IR@PKUHSC  > 北京大学第三临床医学院  > 眼科
学科主题临床医学
Glial cell-derived neurotrophic factor gene delivery enhances survival of human corneal epithelium in culture and the overexpression of GDNF in bioengineered constructs
Qi, Hong1,4; Shine, H. David2,3; Li, De-Quan1; de Paiva, Cintia S.1; Farley, William J.1; Jones, Dan B.1; Pflugfelder, Stephen C.1
关键词Cornea Epithelium Glial Cell-derived Neurotrophic Factor Gene Therapy Adenoviral Vector Bioengineering
刊名EXPERIMENTAL EYE RESEARCH
2008-12-01
DOI10.1016/j.exer.2008.09.012
87期:6页:580-586
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Ophthalmology
研究领域[WOS]Ophthalmology
关键词[WOS]EXPANDED EX-VIVO ; TARGETED TRANSDUCTION ; TRANSGENE EXPRESSION ; HUMAN LIMBAL ; STEM-CELLS ; IN-VIVO ; SURFACE ; LINE ; TRANSPLANTATION ; THERAPY
英文摘要

This paper evaluates the effects of adenoviral vector-mediated glial cell-derived neurotrophic factor (GDNF) gene delivery on survival of primary human corneal epithelial cells (PHCEC) established from limbal explants in vitro and the overexpression of GDNF gene in bioengineered human corneal constructs on substrate of corneal stromal discs followed by autograft ex vivo. In vitro, the overexpression of GDNF in the supernatant of PHCEC peaked at day 4, but lasted for at least 4 weeks after the transduction mediated by adenoviral vector. At day 10, the cell viability was 2-fold greater (P < 0.001), the number of terminal deoxynucleotidyl transferase-mediated dUTP-digoxigenin nick end labeling (TUNEL)-positive cells was more than 50% lower (P < 0.01) in the GDNF transduction group than the non-transduction group. 5 weeks after the transduction, the living cell population was greater in the GDNF transduction group than the non-transduction group (P < 0.01). In the ex vivo autograft of the bioengineered human corneal constructs, outgrowth of enhanced green fluorescent protein (eGFP) positive cells on the recipient corneoscleral tissue was observed. Overexpression of GDNF in the supernatant peaked at day 2, but was observed for at least 4 weeks after transplantation. At day 5, immunofluorescent staining showed expression of GDNF by all layers of epithelial cells on the graft. Our findings revealed that GDNF is a survival growth factor for cultured human corneal epithelium. The use of bioengineered human corneal constructs containing GDNF-transduced epithelial cells represents a novel method for delivering of this gene to promote survival of transplanted corneal epithelium to treat various corneal surface diseases. (C) 2008 Elsevier Ltd. All rights reserved.

语种英语
WOS记录号WOS:000261823900012
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/56378
专题北京大学第三临床医学院_眼科
作者单位1.Baylor Coll Med, Dept Ophthalmol, Ocular Surface Ctr, Cullen Eye Inst, Houston, TX 77030 USA
2.Baylor Coll Med, Ctr Cell & Gene Therapy, Houston, TX 77030 USA
3.Baylor Coll Med, Dept Neurosurg, Houston, TX 77030 USA
4.Peking Univ, Peking Univ Hosp 3, Peking Univ Eye Ctr, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Qi, Hong,Shine, H. David,Li, De-Quan,et al. Glial cell-derived neurotrophic factor gene delivery enhances survival of human corneal epithelium in culture and the overexpression of GDNF in bioengineered constructs[J]. EXPERIMENTAL EYE RESEARCH,2008,87(6):580-586.
APA Qi, Hong.,Shine, H. David.,Li, De-Quan.,de Paiva, Cintia S..,Farley, William J..,...&Pflugfelder, Stephen C..(2008).Glial cell-derived neurotrophic factor gene delivery enhances survival of human corneal epithelium in culture and the overexpression of GDNF in bioengineered constructs.EXPERIMENTAL EYE RESEARCH,87(6),580-586.
MLA Qi, Hong,et al."Glial cell-derived neurotrophic factor gene delivery enhances survival of human corneal epithelium in culture and the overexpression of GDNF in bioengineered constructs".EXPERIMENTAL EYE RESEARCH 87.6(2008):580-586.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Qi, Hong]的文章
[Shine, H. David]的文章
[Li, De-Quan]的文章
百度学术
百度学术中相似的文章
[Qi, Hong]的文章
[Shine, H. David]的文章
[Li, De-Quan]的文章
必应学术
必应学术中相似的文章
[Qi, Hong]的文章
[Shine, H. David]的文章
[Li, De-Quan]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。