学科主题临床医学
Upregulation of GPR34 expression affects the progression and prognosis of human gastric adenocarcinoma by PI3K/PDK1/AKT pathway
Yu, Weidong1; Ma, Shuyun1,2; Wang, Lihong2; Zuo, Bo1,2; Li, Mei1; Qiao, Zhengguo1; Pan, Xiuying1; Liu, Yulan1,2; Wang, Jingtong2
关键词G-protein Coupled Receptor 34 (Gpr34) Gastric Cancer Metastasis Prognosis
刊名HISTOLOGY AND HISTOPATHOLOGY
2013-12-01
28期:12页:1629-1638
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology ; Pathology
研究领域[WOS]Cell Biology ; Pathology
关键词[WOS]CANCER ; METASTASIS ; LYMPHOMA ; RECEPTOR ; TARGET ; GENES ; CELLS
英文摘要

Purpose. G-protein coupled receptor 34 (GPR34), which belongs to the G-protein coupled receptors superfamily, is reportedly expressed highly in the spread of several solid tumors. However, its expression in gastric primary tumor and potential role in gastric cancer development and progression have not been determined. Methods. Immunohistochemistry, real-time RT-PCR and western blot methods were used to determine GPR34 expression in human gastric cancer tissues/cell lines and matched adjacent tissues/normal mucosal cell line. A statistical analysis was performed to establish the potential correlation between GPR34 expression and the patients′ clinicopathological characteristics, tumor progression, and prognosis. Stably transfected NCI-N87 cell lines with either GPR34 overexpression or knock-down were constructed to determine the effect of GPR34 on gastric cancer cell invasion and migration, and to explain the preliminary molecular mechanism of GPR34 in gastric cancer metastasis. Results. GPR34 is up-regulated in primary gastric cancer tissues/cell lines compared with matched adjacent tissues/normal mucosal cell line, and when the relationship between GPR34 expression and the the clinicopathological characteristics was analyzed, it was shown that GPR34 expression is significantly correlated with tumor differentiation, infiltration depth, and lymph node status and had a significant influence on prognosis. Furthermore, GPR34-overexpression increased while GPR34-knockdown inhibited NCI-N87 cell invasion in vitro by PI3K/PDK1/AKT pathway. Conclusions. Taken together, up-regulation of GPR34 expression in human gastric carcinoma may play a critical role in tumor progression and in determining patient prognosis. GPR34 may be a useful diagnostic or prognostic molecular biomarker, and a potential target for therapeutic intervention.

语种英语
WOS记录号WOS:000326879000012
项目编号30872923 ; RDB2007-47 ; RDK2008-01 ; RDB2011-26
资助机构National Natural Science Foundation of China ; University People&prime ; s Hospital Research and Development Foundations
引用统计
被引频次:11[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56307
专题北京大学第二临床医学院_临床分子生物学研究所
北京大学第一临床医学院_眼科
北京大学第二临床医学院_消化科
北京大学第二临床医学院_老年科
作者单位1.Peking Univ, Peoples Hosp, Inst Clin Mol Biol, Beijing 100871, Peoples R China
2.Peking Univ, Peoples Hosp, Dept Gastroenterol, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Yu, Weidong,Ma, Shuyun,Wang, Lihong,et al. Upregulation of GPR34 expression affects the progression and prognosis of human gastric adenocarcinoma by PI3K/PDK1/AKT pathway[J]. HISTOLOGY AND HISTOPATHOLOGY,2013,28(12):1629-1638.
APA Yu, Weidong.,Ma, Shuyun.,Wang, Lihong.,Zuo, Bo.,Li, Mei.,...&Wang, Jingtong.(2013).Upregulation of GPR34 expression affects the progression and prognosis of human gastric adenocarcinoma by PI3K/PDK1/AKT pathway.HISTOLOGY AND HISTOPATHOLOGY,28(12),1629-1638.
MLA Yu, Weidong,et al."Upregulation of GPR34 expression affects the progression and prognosis of human gastric adenocarcinoma by PI3K/PDK1/AKT pathway".HISTOLOGY AND HISTOPATHOLOGY 28.12(2013):1629-1638.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Yu, Weidong]的文章
[Ma, Shuyun]的文章
[Wang, Lihong]的文章
百度学术
百度学术中相似的文章
[Yu, Weidong]的文章
[Ma, Shuyun]的文章
[Wang, Lihong]的文章
必应学术
必应学术中相似的文章
[Yu, Weidong]的文章
[Ma, Shuyun]的文章
[Wang, Lihong]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。