北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第一临床医学院  > 肾脏内科  > 期刊论文
学科主题: 临床医学
题名:
Antineutrophil cytoplasmic autoantibodies in patients with systemic lupus erythematosus recognize a novel 69 kDa target antigen of neutrophil granules
作者: Chen, M; Zhao, MH; Zhang, YK; Wang, HY
关键词: antineutrophil cytoplasmic autoantibodies ; autoantigens ; systemic lupus erythematosus
刊名: NEPHROLOGY
发表日期: 2005-10-01
DOI: 10.1111/j.1440-1797.2005.00441.x
卷: 10, 期:5, 页:491-495
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Urology & Nephrology
研究领域[WOS]: Urology & Nephrology
关键词[WOS]: CLINICAL ASSOCIATIONS ; DISEASE-ACTIVITY ; ANTIBODIES ; SPECIFICITY ; CLASSIFICATION ; LACTOFERRIN ; PREVALENCE ; NEPHRITIS ; ANCA
英文摘要:

Objective: Antineutrophil cytoplasmic autoantibodies (ANCA) were found in patients with systemic lupus erythematosus (SLE). Cathepsin G and lactoferrin were the major target antigens. However, some ANCA-positive sera did not recognize either of them. The present study was to investigate the unknown target antigens of ANCA in patients with SLE and their clinical significance.

Methods: Sera were collected from 72 patients with SLE. ANCA were detected in both indirect immunofluorescence and antigen-specific enzyme-linked immunosorbent assay (ELISA). Mixed neutrophil granules were separated from normal human peripheral neutrophils; soluble acid extracts in non-reducing conditions were used as antigens in western blot analysis to detect ANCA.

Results: SLE sera could blot a few bands. Interestingly, 14/72 (19.4%) sera recognized a novel 69 kDa protein band and 10/72 (13.9%) sera recognized the 55 kDa protein band, which might be bactericidal/permeability-increasing protein (BPI). The 69 kDa target antigen was different from the known target ANCA antigens such as cathepsin G and lactoferrin. Further study revealed that the percentages of patients with photosensitivity and oral ulcer in the anti-69 kDa autoantibodies-positive group were significantly higher than those in the anti-69 kDa autoantibodies-negative group (57.1%vs 10.3%, P < 0.005 and 50.0%vs 12.1%, P < 0.05, respectively).

Conclusions: A 69 kDa protein in human neutrophil granules was identified as a novel target ant !E ith SLE. The anti-69 kDa autoantibodies might be associated with photosensitivity and oral ulcer in patients with SLE.

语种: 英语
WOS记录号: WOS:000232494200014
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56337
Appears in Collections:北京大学第一临床医学院_肾脏内科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Peking Univ, Hosp 1, Div Renal, Beijing 100034, Peoples R China
2.Peking Univ, Hosp 1, Inst Nephrol, Beijing 100034, Peoples R China

Recommended Citation:
Chen, M,Zhao, MH,Zhang, YK,et al. Antineutrophil cytoplasmic autoantibodies in patients with systemic lupus erythematosus recognize a novel 69 kDa target antigen of neutrophil granules[J]. NEPHROLOGY,2005,10(5):491-495.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Chen, M]'s Articles
[Zhao, MH]'s Articles
[Zhang, YK]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Chen, M]‘s Articles
[Zhao, MH]‘s Articles
[Zhang, YK]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace