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学科主题: 药学
题名:
Homo log-focused profiling of ginsenosides based on the integration of step-wise formate anion-to-deprotonated ion transition screening and scheduled multiple reaction monitoring
作者: Song, Yuelin1; Zhang, Na1,2,3; Shi, Shepo1; Li, Jun1; Zhao, Yunfang1; Zhang, Qian1; Jiang, Yong4; Tu, Pengfei1
关键词: Homolog-focused profiling ; Ginsenoside ; Step-wise multiple reaction monitoring ; Scheduled multiple reaction monitoring ; Formate anion-to-deprotonated ion pairing
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2015-08-07
DOI: 10.1016/j.chroma.2015.06.007
卷: 1406, 页:136-144
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: TANDEM MASS-SPECTROMETRY ; LIQUID-CHROMATOGRAPHY ; PANAX-NOTOGINSENG ; ENANTIOSPECIFIC DETERMINATION ; COLUMN CHROMATOGRAPHY ; METABOLOMICS ; SAPONINS ; GINSENG ; QUANTIFICATION ; PESTICIDES
英文摘要:

Homolog-focused profiling is a favored option to bridge targeted metabolomics toward non-targeted metabolomics. In current study, an attempt was made for the large-scale ginsenoside-specific analysis in ginseng (G) and American ginseng (AG). When formic acid (0.1%, v/v) was introduced as the mobile phase additive, formate anion-to-deprotonated ion transitions ([M+HCOO](-) > [M-H](-)) with an optimal collision energy (-32 eV) could result in satisfactory responses for ginsenosides. Therefore, a step-wise multiple reaction monitoring (MRM)-based method employing [M+HCOO](-) > [M-H](-) ion pairs was constructed to screen ginsenosides among 501-1250 u (for Q1) with a step-size of 2 u, and MRM also served as a survey experiment to trigger enhanced product ion scans for MS2 spectrum acquisition on a hybrid triple quadrupole-linear ion trap mass spectrometer; then, the identification of those observed ginsenosides was achieved on the basis of the well-defined mass cracking patterns for ginsenosides; afterwards, scheduled MRM was introduced for large-scale relatively quantitative analysis of all detected ginsenosides. Finally, comparative metabolomics were performed to differentiate G, AG, and their processed products. Method validation was carried out using thirteen authentic compounds. A total of 221 ginsenosides, among which 185 ones were annotated, were observed and relatively quantitated. All crude materials were obviously classified into groups I-III. Above all, the MRM-based homolog-focused profiling of ginsenosides could be used as a reliable tool to gain an in-depth view for ginsenoside-enriched herbal products. (C) 2015 Elsevier B.V. All rights reserved.

语种: 英语
所属项目编号: 81403073 ; 81222051 ; 2013ZX09201018 ; 2012ZX09103201-036 ; 2012ZX09301002-002-002
项目资助者: National Science Fund of China ; National Key Technology R&amp ; D Program "New Drug Innovation" of China
WOS记录号: WOS:000358094200017
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56268
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Baotou Med Coll, Baotou 014060, Peoples R China
2.Being Univ Chinese Med, Modern Res Ctr Tradit Chinese Med, Beijing 100029, Peoples R China
3.Beijing Univ Chinese Med, Sch Chinese Mat Medica, Beijing 100102, Peoples R China
4.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China

Recommended Citation:
Song, Yuelin,Zhang, Na,Shi, Shepo,et al. Homo log-focused profiling of ginsenosides based on the integration of step-wise formate anion-to-deprotonated ion transition screening and scheduled multiple reaction monitoring[J]. JOURNAL OF CHROMATOGRAPHY A,2015,1406:136-144.
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