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学科主题临床医学
Anti-Fas antibody induces apoptosis in cultured human renal interstitial fibroblasts
Cheng, LJ; Chen, YP
关键词Apoptosis Interstitial Fibroblasts Fas Anti-fas Antibody
刊名CHINESE MEDICAL JOURNAL
2001-02-01
114期:2页:162-166
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, General & Internal
研究领域[WOS]General & Internal Medicine
关键词[WOS]CELL APOPTOSIS ; IN-VIVO ; DEATH
英文摘要

Objective To detect if Fas is expressed in human renal interstitial fibroblasts (hRIFs) and apoptosis of hRIFs can be induced by specific anti-fas antibody.

Methods hRIFs were cultured from isolated papillae of human kidney, and identified by morphologic examination, assay of antigenic components and culture in D-valine selective medium. Fas expression in normal hRIFs was detected by RT-PCR and immunocytochemistry staining. After hRIFs were incubated with interferon gamma (gamma -IFN 500 U/ml, 1000 U/ml, 1500 U/ml and 2000 U/ml, respectively) for 48 hours, Fas expression was determined by Northern blot, Western blot and flow cytometry. hRIFs pre-stimulated with gamma -IFN (500 U/ml, 48 hours) were incubated with anti-fas antibody (IgM, 0.5 mug/ml) for 12 hours. And apoptosis was identified by morphologic examination, DNA ladder assay and flow cytometry.

Results The cultured hRIFs showed a shuttle-like shape and were positively stained by labeled antivimentin antibody but negatively stained by anti-epithelial membrane antibody. They could not grow in the D-valine selective medium and died partly in a week. Fas mRNA and protein were expressed in normal hRIFs and markedly upregulated by stimulation with gamma -IFN. Apoptosis in gamma -IFN pre-stimulated hRIFs was induced by anti-fas antibody, showing cell nuclear shrinkage and condensation in morphologic feature, internucleosomal DNA fragmentation in DNA ladder assay and a pick of hypo-diploid nuclei by flow cytometry.

Conclusion Fas is normally expressed in hRIFs and can be markedly upregulated by gamma -IFN. Anti-Fas antibodies can induce apoptosis of hRIFs pre-stimulated with gamma -IFN.

语种英语
WOS记录号WOS:000167246700012
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56346
专题北京大学第一临床医学院_肾脏内科
作者单位1.Beijing Med Univ, Hosp 1, Div Nephrol, Beijing 100034, Peoples R China
2.China Japan Friendship Hosp, Dept Nephrol, Beijing 100029, Peoples R China
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GB/T 7714
Cheng, LJ,Chen, YP. Anti-Fas antibody induces apoptosis in cultured human renal interstitial fibroblasts[J]. CHINESE MEDICAL JOURNAL,2001,114(2):162-166.
APA Cheng, LJ,&Chen, YP.(2001).Anti-Fas antibody induces apoptosis in cultured human renal interstitial fibroblasts.CHINESE MEDICAL JOURNAL,114(2),162-166.
MLA Cheng, LJ,et al."Anti-Fas antibody induces apoptosis in cultured human renal interstitial fibroblasts".CHINESE MEDICAL JOURNAL 114.2(2001):162-166.
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