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学科主题: 临床医学
题名:
Upregulation of GPR34 expression affects the progression and prognosis of human gastric adenocarcinoma by PI3K/PDK1/AKT pathway
作者: Yu, Weidong1; Ma, Shuyun1,2; Wang, Lihong2; Zuo, Bo1,2; Li, Mei1; Qiao, Zhengguo1; Pan, Xiuying1; Liu, Yulan1,2; Wang, Jingtong2
关键词: G-protein coupled receptor 34 (GPR34) ; Gastric cancer ; Metastasis ; Prognosis
刊名: HISTOLOGY AND HISTOPATHOLOGY
发表日期: 2013-12-01
卷: 28, 期:12, 页:1629-1638
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology ; Pathology
研究领域[WOS]: Cell Biology ; Pathology
关键词[WOS]: CANCER ; METASTASIS ; LYMPHOMA ; RECEPTOR ; TARGET ; GENES ; CELLS
英文摘要:

Purpose. G-protein coupled receptor 34 (GPR34), which belongs to the G-protein coupled receptors superfamily, is reportedly expressed highly in the spread of several solid tumors. However, its expression in gastric primary tumor and potential role in gastric cancer development and progression have not been determined. Methods. Immunohistochemistry, real-time RT-PCR and western blot methods were used to determine GPR34 expression in human gastric cancer tissues/cell lines and matched adjacent tissues/normal mucosal cell line. A statistical analysis was performed to establish the potential correlation between GPR34 expression and the patients′ clinicopathological characteristics, tumor progression, and prognosis. Stably transfected NCI-N87 cell lines with either GPR34 overexpression or knock-down were constructed to determine the effect of GPR34 on gastric cancer cell invasion and migration, and to explain the preliminary molecular mechanism of GPR34 in gastric cancer metastasis. Results. GPR34 is up-regulated in primary gastric cancer tissues/cell lines compared with matched adjacent tissues/normal mucosal cell line, and when the relationship between GPR34 expression and the the clinicopathological characteristics was analyzed, it was shown that GPR34 expression is significantly correlated with tumor differentiation, infiltration depth, and lymph node status and had a significant influence on prognosis. Furthermore, GPR34-overexpression increased while GPR34-knockdown inhibited NCI-N87 cell invasion in vitro by PI3K/PDK1/AKT pathway. Conclusions. Taken together, up-regulation of GPR34 expression in human gastric carcinoma may play a critical role in tumor progression and in determining patient prognosis. GPR34 may be a useful diagnostic or prognostic molecular biomarker, and a potential target for therapeutic intervention.

语种: 英语
所属项目编号: 30872923 ; RDB2007-47 ; RDK2008-01 ; RDB2011-26
项目资助者: National Natural Science Foundation of China ; University People&prime ; s Hospital Research and Development Foundations
WOS记录号: WOS:000326879000012
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56307
Appears in Collections:北京大学第二临床医学院_临床分子生物学研究所_期刊论文

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作者单位: 1.Peking Univ, Peoples Hosp, Inst Clin Mol Biol, Beijing 100871, Peoples R China
2.Peking Univ, Peoples Hosp, Dept Gastroenterol, Beijing 100871, Peoples R China

Recommended Citation:
Yu, Weidong,Ma, Shuyun,Wang, Lihong,et al. Upregulation of GPR34 expression affects the progression and prognosis of human gastric adenocarcinoma by PI3K/PDK1/AKT pathway[J]. HISTOLOGY AND HISTOPATHOLOGY,2013,28(12):1629-1638.
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