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学科主题: 临床医学
题名:
Optimal timing of oocyte maturation and its relationship with the spindle assembly and developmental competence of in vitro matured human oocytes
作者: Yu, Yang1,2,3; Yan, Jie1,3; Liu, Zi C.2,3,4; Yan, Li Y.1,3; Li, Min1,3; Zhou, Qi2; Qiao, Jie1,3
关键词: Culture duration ; embryo quality ; human in vitro matured oocytes ; parthenogenetic activation ; spindle assembly
刊名: FERTILITY AND STERILITY
发表日期: 2011-07-01
DOI: 10.1016/j.fertnstert.2011.04.077
卷: 96, 期:1, 页:73-U481
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Obstetrics & Gynecology ; Reproductive Biology
研究领域[WOS]: Obstetrics & Gynecology ; Reproductive Biology
关键词[WOS]: HUMAN PARTHENOGENETIC BLASTOCYSTS ; INTRACYTOPLASMIC SPERM INJECTION ; STEM-CELL LINES ; POLAR BODY ; EMBRYO QUALITY ; FERTILIZATION ; ACTIVATION ; PREGNANCY ; ORGANIZATION ; PROGRESSION
英文摘要:

Objective: To develop and test a simple, feasible approach to improve spindle assembly and developmental competence of human in vitro matured oocytes by parthenogenetic activation in maturation medium.

Design: Prospective, randomized study.

Setting: University research laboratory-based assisted reproductive technology laboratory.

Patient(s): Four hundred thirty-two patients with male factor infertility undergoing intracytoplasmic sperm injection (ICSI) cycles.

Intervention(s): In vivo failed-to-mature oocytes from the ICSI cycles were divided into four groups according to differing duration after the extrusion of first polar body (0-1, 2-3, 4-5, and 8-9 hours). Oocytes spindles in each group were immunostained for alpha-tubulin and chromosomes, and observed by confocal microscopy.

Main Outcome Measure(s): Rate from pronuclear stage to blastocyst, embryo grading at the eight-cell and blastocyst stages, and spindle assembly.

Result(s): There was a statistically significantly higher rate of development at the eight-cell and blastocyst stages in the 2- to 3-hour and 4- to 5-hour groups. The grading results at the eight-cell and blastocyst stages also showed that the proportion of embryos of high quality was similar among the 2- to 3-hour and 4- to 5-hour groups, and in these groups it was statistically significantly higher than the 0- to 1-hour and 8- to 9-hour groups. The results of immunofluorescence demonstrated that there was a statistically significantly higher rate of normal spindle assembly in the 2- to 3-hour and 4- to 5-hour groups.

Conclusion(s): Optimal timing of maturation benefits the development of competence of in vitro matured oocytes by promoting normal spindle assembly. (Fertil Steril (R) 2011;96:73-8. (C)2011 by American Society for Reproductive Medicine.)

语种: 英语
所属项目编号: 2006AA02A101 ; 2011CB944504
项目资助者: National High Technology Research and Development Program of China ; Ministry of Science and Technology of China
WOS记录号: WOS:000292283000035
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56285
Appears in Collections:北京大学第三临床医学院_期刊论文

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作者单位: 1.Chinese Acad Sci, Peking Univ Third Hosp, Dept Obstet & Gynecol, Beijing 100191, Peoples R China
2.Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing 100191, Peoples R China
3.Chinese Acad Sci, Minist Educ, Key Lab Assisted Reprod, Beijing 100191, Peoples R China
4.Chinese Acad Sci, Grad Sch, Beijing 100191, Peoples R China

Recommended Citation:
Yu, Yang,Yan, Jie,Liu, Zi C.,et al. Optimal timing of oocyte maturation and its relationship with the spindle assembly and developmental competence of in vitro matured human oocytes[J]. FERTILITY AND STERILITY,2011,96(1):73-U481.
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