IR@PKUHSC  > 北京大学第二临床医学院
学科主题临床医学
Robo1: A Potential Role in Ocular Angiogenesis
Huang, Lvzhen1; Xu, Yongsheng1; Yu, Wenzhen1; Li, Xiaoxin1; Chu Liqun; He, Xiangjun2; He Peiying2
关键词Angiogenesis Retina Rf/6a Robo1 Sirna
刊名CURRENT EYE RESEARCH
2009
DOI10.3109/02713680903308495
34期:12页:1019-1029
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Ophthalmology
研究领域[WOS]Ophthalmology
关键词[WOS]ENDOTHELIAL-CELLS ; AXON GUIDANCE ; EXPRESSION ; RECEPTOR ; GROWTH ; SLIT ; MIGRATION ; RETINOPATHY ; INHIBITION ; DROSOPHILA
英文摘要

Purpose: Robo1, a member of the roundabout (Robo) family, serves as neuronal guidance receptors and has been reported to mediate tumor angiogenesis. In this study, we investigated the function of Robo1 and its possible role in retinal angiogenesis in vitro and in vivo.

Methods: Expression of Robo1 in relation to retinal neovascularization was studied in an animal model of retinopathy of prematurity (ROP). siRNA technology was used to knockdown Robo1 expression to study its effects on monkey choroidal retinal endothelial cells (RF/6A) in vitro. Cell proliferation, migration, spreading, cycling, and apoptosis were assessed with the methyl thiaz olyl tetrazolium (MTT), Boyden chamber, immunohistochemistry, and flow cytometry assay. Tube formation by RF/6A on Matrigel was also analyzed.

Results: Robo1 expression was elevated in the ROP murine eyes (P<0.01). Knockdown of Robo1 expression inhibited cell proliferation and migration. Tube formation by RF/6A was also disturbed.

Conclusions: Our results show that Robo1 inhibits choroidal and retinal angiogenesis in vitro. Further studies are ongoing to evaluate Robo1 as a potential target for the treatment of choroidal or retinal angiogenesis.

语种英语
WOS记录号WOS:000272677400002
项目编号2005CB724307
资助机构National Basic Research Program of China (973 Program)
引用统计
被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56330
专题北京大学第二临床医学院
北京大学第二临床医学院_中心实验室
作者单位1.Peking Univ, Peoples Hosp, Dept Opthalmol, Beijing 100044, Peoples R China
2.Peking Univ, Peoples Hosp, Cent Lab, Beijing 100044, Peoples R China
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GB/T 7714
Huang, Lvzhen,Xu, Yongsheng,Yu, Wenzhen,et al. Robo1: A Potential Role in Ocular Angiogenesis[J]. CURRENT EYE RESEARCH,2009,34(12):1019-1029.
APA Huang, Lvzhen.,Xu, Yongsheng.,Yu, Wenzhen.,Li, Xiaoxin.,Chu Liqun.,...&He Peiying.(2009).Robo1: A Potential Role in Ocular Angiogenesis.CURRENT EYE RESEARCH,34(12),1019-1029.
MLA Huang, Lvzhen,et al."Robo1: A Potential Role in Ocular Angiogenesis".CURRENT EYE RESEARCH 34.12(2009):1019-1029.
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