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学科主题: 临床医学
题名:
Anti-Fas antibody induces apoptosis in cultured human renal interstitial fibroblasts
作者: Cheng, LJ; Chen, YP
关键词: apoptosis ; interstitial fibroblasts ; Fas ; anti-Fas antibody
刊名: CHINESE MEDICAL JOURNAL
发表日期: 2001-02-01
卷: 114, 期:2, 页:162-166
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, General & Internal
研究领域[WOS]: General & Internal Medicine
关键词[WOS]: CELL APOPTOSIS ; IN-VIVO ; DEATH
英文摘要:

Objective To detect if Fas is expressed in human renal interstitial fibroblasts (hRIFs) and apoptosis of hRIFs can be induced by specific anti-fas antibody.

Methods hRIFs were cultured from isolated papillae of human kidney, and identified by morphologic examination, assay of antigenic components and culture in D-valine selective medium. Fas expression in normal hRIFs was detected by RT-PCR and immunocytochemistry staining. After hRIFs were incubated with interferon gamma (gamma -IFN 500 U/ml, 1000 U/ml, 1500 U/ml and 2000 U/ml, respectively) for 48 hours, Fas expression was determined by Northern blot, Western blot and flow cytometry. hRIFs pre-stimulated with gamma -IFN (500 U/ml, 48 hours) were incubated with anti-fas antibody (IgM, 0.5 mug/ml) for 12 hours. And apoptosis was identified by morphologic examination, DNA ladder assay and flow cytometry.

Results The cultured hRIFs showed a shuttle-like shape and were positively stained by labeled antivimentin antibody but negatively stained by anti-epithelial membrane antibody. They could not grow in the D-valine selective medium and died partly in a week. Fas mRNA and protein were expressed in normal hRIFs and markedly upregulated by stimulation with gamma -IFN. Apoptosis in gamma -IFN pre-stimulated hRIFs was induced by anti-fas antibody, showing cell nuclear shrinkage and condensation in morphologic feature, internucleosomal DNA fragmentation in DNA ladder assay and a pick of hypo-diploid nuclei by flow cytometry.

Conclusion Fas is normally expressed in hRIFs and can be markedly upregulated by gamma -IFN. Anti-Fas antibodies can induce apoptosis of hRIFs pre-stimulated with gamma -IFN.

语种: 英语
WOS记录号: WOS:000167246700012
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56346
Appears in Collections:北京大学第一临床医学院_肾脏内科_期刊论文

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作者单位: 1.Beijing Med Univ, Hosp 1, Div Nephrol, Beijing 100034, Peoples R China
2.China Japan Friendship Hosp, Dept Nephrol, Beijing 100029, Peoples R China

Recommended Citation:
Cheng, LJ,Chen, YP. Anti-Fas antibody induces apoptosis in cultured human renal interstitial fibroblasts[J]. CHINESE MEDICAL JOURNAL,2001,114(2):162-166.
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