学科主题基础医学
Rearrangement and expression of the immunoglobulin mu-chain gene in human myeloid cells
Huang, Jing1,2; Sun, Xiaoping3; Gong, Xiaoting1,2; He, Zhiqiao4; Chen, Lei5; Qiu, Xiaoyan1,2,4; Yin, C. Cameron4
关键词acute myeloid leukemia IgM V(H mu)DJ(H mu) gene rearrangements
刊名CELLULAR & MOLECULAR IMMUNOLOGY
2014
DOI10.1038/cmi.2013.45
11期:1页:94-104
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Immunology
研究领域[WOS]Immunology
关键词[WOS]EPITHELIAL CANCER-CELLS ; KAPPA LIGHT-CHAIN ; HEAVY-CHAIN ; TUMOR-CELLS ; REPERTOIRE ; LEUKEMIA ; LINES ; LYMPHOMA ; SEQUENCE ; REGION
英文摘要

Immunoglobulin (Ig), a characteristic marker of B cells, has been reported to be expressed in epithelial cells, with a suggested role in their growth and survival. We have previously reported that IgG heavy chain is expressed in acute myeloid leukemia (AML), but not in the monocytes or neutrophils from patients with non-hematopoietic neoplasms or healthy controls. In the present study, we assessed IgM heavy chain expression and repertoire in human myeloid cells. We detected V(H mu)DJ(H mu) rearrangement and expression in 7/7 AML cell lines, 7/14 primary myeloblasts from AML patients, and interestingly, 8/20 monocytes and 3/20 neutrophils from patients with non-hematopoietic neoplasms and healthy individuals. We also found evidence of somatic hypermutation of the variable (V) gene segments in AML-derived IgM gene rearrangements but not in IgM from monocytes or neutrophils from patients with non-hematopoietic neoplasms and healthy individuals. Furthermore, IgM V(H mu)DJ(H mu) gene rearrangements in AML cell lines, primary myeloblasts, and monocytes and neutrophils from patients with non-hematopoietic neoplasms showed a restricted V usage and repertoire, whereas the V(H mu)DJ(H mu) gene rearrangements in monocytes and neutrophils from healthy individuals displayed more diversity. Anti-human IgM inhibited cell proliferation, but did not induce apoptosis in AML cell lines. Our findings suggest that AML-derived IgM might be a novel AML-related molecule that is involved in leukemogenesis and AML progression and might serve as a useful molecular marker for designing targeted therapy and monitoring minimal residual disease.

语种英语
WOS记录号WOS:000329313900013
项目编号18079170 ; 81320108020
资助机构University of Texas MD Anderson Cancer Center ; National Natural Sciences Foundation of China
引用统计
被引频次:14[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56272
专题北京大学基础医学院_北京大学人类疾病基因研究中心
北京大学基础医学院
作者单位1.Peking Univ, Ctr Human Dis Genom, Beijing 100871, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Minist Hlth, Sch Basic Med Sci,Dept Immunol,Key Lab Immunol, Beijing 100871, Peoples R China
3.Univ Texas MD Anderson Canc Ctr, Dept Lab Med, Houston, TX 77030 USA
4.Univ Texas MD Anderson Canc Ctr, Dept Hematopathol, Houston, TX 77030 USA
5.Univ Texas Houston, Sch Med, Dept Pathol, Houston, TX 77030 USA
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Huang, Jing,Sun, Xiaoping,Gong, Xiaoting,et al. Rearrangement and expression of the immunoglobulin mu-chain gene in human myeloid cells[J]. CELLULAR & MOLECULAR IMMUNOLOGY,2014,11(1):94-104.
APA Huang, Jing.,Sun, Xiaoping.,Gong, Xiaoting.,He, Zhiqiao.,Chen, Lei.,...&Yin, C. Cameron.(2014).Rearrangement and expression of the immunoglobulin mu-chain gene in human myeloid cells.CELLULAR & MOLECULAR IMMUNOLOGY,11(1),94-104.
MLA Huang, Jing,et al."Rearrangement and expression of the immunoglobulin mu-chain gene in human myeloid cells".CELLULAR & MOLECULAR IMMUNOLOGY 11.1(2014):94-104.
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