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学科主题临床医学
Optimal timing of oocyte maturation and its relationship with the spindle assembly and developmental competence of in vitro matured human oocytes
Yu, Yang1,2,3; Yan, Jie1,3; Liu, Zi C.2,3,4; Yan, Li Y.1,3; Li, Min1,3; Zhou, Qi2; Qiao, Jie1,3
关键词Culture Duration Embryo Quality Human In Vitro Matured Oocytes Parthenogenetic Activation Spindle Assembly
刊名FERTILITY AND STERILITY
2011-07-01
DOI10.1016/j.fertnstert.2011.04.077
96期:1页:73-U481
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Obstetrics & Gynecology ; Reproductive Biology
研究领域[WOS]Obstetrics & Gynecology ; Reproductive Biology
关键词[WOS]HUMAN PARTHENOGENETIC BLASTOCYSTS ; INTRACYTOPLASMIC SPERM INJECTION ; STEM-CELL LINES ; POLAR BODY ; EMBRYO QUALITY ; FERTILIZATION ; ACTIVATION ; PREGNANCY ; ORGANIZATION ; PROGRESSION
英文摘要

Objective: To develop and test a simple, feasible approach to improve spindle assembly and developmental competence of human in vitro matured oocytes by parthenogenetic activation in maturation medium.

Design: Prospective, randomized study.

Setting: University research laboratory-based assisted reproductive technology laboratory.

Patient(s): Four hundred thirty-two patients with male factor infertility undergoing intracytoplasmic sperm injection (ICSI) cycles.

Intervention(s): In vivo failed-to-mature oocytes from the ICSI cycles were divided into four groups according to differing duration after the extrusion of first polar body (0-1, 2-3, 4-5, and 8-9 hours). Oocytes spindles in each group were immunostained for alpha-tubulin and chromosomes, and observed by confocal microscopy.

Main Outcome Measure(s): Rate from pronuclear stage to blastocyst, embryo grading at the eight-cell and blastocyst stages, and spindle assembly.

Result(s): There was a statistically significantly higher rate of development at the eight-cell and blastocyst stages in the 2- to 3-hour and 4- to 5-hour groups. The grading results at the eight-cell and blastocyst stages also showed that the proportion of embryos of high quality was similar among the 2- to 3-hour and 4- to 5-hour groups, and in these groups it was statistically significantly higher than the 0- to 1-hour and 8- to 9-hour groups. The results of immunofluorescence demonstrated that there was a statistically significantly higher rate of normal spindle assembly in the 2- to 3-hour and 4- to 5-hour groups.

Conclusion(s): Optimal timing of maturation benefits the development of competence of in vitro matured oocytes by promoting normal spindle assembly. (Fertil Steril (R) 2011;96:73-8. (C)2011 by American Society for Reproductive Medicine.)

语种英语
WOS记录号WOS:000292283000035
项目编号2006AA02A101 ; 2011CB944504
资助机构National High Technology Research and Development Program of China ; Ministry of Science and Technology of China
引用统计
被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56285
专题北京大学第三临床医学院
北京大学第二临床医学院_麻醉科
北京大学第三临床医学院_妇产科
北京大学第三临床医学院_生殖医学中心
作者单位1.Chinese Acad Sci, Peking Univ Third Hosp, Dept Obstet & Gynecol, Beijing 100191, Peoples R China
2.Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing 100191, Peoples R China
3.Chinese Acad Sci, Minist Educ, Key Lab Assisted Reprod, Beijing 100191, Peoples R China
4.Chinese Acad Sci, Grad Sch, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Yu, Yang,Yan, Jie,Liu, Zi C.,et al. Optimal timing of oocyte maturation and its relationship with the spindle assembly and developmental competence of in vitro matured human oocytes[J]. FERTILITY AND STERILITY,2011,96(1):73-U481.
APA Yu, Yang.,Yan, Jie.,Liu, Zi C..,Yan, Li Y..,Li, Min.,...&Qiao, Jie.(2011).Optimal timing of oocyte maturation and its relationship with the spindle assembly and developmental competence of in vitro matured human oocytes.FERTILITY AND STERILITY,96(1),73-U481.
MLA Yu, Yang,et al."Optimal timing of oocyte maturation and its relationship with the spindle assembly and developmental competence of in vitro matured human oocytes".FERTILITY AND STERILITY 96.1(2011):73-U481.
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