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学科主题: 临床医学
题名:
Involvement of TLR2 and TLR4 and Th1/Th2 shift in inflammatory responses induced by fine ambient particulate matter in mice
作者: Zhao, Can1; Liao, Jiping1; Chu, Weili1; Wang, Suxia2; Yang, Tongsheng3; Tao, Yinghong3; Wang, Guangfa1
关键词: Inflammation ; particulate matter ; PM2.5 ; Th1/Th2 shift ; toll-like receptor
刊名: INHALATION TOXICOLOGY
发表日期: 2012-11-01
DOI: 10.3109/08958378.2012.731093
卷: 24, 期:13, 页:918-927
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Toxicology
研究领域[WOS]: Toxicology
关键词[WOS]: DIESEL EXHAUST PARTICLES ; AIR-POLLUTION PARTICLES ; OXIDATIVE STRESS ; TRANSCRIPTION FACTOR ; LINEAGE COMMITMENT ; LUNG INFLAMMATION ; IMMUNE-RESPONSE ; T-CELLS ; IN-VIVO ; DYSFUNCTION
英文摘要:

Epidemiologic studies have reported the association between fine particles (aerodynamic diameter <= 2.5 mu m; PM2.5) and health effects, but the immunological mechanisms are not clear. To investigate the dose and time-dependent role of toll-like receptor (TLR) and Th1/Th2 shift in local and systemic inflammation induced by PM2.5, mice were subjected to intratracheal instillation of 2.5, 5, or 10 mg/kg PM2.5 in this study. After 24 h, 72 h, 7 days, and 14 days, mice were sacrificed to measure TLR2 and TLR4 expressions and Th1/Th2 related cytokines in bronchoalveolar lavage fluid (BALF) and peripheral blood. Histopathological changes in lung were also examined. Inflammatory infiltration and macrophages with engulfed particles were found by lung histopathology after PM2.5 exposure. TLR4 positive cells decreased in BALF but increased in blood at 24 h after the exposure. The low percentage of TLR4 positive cells continued to day 14 in BALF, but recovered at day 7 and decreased further to lower than the control value at day 14 in blood. TLR2 positive cell changed similar to TLR4 in BALF on the dose effects. In BALF at 24 h after the exposure, the Th2 related cytokines IL-5 and IL-10 increased dose-dependently; and in blood, the Th2 related cytokines IL-4, IL-5, and IL-10 also increased. These results suggest that acute exposure of PM2.5 leads to acute inflammatory responses locally and systemically in mice. TLR2 and TLR4 are involved in this process and PM2.5 can drive a Th2-biased immune response.

语种: 英语
所属项目编号: 80170006
项目资助者: Natural Science Foundation of China
WOS记录号: WOS:000310707300004
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56336
Appears in Collections:北京大学第一临床医学院_呼吸和危重症医学科_期刊论文

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作者单位: 1.Peking Univ, Dept Resp Med, Hosp 1, Beijing 100034, Peoples R China
2.Peking Univ, Dept Electron Microscopy, Hosp 1, Beijing 100034, Peoples R China
3.Peking Univ, Dept Anim Ctr, Hosp 1, Beijing 100034, Peoples R China

Recommended Citation:
Zhao, Can,Liao, Jiping,Chu, Weili,et al. Involvement of TLR2 and TLR4 and Th1/Th2 shift in inflammatory responses induced by fine ambient particulate matter in mice[J]. INHALATION TOXICOLOGY,2012,24(13):918-927.
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