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学科主题: 临床医学
题名:
Aspirin inhibits the growth of Helicobacter pylori and enhances its susceptibility to antimicrobial agents
作者: Wang, WH; Wong, WM; Dailidiene, D; Berg, DE; Gu, Q; Lai, KC; Lam, SK; Wong, BCY
刊名: GUT
发表日期: 2003-04-01
卷: 52, 期:4, 页:490-495
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Gastroenterology & Hepatology
研究领域[WOS]: Gastroenterology & Hepatology
关键词[WOS]: NONSTEROIDAL ANTIINFLAMMATORY DRUGS ; ESCHERICHIA-COLI ; CAMPYLOBACTER-PYLORI ; ANTIBIOTIC-RESISTANCE ; OUTER-MEMBRANE ; PSEUDOMONAS-CEPACIA ; OMPF PORIN ; SALICYLATE ; PH ; EXPRESSION
英文摘要:

Background and aim: The role of Helicobacter pylori and aspirin in peptic ulcer formation and recurrence remains an important clinical topic. The interaction between aspirin and H pylori in vitro is also not clear. We investigated the effect of aspirin on the growth of H pylori and on the susceptibility of H pylori to antimicrobials.

Methods: Time killing studies of H pylori were performed with different concentrations of aspirin and salicylate. Growth of bacteria was assessed spectrophotometrically and by viable colony count. The effects of aspirin on the efficiency of colony formation and on metronidazole induced mutation to rifampicin resistance in H pylori were determined. Minimal inhibitory concentrations (MICs) of aspirin and metronidazole were tested by the standard agar dilution method. MICs of amoxycillin and clarithromycin were determined by the E test method.

Results: Aspirin and salicylate inhibited the growth of H pylori in a dose dependent manner and bactericidal activity was due to cell lysis. Aspirin 400 mug/ml caused a 2 logs decrease in colony forming units/ml at 48 hours, and suppressed the normal ability of metronidazole to induce new mutations to rifampicin. The IC90 of aspirin was 512 mug/ml. Increased susceptibility of amoxycillin, clarithromycin, and metronidazole to H pylori was observed at 1 mM (180 mug/ml) aspirin.

Conclusions: Aspirin inhibited the growth of H pylori, suppressed the mutagenic effect of metronidazole, and enhanced the susceptibility of H pylori to antimicrobial agents. This mechanism is important in future drug development for effective clearing and overcoming resistance.

语种: 英语
WOS记录号: WOS:000181828100008
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56313
Appears in Collections:北京大学第一临床医学院_消化内科_期刊论文

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作者单位: 1.Peking Univ, First Hosp, Dept Gastroenterol, Beijing, Peoples R China
2.Univ Hong Kong, Dept Med, Hong Kong, Hong Kong, Peoples R China
3.Washington Univ, Sch Med, Dept Mol Microbiol, St Louis, MO 63110 USA

Recommended Citation:
Wang, WH,Wong, WM,Dailidiene, D,et al. Aspirin inhibits the growth of Helicobacter pylori and enhances its susceptibility to antimicrobial agents[J]. GUT,2003,52(4):490-495.
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