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学科主题: 口腔医学
题名:
Identification of Lactobacillus from the Saliva of Adult Patients with Caries Using Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry
作者: Zhang, Yifei1; Liu, Yingyi2; Ma, Qingwei3; Song, Yeqing1; Zhang, Qian1; Wang, Xiaoyan2; Chen, Feng1
刊名: PLOS ONE
发表日期: 2014-08-28
DOI: 10.1371/journal.pone.0106185
卷: 9, 期:8
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: MALDI-TOF MS ; DENTAL-CARIES ; RAPID IDENTIFICATION ; MUTANS STREPTOCOCCI ; ACTIVITY TESTS ; CHILDREN ; MICROORGANISMS ; BACTERIAL ; ADOLESCENTS ; PREVALENCE
英文摘要:

Matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry (MS) has been presented as a superior method for the detection of microorganisms in body fluid samples (e.g., blood, saliva, pus, etc.) However, the performance of MALDI-TOF MS in routine identification of caries-related Lactobacillus isolates from saliva of adult patients with caries has not been determined. In the present study, we introduced a new MALDI-TOF MS system for identification of lactobacilli. Saliva samples were collected from 120 subjects with caries. Bacteria were isolated and cultured, and each isolate was identified by both 16S rRNA sequencing and MALDI-TOF MS. The identification results obtained by MALDI-TOF MS were concordant at the genus level with those of conventional 16S rRNA-based sequencing for 88.6% of lactobacilli (62/70) and 95.5% of non-lactobacilli (21/22). Up to 96 results could be obtained in parallel on a single MALDI target, suggesting that this is a reliable high-throughput approach for routine identification of lactobacilli. However, additional reference strains are necessary to increase the sensitivity and specificity of species-level identification.

语种: 英语
所属项目编号: 81300880 ; 81200762 ; PKUSS20110301
项目资助者: National Natural Science Foundation of China ; Peking University School of Stomatology
WOS记录号: WOS:000341303700081
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56282
Appears in Collections:北京大学口腔医学院_期刊论文

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作者单位: 1.Peking Univ, Sch & Hosp Stomatol, Cent Lab, Beijing 100871, Peoples R China
2.Peking Univ, Sch & Hosp Stomatol, Dept Cariol & Endodontol, Beijing 100871, Peoples R China
3.Peking Univ, Acad Adv Interdisciplinary Studies, Beijing 100871, Peoples R China

Recommended Citation:
Zhang, Yifei,Liu, Yingyi,Ma, Qingwei,et al. Identification of Lactobacillus from the Saliva of Adult Patients with Caries Using Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry[J]. PLOS ONE,2014,9(8).
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