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学科主题: 药学
题名:
Bioactivity-guided isolation of polyacetylenes with inhibitory activity against NO production in LPS-activated RAW264.7 macrophages from the rhizomes of Atractylodes macrocephala
作者: Yao, Chun-Mei; Yang, Xiu-Wei
关键词: Compositae ; Atractylodes macrocephala ; Polyacetylenes ; Anti-inflammatory ; Nitric oxide
刊名: JOURNAL OF ETHNOPHARMACOLOGY
发表日期: 2014-02-03
DOI: 10.1016/j.jep.2013.10.005
卷: 151, 期:2, 页:791-799
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Plant Sciences ; Chemistry, Medicinal ; Integrative & Complementary Medicine ; Pharmacology & Pharmacy
研究领域[WOS]: Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
关键词[WOS]: CRYSTAL-INDUCED INFLAMMATION ; ATRACTYLENOLIDE-III ; CORTICAL-NEURONS ; MELANOMA-CELLS ; KOIDZ ; PANAXYNOL ; APOPTOSIS ; GROWTH ; EXPRESSION ; CHINENSIS
英文摘要:

Ethnopharmacological relevance: The rhizome of Atractylodes macrocephala (Compositae) is one of the most well-known traditional Chinese medicine in China, Japan and Korea, which has a long history of use for the treatment of splenic asthenia, edema, anorexia, and excessive perspiration, etc. As active compounds of anti-inflammatory activity of this medicinal plant have not been fully elucidated, the aim of this study was to isolate and identify the active constituents inhibiting nitric oxide (NO) production from the rhizomes of A. macrocephala.

Materials and methods: Inhibitory activity against NO production in lipopolysaccharide-activated RAW264.7 macrophages was evaluated by Griess reaction. Fifteen polyacetylenes were isolated from the active ethyl acetate extract using activity-guided screening. The structures of all compounds were elucidated by spectroscopic methods and comparison with published data. The compounds were further tested for their inhibitory activity against NO production.

Results: Seven new polyacetylenes, named atractylodemaynes A-G (1-7), along with eight known ones (8-15) were isolated. Compound 14 was isolated for the first time from the rhizomes of A. macrocephala. The study showed that the tested compounds exhibited inhibitory activity against NO production in a dose-dependent manner. Among them, compounds 10, 11 and 12 had relatively stronger inhibitory effect with IC50 values of 28, 23 and 19 mu M, respectively.

Conclusion: The results demonstrated that the polyacetylenes might greatly contribute to the anti-inflammatory activity of the rhizomes of A. macrocephala. (C) 2013 Elsevier Ireland Ltd. All rights reserved.

语种: 英语
项目资助者: Program of Improving the Standard of Pharmacopoeia of the People&prime ; s Republic of China
WOS记录号: WOS:000331421700004
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内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56315
Appears in Collections:北京大学药学院_期刊论文

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作者单位: Peking Univ, State Key Lab Nat & Biomimet Drugs, Sch Pharmaceut Sci, Dept Nat Med,Hlth Sci Ctr, Beijing 100191, Peoples R China

Recommended Citation:
Yao, Chun-Mei,Yang, Xiu-Wei. Bioactivity-guided isolation of polyacetylenes with inhibitory activity against NO production in LPS-activated RAW264.7 macrophages from the rhizomes of Atractylodes macrocephala[J]. JOURNAL OF ETHNOPHARMACOLOGY,2014,151(2):791-799.
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