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Identification of Lactobacillus from the Saliva of Adult Patients with Caries Using Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry
Zhang, Yifei1; Liu, Yingyi2; Ma, Qingwei3; Song, Yeqing1; Zhang, Qian1; Wang, Xiaoyan2; Chen, Feng1
刊名PLOS ONE
2014-08-28
DOI10.1371/journal.pone.0106185
9期:8
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]MALDI-TOF MS ; DENTAL-CARIES ; RAPID IDENTIFICATION ; MUTANS STREPTOCOCCI ; ACTIVITY TESTS ; CHILDREN ; MICROORGANISMS ; BACTERIAL ; ADOLESCENTS ; PREVALENCE
英文摘要

Matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry (MS) has been presented as a superior method for the detection of microorganisms in body fluid samples (e.g., blood, saliva, pus, etc.) However, the performance of MALDI-TOF MS in routine identification of caries-related Lactobacillus isolates from saliva of adult patients with caries has not been determined. In the present study, we introduced a new MALDI-TOF MS system for identification of lactobacilli. Saliva samples were collected from 120 subjects with caries. Bacteria were isolated and cultured, and each isolate was identified by both 16S rRNA sequencing and MALDI-TOF MS. The identification results obtained by MALDI-TOF MS were concordant at the genus level with those of conventional 16S rRNA-based sequencing for 88.6% of lactobacilli (62/70) and 95.5% of non-lactobacilli (21/22). Up to 96 results could be obtained in parallel on a single MALDI target, suggesting that this is a reliable high-throughput approach for routine identification of lactobacilli. However, additional reference strains are necessary to increase the sensitivity and specificity of species-level identification.

语种英语
WOS记录号WOS:000341303700081
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56282
专题北京大学口腔医学院
北京大学口腔医学院_牙体牙髓科
北京大学口腔医学院_中心实验室
作者单位1.Peking Univ, Sch & Hosp Stomatol, Cent Lab, Beijing 100871, Peoples R China
2.Peking Univ, Sch & Hosp Stomatol, Dept Cariol & Endodontol, Beijing 100871, Peoples R China
3.Peking Univ, Acad Adv Interdisciplinary Studies, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Zhang, Yifei,Liu, Yingyi,Ma, Qingwei,et al. Identification of Lactobacillus from the Saliva of Adult Patients with Caries Using Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry[J]. PLOS ONE,2014,9(8).
APA Zhang, Yifei.,Liu, Yingyi.,Ma, Qingwei.,Song, Yeqing.,Zhang, Qian.,...&Chen, Feng.(2014).Identification of Lactobacillus from the Saliva of Adult Patients with Caries Using Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry.PLOS ONE,9(8).
MLA Zhang, Yifei,et al."Identification of Lactobacillus from the Saliva of Adult Patients with Caries Using Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry".PLOS ONE 9.8(2014).
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