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学科主题: 药学
题名:
Surface engineering of gold nanoparticles for in vitro siRNA delivery
作者: Zhao, Enyu1; Zhao, Zhixia1; Wang, Jiancheng1; Yang, Chunhui2; Chen, Chengjun1; Gao, Lingyan1; Feng, Qiang1; Hou, Wenjie1; Gao, Mingyuan2; Zhang, Qiang1
刊名: NANOSCALE
发表日期: 2012
DOI: 10.1039/c2nr31290e
卷: 4, 期:16, 页:5102-5109
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied
研究领域[WOS]: Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics
关键词[WOS]: RNA INTERFERENCE ; GENE DELIVERY ; QUANTUM DOTS ; VIRAL VECTORS ; CELLS ; EXPRESSION ; CANCER ; EGFR ; ELECTROLUMINESCENCE ; POLYETHYLENIMINE
英文摘要:

Cellular uptake, endosomal/lysosomal escape, and the effective dissociation from the carrier are a series of hurdles for specific genes to be delivered both in vitro and in vivo. To construct siRNA delivery systems, poly(allylamine hydrochloride) (PAH) and siRNA were alternately assembled on the surface of 11.8 +/- 0.9 nm Au nanoparticles (GNP), stabilized by denatured bovine serum albumin, by the ionic layer-by-layer (LbL) self-assembly method. By manipulating the outmost PAH layer, GNP-PAH vectors with different surface electric potentials were prepared. Then, the surface potential-dependent cytotoxicity of the resultant GNP-PAH particles was evaluated via sulforhodamine B (SRB) assay, while the surface potential-dependent cellular uptake efficiency was quantitatively analyzed by using the flow cytometry method based on carboxyfluorescein (FAM)-labeled siRNA. It was revealed that the GNP-PAH particles with surface potential of +25 mV exhibited the optimal cellular uptake efficiency and cytotoxicity for human breast cancer MCF-7 cells. Following these results, two more positively charged polyelectrolytes with different protonating abilities in comparison with PAH, i.e., polyethylenimine (PEI), and poly(diallyl dimethyl ammonium chloride) (PDDA), were chosen to fabricate similarly structured vectors. Confocal fluorescence microscopy studies indicated that siRNA delivered by GNP-PAH and GNP-PEI systems was better released than that delivered by the GNP-PDDA system. Further flow cytometric assays based on immunofluorescence staining of the epidermal growth factor receptor (EGFR) revealed that EGFR siRNA delivered by GNP-PAH and GNP-PEI exhibited similar down-regulation effects on EGFR expression in MCF-7 cells. The following dual fluorescence flow cytometry assays by co-staining phosphatidylserine and DNA suggested the EGFR siRNA delivered by GNP-PAH exhibited an improved silencing effect in comparison with that delivered by the commercial transfection reagent Lipofectamine 2000.

语种: 英语
所属项目编号: 20903100 ; 81072597 ; 81090271 ; 2007CB935801 ; 2009CB930300 ; 2011CB935800 ; 7112089
项目资助者: NSFC projects ; National Basic Research Program of China ; Beijing NSF project
WOS记录号: WOS:000306855500038
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/56357
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
2.Chinese Acad Sci, Inst Chem, Beijing 100190, Peoples R China

Recommended Citation:
Zhao, Enyu,Zhao, Zhixia,Wang, Jiancheng,et al. Surface engineering of gold nanoparticles for in vitro siRNA delivery[J]. NANOSCALE,2012,4(16):5102-5109.
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