|Anti-Fas antibody induces apoptosis in cultured human renal interstitial fibroblasts|
|Cheng, LJ; Chen, YP|
|关键词||Apoptosis Interstitial Fibroblasts Fas Anti-fas Antibody|
|刊名||CHINESE MEDICAL JOURNAL|
|WOS标题词||Science & Technology|
|类目[WOS]||Medicine, General & Internal|
|研究领域[WOS]||General & Internal Medicine|
|关键词[WOS]||CELL APOPTOSIS ; IN-VIVO ; DEATH|
Objective To detect if Fas is expressed in human renal interstitial fibroblasts (hRIFs) and apoptosis of hRIFs can be induced by specific anti-fas antibody.
Methods hRIFs were cultured from isolated papillae of human kidney, and identified by morphologic examination, assay of antigenic components and culture in D-valine selective medium. Fas expression in normal hRIFs was detected by RT-PCR and immunocytochemistry staining. After hRIFs were incubated with interferon gamma (gamma -IFN 500 U/ml, 1000 U/ml, 1500 U/ml and 2000 U/ml, respectively) for 48 hours, Fas expression was determined by Northern blot, Western blot and flow cytometry. hRIFs pre-stimulated with gamma -IFN (500 U/ml, 48 hours) were incubated with anti-fas antibody (IgM, 0.5 mug/ml) for 12 hours. And apoptosis was identified by morphologic examination, DNA ladder assay and flow cytometry.
Results The cultured hRIFs showed a shuttle-like shape and were positively stained by labeled antivimentin antibody but negatively stained by anti-epithelial membrane antibody. They could not grow in the D-valine selective medium and died partly in a week. Fas mRNA and protein were expressed in normal hRIFs and markedly upregulated by stimulation with gamma -IFN. Apoptosis in gamma -IFN pre-stimulated hRIFs was induced by anti-fas antibody, showing cell nuclear shrinkage and condensation in morphologic feature, internucleosomal DNA fragmentation in DNA ladder assay and a pick of hypo-diploid nuclei by flow cytometry.
Conclusion Fas is normally expressed in hRIFs and can be markedly upregulated by gamma -IFN. Anti-Fas antibodies can induce apoptosis of hRIFs pre-stimulated with gamma -IFN.
|作者单位||1.Beijing Med Univ, Hosp 1, Div Nephrol, Beijing 100034, Peoples R China|
2.China Japan Friendship Hosp, Dept Nephrol, Beijing 100029, Peoples R China
|Cheng, LJ,Chen, YP. Anti-Fas antibody induces apoptosis in cultured human renal interstitial fibroblasts[J]. CHINESE MEDICAL JOURNAL,2001,114(2):162-166.|
|APA||Cheng, LJ,&Chen, YP.(2001).Anti-Fas antibody induces apoptosis in cultured human renal interstitial fibroblasts.CHINESE MEDICAL JOURNAL,114(2),162-166.|
|MLA||Cheng, LJ,et al."Anti-Fas antibody induces apoptosis in cultured human renal interstitial fibroblasts".CHINESE MEDICAL JOURNAL 114.2(2001):162-166.|