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Surface engineering of gold nanoparticles for in vitro siRNA delivery
Zhao, Enyu1; Zhao, Zhixia1; Wang, Jiancheng1; Yang, Chunhui2; Chen, Chengjun1; Gao, Lingyan1; Feng, Qiang1; Hou, Wenjie1; Gao, Mingyuan2; Zhang, Qiang1
刊名NANOSCALE
2012
DOI10.1039/c2nr31290e
4期:16页:5102-5109
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied
研究领域[WOS]Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics
关键词[WOS]RNA INTERFERENCE ; GENE DELIVERY ; QUANTUM DOTS ; VIRAL VECTORS ; CELLS ; EXPRESSION ; CANCER ; EGFR ; ELECTROLUMINESCENCE ; POLYETHYLENIMINE
英文摘要

Cellular uptake, endosomal/lysosomal escape, and the effective dissociation from the carrier are a series of hurdles for specific genes to be delivered both in vitro and in vivo. To construct siRNA delivery systems, poly(allylamine hydrochloride) (PAH) and siRNA were alternately assembled on the surface of 11.8 +/- 0.9 nm Au nanoparticles (GNP), stabilized by denatured bovine serum albumin, by the ionic layer-by-layer (LbL) self-assembly method. By manipulating the outmost PAH layer, GNP-PAH vectors with different surface electric potentials were prepared. Then, the surface potential-dependent cytotoxicity of the resultant GNP-PAH particles was evaluated via sulforhodamine B (SRB) assay, while the surface potential-dependent cellular uptake efficiency was quantitatively analyzed by using the flow cytometry method based on carboxyfluorescein (FAM)-labeled siRNA. It was revealed that the GNP-PAH particles with surface potential of +25 mV exhibited the optimal cellular uptake efficiency and cytotoxicity for human breast cancer MCF-7 cells. Following these results, two more positively charged polyelectrolytes with different protonating abilities in comparison with PAH, i.e., polyethylenimine (PEI), and poly(diallyl dimethyl ammonium chloride) (PDDA), were chosen to fabricate similarly structured vectors. Confocal fluorescence microscopy studies indicated that siRNA delivered by GNP-PAH and GNP-PEI systems was better released than that delivered by the GNP-PDDA system. Further flow cytometric assays based on immunofluorescence staining of the epidermal growth factor receptor (EGFR) revealed that EGFR siRNA delivered by GNP-PAH and GNP-PEI exhibited similar down-regulation effects on EGFR expression in MCF-7 cells. The following dual fluorescence flow cytometry assays by co-staining phosphatidylserine and DNA suggested the EGFR siRNA delivered by GNP-PAH exhibited an improved silencing effect in comparison with that delivered by the commercial transfection reagent Lipofectamine 2000.

语种英语
WOS记录号WOS:000306855500038
项目编号20903100 ; 81072597 ; 81090271 ; 2007CB935801 ; 2009CB930300 ; 2011CB935800 ; 7112089
资助机构NSFC projects ; National Basic Research Program of China ; Beijing NSF project
引用统计
被引频次:53[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56357
专题北京大学药学院
作者单位1.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
2.Chinese Acad Sci, Inst Chem, Beijing 100190, Peoples R China
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GB/T 7714
Zhao, Enyu,Zhao, Zhixia,Wang, Jiancheng,et al. Surface engineering of gold nanoparticles for in vitro siRNA delivery[J]. NANOSCALE,2012,4(16):5102-5109.
APA Zhao, Enyu.,Zhao, Zhixia.,Wang, Jiancheng.,Yang, Chunhui.,Chen, Chengjun.,...&Zhang, Qiang.(2012).Surface engineering of gold nanoparticles for in vitro siRNA delivery.NANOSCALE,4(16),5102-5109.
MLA Zhao, Enyu,et al."Surface engineering of gold nanoparticles for in vitro siRNA delivery".NANOSCALE 4.16(2012):5102-5109.
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