm the expression of FR alpha in bladder TCC cells. CTC values in 3-mL peripheral blood were measured in 57 bladder TCC patients, 48 healthy volunteers, and 15 subjects with benign urologic pathologies by the folate receptor alpha ligand-targeted PCR. We found that FR alpha protein was overexpressed in both bladder TCC cells and tissues. The levels of FR alpha mRNA were also much higher in bladder cancer cell lines 5637 and SW780 than those of leukocyte. Values of FR alpha were higher in both serum and urine specimens of bladder TCC patients than those of control. CTC values were also higher in 3-mL peripheral blood of bladder TCC patients than those of control (median 26.5 Cu/3 mL vs 14.0 Cu/3 mL). Area under the receiver operating characteristic (ROC) curve for bladder TCC detection was 0.819, 95 % CI (0.738-0.883). At the cutoff value of 15.43 Cu/3 mL, the sensitivity and the specificity for detecting bladder cancer are 82.14 and 61.9 %, respectively. We concluded that quantitation of CTCs through FR alpha ligand-PCR could be a promising method for noninvasive diagnosis of bladder TCC.

语种英语
WOS记录号WOS:000339736300129
项目编号NSFC 81372507 ; 81101584 ; 338 2014CB745200 ; KQC201106020032A ; JCYJ20120614455816670 ; 2014DFA31050
资助机构National Nature Science Foundation of China ; National Basic Research Program of China (973 Program) ; Shenzhen Peacock Foundation ; Shenzhen Basic Research Key Project ; International S&amp ; T Cooperation Program of China
引用统计
被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56262
专题北京大学深圳医院
作者单位1.Shantou Univ, Coll Med, Shantou, Peoples R China
2.Shenzhen Univ, Affiliated Hosp 1, Shenzhen Peoples Hosp 2, Dept Urol Surg, Shenzhen, Peoples R China
3.Peking Univ, Shenzhen PKU HKUST Med Ctr, Guangdong & Shenzhen Key Lab Male Reprod Med & Ge, Inst Urol,Shenzhen Hosp, Shenzhen, Peoples R China
4.Shenzhen Univ, Affiliated Hosp 1, Shenzhen Peoples Hosp 2, Shenzhen Key Lab Genitourinary Tumor, Shenzhen 518035, Peoples R China
5.Sun Yat Sen Univ, Ctr Canc, Dept Urol, Guangzhou 510275, Guangdong, Peoples R China
6.Southern Med Univ, Zhujiang Hosp, Dept Urol, Guangzhou, Guangdong, Peoples R China
7.GenoSaber Biotech Co Ltd, Dept Res & Dev, Shanghai, Peoples R China
8.Shenzhen Univ, Affiliated Hosp 1, Shenzhen Tumor Immunogene Therapy Clinin Applicat, Biobank,Shenzhen Peoples Hosp 2, Shenzhen 518035, Peoples R China
推荐引用方式
GB/T 7714
Qi, Fuming,Liu, Yuchen,Zhao, Rongchang,et al. Quantitation of rare circulating tumor cells by folate receptor alpha ligand-targeted PCR in bladder transitional cell carcinoma and its potential diagnostic significance[J]. TUMOR BIOLOGY,2014,35(7):7217-7223.
APA Qi, Fuming.,Liu, Yuchen.,Zhao, Rongchang.,Zou, Xiangjun.,Zhang, Lei.,...&Sun, Xiaojuan.(2014).Quantitation of rare circulating tumor cells by folate receptor alpha ligand-targeted PCR in bladder transitional cell carcinoma and its potential diagnostic significance.TUMOR BIOLOGY,35(7),7217-7223.
MLA Qi, Fuming,et al."Quantitation of rare circulating tumor cells by folate receptor alpha ligand-targeted PCR in bladder transitional cell carcinoma and its potential diagnostic significance".TUMOR BIOLOGY 35.7(2014):7217-7223.
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