IR@PKUHSC  > 北京大学药学院
学科主题药学
Bioactivity-guided isolation of polyacetylenes with inhibitory activity against NO production in LPS-activated RAW264.7 macrophages from the rhizomes of Atractylodes macrocephala
Yao, Chun-Mei; Yang, Xiu-Wei
关键词Compositae Atractylodes Macrocephala Polyacetylenes Anti-inflammatory Nitric Oxide
刊名JOURNAL OF ETHNOPHARMACOLOGY
2014-02-03
DOI10.1016/j.jep.2013.10.005
151期:2页:791-799
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Plant Sciences ; Chemistry, Medicinal ; Integrative & Complementary Medicine ; Pharmacology & Pharmacy
资助者Program of Improving the Standard of Pharmacopoeia of the People&prime ; s Republic of China ; Program of Improving the Standard of Pharmacopoeia of the People&prime ; s Republic of China
研究领域[WOS]Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
关键词[WOS]CRYSTAL-INDUCED INFLAMMATION ; ATRACTYLENOLIDE-III ; CORTICAL-NEURONS ; MELANOMA-CELLS ; KOIDZ ; PANAXYNOL ; APOPTOSIS ; GROWTH ; EXPRESSION ; CHINENSIS
英文摘要

Ethnopharmacological relevance: The rhizome of Atractylodes macrocephala (Compositae) is one of the most well-known traditional Chinese medicine in China, Japan and Korea, which has a long history of use for the treatment of splenic asthenia, edema, anorexia, and excessive perspiration, etc. As active compounds of anti-inflammatory activity of this medicinal plant have not been fully elucidated, the aim of this study was to isolate and identify the active constituents inhibiting nitric oxide (NO) production from the rhizomes of A. macrocephala.

Materials and methods: Inhibitory activity against NO production in lipopolysaccharide-activated RAW264.7 macrophages was evaluated by Griess reaction. Fifteen polyacetylenes were isolated from the active ethyl acetate extract using activity-guided screening. The structures of all compounds were elucidated by spectroscopic methods and comparison with published data. The compounds were further tested for their inhibitory activity against NO production.

Results: Seven new polyacetylenes, named atractylodemaynes A-G (1-7), along with eight known ones (8-15) were isolated. Compound 14 was isolated for the first time from the rhizomes of A. macrocephala. The study showed that the tested compounds exhibited inhibitory activity against NO production in a dose-dependent manner. Among them, compounds 10, 11 and 12 had relatively stronger inhibitory effect with IC50 values of 28, 23 and 19 mu M, respectively.

Conclusion: The results demonstrated that the polyacetylenes might greatly contribute to the anti-inflammatory activity of the rhizomes of A. macrocephala. (C) 2013 Elsevier Ireland Ltd. All rights reserved.

语种英语
资助者Program of Improving the Standard of Pharmacopoeia of the People&prime ; s Republic of China ; Program of Improving the Standard of Pharmacopoeia of the People&prime ; s Republic of China
WOS记录号WOS:000331421700004
Citation statistics
Cited Times:12[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56315
Collection北京大学药学院
作者单位Peking Univ, State Key Lab Nat & Biomimet Drugs, Sch Pharmaceut Sci, Dept Nat Med,Hlth Sci Ctr, Beijing 100191, Peoples R China
Recommended Citation
GB/T 7714
Yao, Chun-Mei,Yang, Xiu-Wei. Bioactivity-guided isolation of polyacetylenes with inhibitory activity against NO production in LPS-activated RAW264.7 macrophages from the rhizomes of Atractylodes macrocephala[J]. JOURNAL OF ETHNOPHARMACOLOGY,2014,151(2):791-799.
APA Yao, Chun-Mei,&Yang, Xiu-Wei.(2014).Bioactivity-guided isolation of polyacetylenes with inhibitory activity against NO production in LPS-activated RAW264.7 macrophages from the rhizomes of Atractylodes macrocephala.JOURNAL OF ETHNOPHARMACOLOGY,151(2),791-799.
MLA Yao, Chun-Mei,et al."Bioactivity-guided isolation of polyacetylenes with inhibitory activity against NO production in LPS-activated RAW264.7 macrophages from the rhizomes of Atractylodes macrocephala".JOURNAL OF ETHNOPHARMACOLOGY 151.2(2014):791-799.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
谷歌学术
谷歌学术Similar articles in
[Yao, Chun-Mei]'s Articles
[Yang, Xiu-Wei]'s Articles
百度学术
百度学术Similar articles in
[Yao, Chun-Mei]'s Articles
[Yang, Xiu-Wei]'s Articles
必应学术
必应学术Similar articles in
[Yao, Chun-Mei]'s Articles
[Yang, Xiu-Wei]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.